مطالعه اثر پدیوکوکوس پنتوساسئوس (Pediococcus pentosaceus) بر ساختار هیستومورفومتری روده بچه ماهی قزل آلای رنگین کمان (Oncorhynchus mykiss Walbaum, 1792)

نوع مقاله: مقاله کامل

نویسندگان

1 گروه علوم پایه دانشکده دامپزشکی دانشگاه تهران، تهران، ایران

2 گروه شیلات، واحد تالش، دانشگاه آزاد اسلامی، تالش، ایران

3 موسسه تحقیقات بین المللی تاسماهیان دریای خزر، سازمان تحقیقات، آموزش و ترویج کشاورزی ، رشت ، ایران.

4 دانش آموخته دوره دکترای حرفه ای دامپزشکی دانشگاه تهران، تهران، ایران.

چکیده

در دهه اخیر مطالعات زیادی در رابطه با ارزیابی اثرات پروبیوتیک‌ها در ماهیان صورت گرفته است و اثرات مثبت آن بر روی پارامترهای رشد و سلامت ماهیان گزارش شده است. قزل‌آلای رنگین کمان از گونه‌های پرورشی محسوب می‌شود که از لحاظ اقتصادی حائز اهمیت می‌باشد. در این مطالعه بچه‌ماهی قزل‌آلای رنگین‌کمان در یک گروه کنترل (بدون باکتری در غذا) و سه گروه تیمار با پودر باکتری پدیوکوکوس پنتوساسئوس با شمارش 108 باکتری (CFU) به میزان 200 ، 300 و 400 گرم باکتری به ازای هر تن غذا استفاده شد. پس از گذشت 8 هفته از دوره آزمایش، نمونه‌های بافتی روده از قسمت‌های مختلف برداشت و در فرمالین 10 درصد ثابت‌سازی شد. پس از آماده‌سازی بافتی، رنگ‌آمیزی مقاطع به کمک هماتوکسیلین-ائوزین و تری کروم ماسون صورت گرفت. نتایج نشان داد که پارامترهای هیستومتری نظیر ارتفاع و عرض پرز در روده قدامی و میانی گروه‌های تیمار خصوصا گروه 400 گرم باکتری به ازای هر تن غذا نسبت به گروه کنترل افزایش معنی‌داری (05/0>p) داشته است. لذا بنظر می‌رسد پروبیوتیک پدیوکوکوس پنتوساسئوس می‌تواند تاثیرات مثبت روی رشد و تکثیر بافت روده داشته و منجربه افزایش سطح جذب و رشد بچه ماهیان قزل آلا گردد.

کلیدواژه‌ها


عنوان مقاله [English]

Study of Pediococcus pentosaceus effect on histomorphometrical structure of intestine in juvenile rainbow trout (Oncorhynchus mykiss Walbaum , 1792)

نویسندگان [English]

  • J. Sadeghinezhad 1
  • S. J. Abolghasemi 2
  • A.R. Shenavar Masouleh 3
  • N. Zadsar 4
1 Department of basic sciences, Faculty of veterinary medicine, University of Tehran, Tehran, Iran.
2 Department of Fisheries, Talesh Branch, Islamic Azad University, Talesh, Iran.
3 Department of Aquatic Animal Health, International Sturgeon Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran.
4 Faculty of veterinary medicine, University of Tehran, Tehran, Iran.
چکیده [English]

In recent decades, many studies have been conducted evaluating the effects of probiotics in fish. Its positive effects on growth performance and the health of fish have been reported. Rainbow trout is one of the aquaculture species which is important in economic aspect. Juvenile rainbow trout were used in one control group (without bacteria in food) and three treatment groups with108 CFU/g Pediococcus pentosaceus in different doses of 200,300,400 g/ 1000 kg food were used in this study. After eight weeks, tissue samples from different parts of the intestine have been taken and then fixed in 10 % formalin. Then, tissue passages were stained with hematoxylin-eosin, and Masson’s trichrome. The results showed that histometrical values like height and width of the villi in anterior and middle intestine of treatment groups especially in 400 g bacteria / 1000 kg food was significantly (p<0.05) increased in comparison with that of control group. It seems that Pediococcus pentosaceus caused positive effects on proliferation and growth of the intestine tissue which lead to increase of absorption surface area and growth of the juvenile rainbow trout.

کلیدواژه‌ها [English]

  • Pediococcus pentosaceus
  • Probiotic
  • Morphology
  • intestine
  • Rainbow trout

1- Abrams, G.D., H. Bauer and Sprinz, H. 1963. Influence of the normal flora on mucosal morphology and cellular renewal in the ileum. A comparison of germ-free andconvenventional mice. Laboratory investigation 12: 355-364.
2- Aubin, J., F. Gatesoupe, L. Labbe and L. Lebrun. 2005. Trial of probiotics to prevent the vertebral column compression syndrome in rainbow trout (Oncorhynchus mykiss Walbaum). Aquaculture research 36: 758-767.
3- Balcazar, J.L., I. de Blas, I. Ruız-Zarzuela, D. Cunningham, D. Vendrell and J. L. Muzquiz. 2006. The role of probiotics in aquaculture. Veterinary microbiology 114: 173–186.
4- Banasaz, M., E. Norin and T. Midtvedt. 2001. The role of gender, age and microbial status on cell kinetics in the gastrointestinal tract of mice. Microbial ecology in health and disease 13: 135–142.
5- Banasaz, M., E. Norin, R. Holma, T. Midtvedt. 2002. Increased enterocyte production in gnotobiotic rats mono-associated with Lactobacillus rhamnosus GG. Applied and environmental microbiology 68: 3031–3034.
6- Bagheri, T., S. A. Hedayati, V. Yayari, M. Alizade and A. Farzanfar. 2008. Growth, survival and gut microbial load of rainbow trout (Onchorhynchus mykiss) fry given diet supplemented with probiotic during the two months of first feeding. Turkish journal of fisheries and aquatic science 8: 43-48.
7- Buts, J. P., N. De Keyser, S. Marandi, D. Hermans, E. M. Sokal, Y. H. Chae, Y. H., L. Lambotte, H. Chanteux and P. M. Tulkens. 1999. Saccharomyces boulardii upgrades cellular adaptation after proximal enterectomy in rats. Gut 45: 89-96.
8- Carnevali, O., M. C. Zamponi, R. Sulpizio, A. Rollo, M. Nardi, C. Orpianesi, S. Silvi and M. Caggiano. 2004. Administration of probiotic strain to improve sea bream wellness during development. Aquaculture international 12: 377– 386.
9- De Schrijver, R. and F. Ollevier. 2000. Protein digestion in juvenile turbot (Scophthalmus maximus) and effects of dietary administration of Vibrio proteolyticus. Aquaculture 186: 107-116.
10- Díaz-Rosales, P., S. Arijo, M. Chabrillon, F. J. Alarzon, S. T. Tapia-Paniagua and E. Martínez-Manzanares . 2009. Effects of two closely related probiotics on respiratory burst activity of Senegalese sole (Solea senegalensis, Kaup) phagocytes, and protection against Photobacterium damselae subsp. Piscicida. Aquaculture international 293: 16-21.
11- Diaz, J.P., E. Guyot, S. Vigier and R. Connes. 1997. First events in lipid absorption during post-embryonic development of the anterior intestine in gilt-head sea bream. Journal of fish biology 51: 180–192.
12- El- Haroun, E. R., A. M. A. S. Goda and M. A. Kabir Chowdhury. 2006. Effect of dietary probiotic Biogen- supplementation as a growth promoter on growth performance and feed utilization of Nile tilapia Oreochromis niloticus. Aquaculture research 37: 1473-1480.
13- Ferguson, R. M. W., D. L. Merrifield, G. M. Harper, M. D. Rawling, S. Mustafa and S. Picchietti. 2010. The effect of Pediococcus acidilactici on the gut microbiota and immune status of on-growing red tilapia (Oreochromis niloticus). Journal of applied microbiology 109: 851-862.
14- Grisez, L. and F. Ollevier. 1995. Vibrio (Listonella) anguillarum infections in marine fish larviculture. P. 497, In: Lavens P., E. Jaspers, I. Roelands (ed.), European Aquaculture Society, Gent (Special publication N. 24).
15- Gunasekara, R. A., P. Cornillie, C. Casteleyn, W. De Spiegelaere, P. Sorgeloos, P. Simoens, P. Bossier and W. Van den Broeck. 2011. Stereology and computer assisted three-dimensional reconstruction as tools to study probiotic effects of Aeromonas hydrophila on the digestive tract of germ-free Artemia franciscana nauplii. Journal of applied microbiology 110: 98-105.
16- Kristiansen, M., D. L. Merrifield, J. L. Gonzalez Vecino, R. Myklebust and E. Ringø, E. 2011. Evaluation of Prebiotic and Probiotic Effects on the Intestinal Gut Microbiota and Histology of Atlantic salmon (Salmo salar L.). Journal of aquaculture research and development, S1: 1-8.
17- Manning, M. J., M. F. Grace and C. J. Secombes. 1982. Developmental aspects of immunity and tolerance in fish. Pp. 31-46, In: Roberts, R. J. (ed.), Microbial disease of fish. Academic Press, London.
18- Merrifield, D. L., G. Bradley, G. M. Harper, R. T. M. Baker, C. B. Munn and S. J. Davies, S. J. 2011. Assessment of the effects of vegetative and lyophilized Pediococcus acidilactici on growth, feed utilization, intestinal colonization and health parameters of rainbow trout (Oncorhynchus mykiss Walbaum). Aquaculture Nutrition 17: 73-79.
19- Merrifield, D. L., G. Harper, A. Dimitroglou, E. Ringø and S. J. Davies. 2010a. Possible influence of probiotic adhesion to intestinal mucosa on the activity and morphology of rainbow trout (Oncorhynchus mykiss) enterocytes. Aquaculture research 41: 1268-1272.
20- Merrifield, D. L., A. Dimitroglou, A. Foey, S. J. Davies, R. T. M. Baker and J. Bøgwald. 2010b. The current status and future focus of probiotic and prebiotic applications for salmonids. Aquaculture 302: 1-18.
21- Moslehi, F., M. Sattari and A. Shenavar Masouleh. 2016. Effects of Pediococcus pentosaceus as a probiotic on intestinal microbiota and body composition of Siberian sturgeon, Acipenser baerii Brandt, 1869. International Journal of Aquatic Biology 4: 10-15.
22- Palay, S.L. and L. J. Karlin. 1959. An electron microscopic study of the intestinal villus ii. The pathway of fat absorption. The journal of biophysical and biochemical cytology 5: 373–384.
23- Panigrahi, A., V. Kiron, T. Kobayashi, J. Puangkaew, S. Satoh, A. Rekecki, K. Dierckens, S. Laureau, N. Boon, P. Bosier and W. Van den Broeck. 2009. Effect of germ-free environment on survival and gut development of larval sea bass (Dicentrarchus labrax L.). Aquaculture 293: 8–15.
24- Ramos, M. A., J. F. Gonçalves, S. Batista, B. Costas, M. A. Pires, P. Rema and R. O. Ozório. 2015. Growth, immune responses and intestinal morphology of rainbow trout (Oncorhynchus mykiss) supplemented with commercial probiotics. Fish and shellfish immunology: 45: 19-26.
25- Rawls, J.F., B. S. Samuel, B.S. and J. I. Gordon. 2004. Gnotobiotic zebrafish reveal evolutionarily conserved responses to the gut microbiota. Proceeding of national academy of Science 101: 4596–4601.
26- Ringo, E., R. Myklebust, T. M. Mayhew and R. E. Olsen. 2007a. Bacterial translocation and pathogenesis in the digestive tract of larvae and fry. Aquaculture: 268: 251-264.
27- Ringø, E., I. Salinas, R. E. Olsen, A. Nyhaug, R. Myklebust and T. M. Mayhew. 2007b. Histological changes in intestine of Atlantic salmon (Salmo salar L.) following in vitro exposure to pathogenicand probiotic bacterial strains. Cell and tissue research 328: 109-116.
28- Ringø, E., S. Sperstad, R. Myklebust, S. Refstie and A. Krogdahl. 2006. Characterisation of the microbiota associated with the intestine of Atlantic cod (Gadus morhua L.). The effect of fish meal, soybean meal and a bioprocessed soybean meal. Aquaculture 261: 829-841.
29- Saenz de Rodriganez, M. A., Diaz-Rosales, P., Chabrillon, M., Smidt, H., Arijo, S., Leon-Rubio, J. M., F. J. Alarcon, M. C. Balebona, M. A. Morinigo, J. B. Cara and F. J. Moyano. 2009. effect of dietary administration of probiotics on growth and intestine functionality of juvenile Senegalese sole (Solea senegalensis, Kaup 1858). Aquaculture nutrition 15: 177-185.
30- Salinas, I., A. Cuesta, M. A. Esteban and J. Meseguer. 2005. Dietary administration of Lactobacillus delbru¨eckii and Bacillus subtilis, single or combined, on gilthead seabream cellular innate immune responses. Fish and shellfish immunology 19: 67-77.
31- Salma, W., Z. Zhou, W. Wang, F. Askarian, A. Kousha, M. Tajabadi Ebrahimi, R. Myklebust and E. Ringo. 2011. Histological and bacteriological changes in intestine of beluga (Huso huso) following ex vivo exposure to bacterial strains. Aquaculture 314: 24-33.
32- Soltani, M., A. Shenavar Masouleh, M. Ahmadi, M. Pourkazemi, and A. Taherimirghaed. 2015. Antibacterial activity, antibiotic susceptibility and probiotic use of lactic acid bacteria (LAB) in Persian sturgeon (Acipenser persicus). Iranian Journal of Aquatic Animal Health 2: 54-65.
33- Soltani, M., M. Pourkazemi, M. R. Ahmadi1, A. Taherimirghead, D. L. Merrifield and A. S. Masouleh. 2013. Genetic diversity of lactic acid bacteria in the intestine of Persian sturgeon fingerlings. Journal of Applied Ichthyology 29: 494–498.
34- Thyssen, A. and F. Ollevier. 2001. In vitro antimicrobial susceptibility of Photobacterium damselae subsp. piscicida to 15 different antimicrobial agents. Aquaculture 200: 259–269.
35- Timmermans, L. P. M. 1987. Early development and differentiation in fish. Sarsia 72: 331-339.
36- Vazquez, J. A., M. P. Gonzalez and M. A. Murado. 2005. Effects of lactic acid bacteria cultures on pathogenic microbiota from fish. Aquaculture 245: 149-161.