اثر استفاده از بنتونیت و نشاسته گندم بر عملکرد، خصوصیات لاشه، پارامترهای خونی و جمعیت میکروبی در جوجه‌های گوشتی

نوع مقاله : مقاله کامل

نویسندگان

1 دانشگاه علوم کشاورزی و منابع طبیعی ساری

2 عضو هیات علمی دانشگاه علوم کشاورزی و منابع طبیعی ساری

چکیده

این آزمایش به‌منظور بررسی تاثیر مکمل بنتونیت سدیم و نشاسته گندم بر عملکرد، خصوصیات لاشه، پارامترهای خونی، pH قسمت‌های مختلف دستگاه گوارش و بستر، جمعیت میکروبی ایلئوم و مرفولوژی روده در جوجه‌های گوشتی در قالب طرح کاملا تصادفی با 4 تیمار و 5 تکرار انجام شد. در این آزمایش 200قطعه جوجه گوشتی یکروزه سویه راس 308 بصورت 10 قطعه بصورت مخلوط از دوجنس در هر تکرار(پن) قرار داده شدند تیمارها شامل: 1- جیره شاهد 2- جیره حاوی بنتونیت 3- جیره حاوی نشاسته4 -جیره حاوی نشاسته و بنتونیت بود که تا پایان 42 روزگی آب و خوراک به صورت آزاد در اختیار پرندگان قرار داشت. میزان خوراک مصرفی و اضافه وزن جوجه‌های هر تکرار بصورت هفتگی محاسبه و جهت اندازه‌گیری پارامترهای خونی از سیاهرگ بال پرنده خون‌گیری شد. تیمارهای آزمایش در طی دوره‌های زمانی مختلف دوره پرورش بر روی وزن بدن و ضریب تبدیل خوراک تاثیر معنی‌داری نداشتند. در طی دوره 1- 10 روزگی (دوره استارتر) خوراک مصرفی به طور معنی‌داری تحت تاثیر تیمارهای آزمایشی قرار گرفت (0.05>P). اثر اضافه کردن بنتونیت و نشاسته روی خصوصیات لاشه معنی‌دار نبود(0.05>P). نتایج این آزمایش نشان داد که استفاده از بنتونیت و نشاسته روی کلسترول، تری گلیسیرید و VLDL تاثیر معنی‌داری نداشت اما روی گلوکز تاثیر داشت. اثر تیمارهای آزمایشی بر روی PDI (شاخص اندازه‌گیری مقاومت پلت ) در دوره‌ای آزمایش آغازین و رشد معنی‌دار بوده است. تیمارهای آزمایشی مختلف بر روی جمعیت میکروبی تاثیر معنی‌داری نداشته است(0.05<P). نتایج نشا داد که بین تیمارها بر روی ارتفاع پرز، و نسبت ارتفاع پرز به عمق کریپت در ایلئوم روده باریک معنی‌دار نبوده است. ولی ضخامت پرز و سطح پرز تحت تاثیر معنی‌دار قرار گرفتند(0.05<P).

کلیدواژه‌ها


عنوان مقاله [English]

Effects of bentonite and wheat starch on performance, carcass characteristics, blood parameters ilium microbial population in broilers

نویسندگان [English]

  • M. Ebrahimi 1
  • M. Kazemi-Fard 2
  • M. Rezaei 2
  • E. Dirandeh 2
1 Sari agricultural and natural resources university
2 Academic members of animal science, Sari agricultural and natural resources university
چکیده [English]

To consider the effect of supplementation bentonite and wheat starch on performance, carcass characteristics, blood parameters and value pH of gastrointestinal tract and litter and ilium microbial population intestinal morphology characteristics of broilers, a completely randomized design test was done with four treatments and five replicate for each treatment. In this experimental Two hundred one day-old mixed sex broilers (Ross breed) were divided into four treatments with 10 chicks per each replicate (pen). Treatments were contain: 1- Basal diet without additive (control) 2- 2- Basal diet plus bentonite 3- Basal diet plus wheat starch 4- Basal diet plus bentonite and wheat starch. The diets were fed to broilers at 42 days and water and feed were fed ad-libitum. Range of feed intake and gain of each replicate were weighted weekly and took blood to determine blood parameters from wing vein of birds. The results showed no significant difference between the experimental groups on gain body and feed conversion ratio but in starter period significantly affect feed intake. Addition of bentonite and wheat starch to diet had not significant effect carcass characteristics. The result of this experiment showed during the whole period of experiment, using bentonite and wheat starch no had significantly effect on cholesterol, triglyceride and VLDL of serum but effect on glucose serum. Treatments significantly effect on PDI in starter and grower period. Effect Experimental treatments no had significant on microbial population. Result indicated that between treatments no had significant on villi length and villus/crypt ratio but were statistically significant (p < 0.05) villi thickness and level.

کلیدواژه‌ها [English]

  • bentonite
  • wheat starch
  • performance
  • broiler
1- Bailey, C. A., Latimer, G. W. Barr, A. C. Wigle, W. L. Haq, A. U. Balthrop, J. E. and Kubena. L.F. (2006) Efficacy of montmorillonite clay (Novasil PLUS) for protecting Full-Term broilers from aflatoxicosis. J. Appl. Poult. Res. 15, 198-206.
2- Ball, M.E.E., Owens, B. McCracken, K.J. (2013) Chemical and physical predictors of the nutritive value of wheat in broiler diets. Asian-Aust. J. Anim. Sci. 26, 97–107.
3- Björck, I., Liljeberg, H. and Östman. E. (2000) Low glycemic-index foods. Br. J. Nutr. 83(Suppl. 1):S149–S155.
4- Carré B., Maisonnier, S. Melcion, J.P. Oury, F.X. Gomez, J. and Pluchard, P. (2002) Relationships between digestibility of food components and characteristics of wheats (Triticum aestivum) introduced as the only cereal source in a broiler chicken diet. Br. Poult. Sci. 43, 404–415.
5- Diaz, D. E., and Smith. T. K. (2005) Mycotoxin sequestering agents: practical tools for the neutralization of mycotoxins. Pages 323-339 in the Mycotoxin Blue Book.Duarte Diaz, ed. Nottingham University Press, Nottingham, UK.
6- Droy-lefain, M. T., Drout, Y. and Schatz. B. (1985) Sodium glycodeoxycholate and spin ability of gastrointestinal mucus: productive effect of smectite. Gastroenterology 88(supple.2)1369 (abstract) .
7- Eliasson, A.C., Gudmundsson, M. (1996) Starch: physicochemical and functional aspects. In: Eliasson, A.C. (Ed.), Carbohydrates in Food. Marcel Dekker, New York, pp. 431–503.
8- Englyst, H. N., Veenstra, J. and Hudson. G. (1996) Measurement of rapidly available glucose (RAG) in plant foods: A potential in vitro predictor of the glycaemic response. Br. J. Nutr. 75:327– 337.
9- Englyst, H. N., Wiggins, H. S. Cummings, J. H. (1982) Determination of the non-starch polysaccharides in plant foods by gas-liquid chromatography of constituent sugars as alditol acetates. Analyst 107, 307–318.
10- Harvey, R.B., Kubena, L.F. Elissalde, M.H. and Phillips. T.D. (1993) Efficacy of zeolite ore compounds on the toxicity of aflatoxin to growing broiler chickens. Avian Dis 37: 67-73.
11- Hetland, H., Svihus, B. and Olaisen. V. (2002) Effect of feeding whole cereals on performance, starch digestibility and duodenal particle size distribution in broiler chickens. Br. Poult. Sci. 43:416–423.
12- Huwing, A., Fremund, S. Kappeli, O. and Dutler. H. (2001) Mycotoxin detoxication of animal feed by different adsorbents. Toxicol. Lett. 122: 179-188.
13- Incharoen, T., Khambualai, O. and Yamauchi. k. (2009) Performance and histological changes of intestinal villi in chickens fed dietary natural zeolite in clouding plant extract. Asian J. Poult. Sci. 3: 42-50.
14- Izat, A. L., Adams, M. H. Cabel, M. C. Colberg, M. Reiber, M. A. Skinner, J. T. and Waldroup. P. W. (1990) Effects of formic acid or calcium formate in feed on performance and microbiological characteristics of broilers. Poult. Sci. 69(11), 1876-1882 .
15- Jaroni, D., Scheideler, S. E. Beck, M. and Wyatt. C. (1999). The effect of dietary wheat middling's and enzyme supplementation. 1. Late egg production efficiency, egg yields, and egg composition in two strains of Leghorn hens. Poult. Sci. 78(6), 841-847.
16- Kermanshahi, H., Hazegh, A.R. and Afzali. N. (2009) Effect of sodium bentonite in broiler chickens fed diets contaminated with aflatoxin B1. J. Anim. Vet. Adv. 8: 1631-1636.
17- Kleessen, B., Stoof, G. Proll, J. Schmiedl, D. Noack, J. and Blaut. M. (1997) Feeding resistant starch affects fecal and cecal microflora and short-chain fatty acids in rats. J. Anim. Sci. 75:2453–2462.
18- Leeson, S., and Summers. J. D. (1997). Feeding programs for broilers. Commercial poultry nutrition. 3: 207-298.‏
19- Machacek, M., Veserek, V. Mas, N. Suchy, P. Strakova, E. Serman, V. and Herzig, I. (2010) Effect of the Feed Additive Clinoptilolite (ZeoFeed) on Nutrient Metabolism and Production Performance of Laying Hens. Acta. Vet. BRNO. 79: 29-34.
20- Maisonnier, S., Gomez, J. and Carré. B. (2001) Nutrient digestibility and intestinal viscosities in broiler chickens fed on wheat diets, as compared to maize diets with added guar gum. Br. Poult. Sci., 42, 102–110.
21- Marquez, R. N. M., and Hernandez. I. T. (1995) Aflatoxin adsorbent capacity of two Mexican aluminosilicates in experimentally contaminated chick diet. Food Addit. Contam. A 12:431–433.
22- Miazzo, R., Peralta, M. F. Magnoli, C. Salvano, M. Ferrero, S.S. Chiacchiera, M. Carvalho, C. A. and Dicer. A. (2005) Efficacy of sodium bentonite as a detoxifier of broiler feed contaminated with aflatoxin and fumonisin. Poult. Sci. 84:1–8.
23- Miazzo, R., Rosa, C. A. R Queiroz, E. C. D. Carvalho, C. Magnoli, D. and Chiacchiera. S. M. (2000) Efficacy ofsynthetic zeolite to reduce the toxicity of aflatoxin in broiler chicks. Poult. Sci. 79: 1-6.
24- Montagne, L., Pluske, J. R. and Hampson. D. J. (2003) A review of interactions between dietary fiber and the intestinal mucosa, and their consequences on digestive health in young non-ruminant animals. Anim. Feed Sci. Technol. 108, 95-11.
25- Moran E.T. (1985) Digestion and absorption of carbohydrates in fowl and events through perinatal development. J. Nutr., 115, 665–674.
26- Mumpton, F.A., and Fishman. P.H. (1977) the application of natural zeolites in animal science and aquaculture. J. Anim. Sci. 45: 1188-1203.
27- Oguz, H., Kececi, T. Birdane, Y.O. Onder, F. and Kurtoglu. V. (2000) Effect of clinoptilolite on serum biochemical and haematological characters of broiler chickens during aflatoxicosis. Res Vet Sci. 69:89-93.
28- Pasha, T.N., Farooq, M.U. Khattak, F.M. Jabbar, M.A. and Khan, A.D. (2007) Effectiveness of sodium bentonite and two commercial products as aflatoxin absorbents in diets for broiler chickens. Anim. Feed Sci. Technol. 132: 103-110.
29- Petkova, G., and Ivonov. K. (1982) Effect of adding bentonite to mixed forages for broiler chickens. Zhivotnov dni Nauki. 19: 69-76.
30- Plavnik, I., and Sklan. D. (1995) Nutritional effects of expansion and short time extrusion on feeds for broilers. Anim. Feed Sci. Technol. 55:247–251.
31- Quisenberry, J. H., & Bradley, J. W. (1964). Sodium bentonite feeding experiments. Feedstuffs, 36(47), 23.
32- Riesenfeld G., Sklan, D. Bar, A. Eisner, U. and Hurwitz. S. (1980) Glucose absorption and starch digestion in the intestine of the chicken. J. Nutr. 110, 117–121.
33- Sahoo, S. P. (2016) Effect of chemically amended litter on litter quality and broiler performance in winter. Journal of Applied Animal Research. 44: 1-5.
34- Salari, S., Kermanshahi, H. and Nasiri Moghaddam. M. (2006) H. Effect of sodium bentonite and comparison of pellet vs. mash on performance of broiler chickens. Int. J. Poult. Sci. 5: 31- 34.
35- Southern, L.L., Ward, T. L. Bidner, T. D. and Hebert. L.G. (1994) Effects of sodium bentonite or hydrated sodium calcium aluminosilicate on growth performance and tibia mineral concentrations in broiler chicks fed nutrient deficient diets. Poult. Sci. 73: 848-854.
36- Stevnebo, A., Sahlstrom, S. and Svihus. B. (2006) Starch structure and degree of starch hydrolysis of small and large starch granules from barley varieties with varying amylase content. Anim. Feed Sci. Technol. 130, 23-38.
37- Svihus B., Herstad, O. Newman, C.W. and Newman. R.K. (1997) Comparison of performance and intestinal characteristics of broiler chickens fed on diets containing whole, rolled or ground barley. Br. Poult. Sci. 38: 524-529.
38- Svihus, B. (2001) Research note: a consistent low starch digestibility observed in pelleted broiler chicken diets containing high levels of different wheat varieties. Anim. Feed. Sci. Tech., 92, 45–50.
39- Tatar, A., Boldaji, F. Dastar, B. and Yaghobfar. A. (2008) Comparison of different levels of zeolite on serum characteristics, gut pH, apparent digestibility of crude protein and performance of broiler chickens. P. 235. International Zeolite Conference. Tehran, Iran.
40- Tauqir, N. A., Sultan, G. I. and Nawaz. H. (2001). Effect of different levels of bentonite with varying energy levels on the performance of broilers.‏
41- Thomas, D. V., Ravindran, V. and Ravindran. G. (2008) Nutrient digestibility and energy utilisation of diets based on wheat, sorghum or maize by the newly hatched broiler chick. Br. Poult. Sci. 49:429–435.
42- Trckova, M., Matlova, L., and Dvorska, L. (2004). Kaolin, bentonite, and zeolites as feed supplements for animals: health advantages and risks. A review. Veterinarni Medicina-UZPI (Czech Republic).‏
43- Turnbull, K.M., Marion, D. Gaborit, T. Appels, R. and Rahman. S. (2002) Temporal expression of grain hardness in the developing wheat endosperm. Planta 216: 699–706.
44- Ullison E. A. (1987) Air-dry Energy Feeds. Feeds and Feeding, 2nd Edition. Reston Publishing Company Inc, pp. 161 – 163.
45- Vaugelade, P., Posho, L. Darcy-Vrillon, B. Bernard, F. Morel, M.T. and Duee. P.H. (1994) Intestinal oxygen uptake and glucose metabolism during nutrient absorption in the pig. Proc. Soc. Exp. Biol. Med. 207: 309–316.
46- Viveros, A., Brenes, A. Pizarro, M. Castano. M. (1994) Effect of enzyme supplementation of a diet based on barley, and autoclave treatment, on apparent digestibility, growth performance and gut morphology of broilers. Anim. Feed Sci. Technol. 48, 237-251.
47- Weurding, R. E., Veldman, A. Veen, W. A. G. Vander, P. J. and Verstegen. M. W. A. (2001) Starch digestion rate in the small intestine of broiler chickens differs among feedstuffs. J. Nutr. 131:2329–2335 .