شناسائی ملکولی گونه های بیماریزای آناپلاسما در گوسفندان استان مازندران

نوع مقاله : مقاله کامل

نویسندگان

1 استادیار، بخش تحقیقات بیماری‌های انگلی، موسسه تحقیقات واکسن و سرم‌سازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

2 دانشیار، بخش تحقیقات بیماری‌های انگلی، موسسه تحقیقات واکسن و سرم‌سازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

چکیده

هدف از این مطالعه تعیین گونه های آناپلاسما در گوسفندان استان مازندران بود. برای این منظور تعداد 95 نمونه خون از طریق رگ وداج گوسفند بطور تصادفی از نقاط مختلف استان مازندران جمع آوری گردید. در ابتدا DNA استخراجی از نمونه های خونی با جفت آغازگری که قطعه 1468 جفت بازی از ژن 16S rRNA جنس آناپلاسما را تکثیر می کرد، تکثیر شد. سپس جهت افزایش حساسیت آزمون، محصول PCR اولیه با جفت آغازگری که قطعه 345 جفت بازی از ژن 16S rRNA جنس آناپلاسما را تکثیر می کرد، تکثیر شد. 70 نمونه(7/73%) از 95 نمونه اخذ شده در اولین PCR و nested-PCR از نظر جنس آناپلاسما مثبت شدند. تمامی نمونه های مثبت با nested-PCR اختصاصی از نظر وجود آناپلاسما فاگوسیتوفیلم بررسی شدند و22 نمونه(2/23 %) از نظر آناپلاسما فاگوسیتوفیلم مثبت تشخیص داده شد. DNA استخراجی نمونه های مثبت با استفاده از جفت آغاز گرهای اختصاصی که قطعه 866 جفت بازی از ژن msp4 آناپلاسما اوویس را تکثیر می کرد، تکثیر شد. 56 نمونه(9/58%) از95 نمونه از نظر آناپلاسما اوویس مثبت بودند. این مطالعه اولین تشخیص ملکولی گونه های آناپلاسما در گوسفندان در استان مازندران است. نتایج نشان می دهد که در بین درصد آلودگی گوسفندان به آناپلاسمافاگوسیتوفیلوم، در فصول مختلف سال اختلاف معنی دار وجود دارد (05 /0 P<). همچنین از نظر آلودگی به آناپلاسما اوویس، از میان متغییر های مورد بررسی، بین فصول مختلف، ارتفاع از سطح دریا، جنس و طول جغرافیائی، اختلاف معنی دار وجود دارد (05/0 P<).

کلیدواژه‌ها

عنوان مقاله [English]

Molecular Identification of Anaplasma pathogenic Species in Sheep in Mazandaran Province

نویسندگان [English]

  • N. Vahedi Noori 1
  • V. Noaman 2
1 Animal Parasitic Disease Research Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
2 Animal Parasitic Disease Research Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
چکیده [English]

The aim of this study was to determinate the variety of Anaplasma species among sheep of Mazandaran province, Iran .For this purpose, a total of 95 blood samples were collected via the jugular vein from healthy sheep randomly.The extracted DNA from blood cells were amplified by Anaplasma-all primers, which amplify an approximately 1468bp DNA fragment from region of 16S rRNA gene from various members of the genus Anaplasma. 70 (73.7%) out of 95 sheep blood samples were Anaplasma spp. positive by first PCR and nested PCR. All sheep positive samples were analysed for the presence of A. phagocytophilum and 22 of sheep blood samples (23.2%) were positive for A.phagocytophilum. The extracted DNA from positive Anaplasma spp. samples were amplified by Anaplasma ovis specific primers, which amplify an approximately 866bp DNA fragment from region of msp4 gene. 56 out of 95 sheep blood samples (58.9%) were positive for Anaplasma ovis. This study is the first molecular detection of Anaplasma species from sheep in Mazandaran province. The results show that there is a significant difference among the percentage of sheep infection to Anaplasma phagocytophilum with different seasons of the year (P<0.05).Also, there are a significant difference among the percentage of sheep infection to anaplasma ovis with different seasons of the year, above sea level, Sex and longitude. (P<0.05).

کلیدواژه‌ها [English]

  • Molecular
  • Anaplasma Species
  • Sheep
  • Mazandaran

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  • تاریخ دریافت: 26 آبان 1397
  • تاریخ بازنگری: 08 اردیبهشت 1398
  • تاریخ پذیرش: 08 اردیبهشت 1398

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