بررسی کارآیی واکسن زنده تخفیف حدت یافته برونشیت عفونی سویه B08IR/ا793 ساخت موسسه رازی

نوع مقاله: مقاله کامل

نویسندگان

1 استادیار بخش تحقیق و تولید واکسن های ویروسی طیور، موسسه تحقیقات واکسن و سرم سازی رازی، کرج، ایران

2 دانشیار بخش تحقیق و تولید واکسن های ویروسی طیور، موسسه تحقیقات واکسن و سرم سازی رازی، کرج، ایران

چکیده

از واکسن‌هایی که در حال حاضر برای کنترل بیماری برونشیت عفونی طیور مورد استفاده قرار می‌گیرد، واکسن حاوی سویه i793/Bمی‌باشد. بدنبال اثبات حضور ویروس i793/B در اکثر مرغداری‌های کشور واکسن زنده تخفیف حدت‌یافته برونشیت عفونی سویه i793/B081R، در موسسه رازی تولید شد. هدف از انجام این پروژه ارزیابی کارآیی واکسن i793/B081Rبر اساس پروتکل OIE با استفاده از ارزیابی فعالیت مژک‌های نای (Ciliary activity) پرندگان مایه‌کوبی شده بعد از چالش با ویروس حاد بود. نتایج بررسی حرکت مژک‌های نای پس از چالش با ویروس حاد در گروه‌های رازی، وارداتی و شاهد بترتیب 5/1، 7/1 و 4 بود. طبق استاندارد امتیاز کمتر یا مساوی 2 نشان‌دهنده مصونیت پرنده مایه‌کوبی شده در مقابل ویروس حاد می‌باشد و امتیاز بیشتر از 2 نشان‌دهنده عدم مقاومت پرنده مایه‌کوبی شده است. نتایج بدست‌آمده موید این نکته بود که واکسن رازی در مقایسه با واکسن وارداتی از کارآیی بالایی برخوردار است و 100 درصد پرندگان مایه‌کوبی شده در مقابل ویروس حاد مقاومت نمودند درحالی‌که تمامی پرندگان گروه شاهد توقف کامل مژک‌های نای را بدون هیچ‌گونه مقاومت در برابر ویروس حاد نشان دادند. همچنین آزمایشات سرولوژی (ELISA) و نوترالیزاسیون سرم (SN (Serume Neutralization افزایش قابل توجه تیتر سرمی در مقایسه با گروه شاهد نشان دادند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Efficacy evaluation of Razi Infectious Bronchitis Live Attenuated Vaccine 793/B08IR Strain

نویسندگان [English]

  • B. Khalesi 1
  • Sh. Masoudi 2
  • M.M. Ebrahimi 1
  • L. Pishraft-Sabet 1
1 Assistant Professor, Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.
2 Associate Professor,Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.
چکیده [English]

Vaccines that are currently used to control infectious bronchitis are vaccines containing 793/B strain. Following the evidence of 793/B viral infection in most of the country's poultry farms, a live vaccine for virulent infectious bronchitis strain 793/B08IR was developed at the Razi Institute. The purpose of this project was to evaluate the efficacy of vaccine 793/B08IR based on the OIE protocol by assessing the activity of ciliary activity of IBV vaccinated birds after the challenge of acute viruses. The results of the study of trachea after the challenge of acute virus in the Razi, imported and control groups were 1.5, 1.7 and 4, respectively. According to the standard, a score of less than or equal to 2 indicates the immunity of the bird vaccinated against the acute virus, and a score of more than 2 indicates the lack of resistance of the bird. The results indicated that Razi vaccine was highly effective compared to the imported vaccine, and 100% of the birds vaccinated against the acute virus resisted, while all the birds in the control group stopped full trachea without any resistance acute virus showed. Serological tests (ELISA) and serum neutralization (SN) also showed a significant increase in serum titer compared to the control group.

کلیدواژه‌ها [English]

  • vaccine
  • Infectious bronchitis 793/B Serotype
  • Cilliostasis

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