عنوان مقاله [English]
Mastitis is one of the major diseases of dairy cows throughout the world which is associated with severe economic losses for dairy producer. Antimicrobial resistance, production of secondary harmful metabolites and drug residues in animal products following use of antibiotics are shifting interests of clinicians into the use of medical plants. The aim of present study was to evaluate in-vitro antibacterial activity of Achillea essential oil extract against some gram positive bacteria isolated from cases of bovine mastitis in Shahrekord. Essential oil was obtained by Clevenger apparatus according to British pharmacopeia protocol. The chemical composition was analyzed by GC/MS. Disk diffusion and macro dilution methods were used for the determination of antibacterial activity. Disk diffusion method showed that the essential oil in concentrations of 5mg/mL may inhibit all bacterial growth. Macro dilution test revealed that for Streptococcus agalactiae, Streptococcus dysgalactiae, and Enterococcus faecalis, the MIC and MBC were 4.73 mg/mL and 9.46 mg/mL, respectively. The MIC and MBC of Staphylococcus aureus were 4.73 mg/mL and 2.365mg/mL, respectively. According to the results, it was concluded that essential oil of Achillea extract might be used in vitro as a bactericidal agent for the bacteria that cause mastitis.
1. Arias, C., Singh, K., Panesso, D., Murray, BE. (2007). Time-Kill and Synergism Studies of Ceftobiprole against Enterococcus faecalis, Including ß-Lactamase-Producing and Vancomycin-Resistant Isolates. International Journal of Antimicrobial Agents, 51(6):2043-2047.
2. Ataei, Z., Abdolahi, H., Naderipour, S., Siros, Mohamadi. (2006). An in vitro study of the effects of Yarrow, Chamomile and Rhubarb herbal extracts on Candida albicans and common oral bacteria. Journal of the Indian Dental Association. 18(3): 0-0.
3. Barel, S., Segal, R., Yashphe, J.(1991). The antimicrobial activity of the essential oil from Achillea fragrantissima. Journal of Ethnopharmacology, 33(1): 187-191.
4. Baser,KH., Demirci, B., Demirci, F., Koçak, S., Akinci, C., Malyer, H., Güleryüz, G. (2002). Composition and antimicrobial activity of the essential oil of Achillea multifida. Planta Medica. 68(10): 941-943.
5. Candan, F., Unlu, M., Tepe, B. (2003). Antioxidant and antimicrobial activity of the essential oil and methanol extracts of Achillea millefolium subsp. millefolium Afan. Journal of Ethnopharmacology. 87(2): 215-220.
6. Chung, KT., Thomasson, WR., Wu-Yuan, CD. (1990). Growth inhibition of selected food-borne bacteria, particularly Listeria monocytogenes, by plant extracts. Journal of Applied Bacteriology. 69(4): 498-503.
7. Cristina, A., Figueiredo, S., Salomé, M., Pais, S. (1991). Achillea millefolium (yarrow) cell suspension cultures: Establishment and growth conditions. Biotechnology Letters. 69(4): 498-503.
8. Foster, S., Duke, J.A. (1990). A field guide to medicinal plants. (2nd th) Eastern and Central N America. Houghton Mifflin Co. p: 225-227.
9. Hesanpour, A.k., Amini, F. (2003). Cell Culture and Plant Tissue. (3nded). Isfahan. Jahad Publications. p. 150-181.
10. Lasagno MC, Vissio C, Reinoso EB., Reinoso, E.B., Raspanti, C., Yaciuk, R., Larriestra, A.J., et al. (2012). Development of an experimentally induced Sreptococcus uberis subclinical mastitis in goats. Veterinary Microbiology. 154(3): 376-383.
11. Morteza-Semnani, K., Azadbakht, M., Khansari, N. (2003). The essential oils composition of Achillea wilhelmsii C. Koch leaves and flowers. Journal of Medicinal Plants Research, 2: 55-58.
12. Mosaferi, S. (2003). Mammary disease in domestic animals. (1nd ed). Tabriz. Islamic Azad University of Tabriz Publications. P:35-38.
13. Nourmohammadzadeh, F. (2004). Cattle Diseases. (2nd ed). Tehran. Noor Bakhsh Publications. P:38-40.
14. Quinn P.J., Carter, M.E., Markey, B.K., Leonard, F.c., Hartigan, p., Faninig, S., Fitz Patrick E.S. (1994) Clinical Veterinary Microbiology. (1nd ed). London. mosby London: p: 118-126.
15. Radostits, OM., Gay, C.C, Hinchcliff, K.W. (2006). Veterinary medicine. (10th ed). London, Saunders Elsevier. 5: 673-762.
16. Rasooli, I., Rezaei, M.B. (2002). Bioactivity and chemical properties of essential oils from Zataria multiflora and Mentha longifolia. Iranian Journal of Parasitology. 14(2): 141-146.
17. Saeidnia, S., Gohari, A.R., Yassa, N. (2005). Composition of the volatile oil of Achillea conferta from Iran. Daru. 13(1). 34-36.
18. Shrmn, S.N., Adlakha, S.C. (1997). Text book of veterinary microbiology. (1nd ed). Indian. Vikas Publishing House. P:152-178
19. Stojanovic, G., Radulovic, N., Hashimoto, T. (2005). In vitro antimicrobial activity of extracts of four Achillea species: The composition of Achillea clavennae L. (Asteraceae) extract. Journal of Ethnopharmacology, 101(1): 185-190.
20. Tajik, H. (2009). Comparative evaluation of antimicrobial efficacy of aqueous and alcoholic extracts of yarrow against pathogenic microorganisms. Urmia Medical Journal. 4(2): 114-122.
21. Tajik, T., Shokouhi, Sabet., Jalali, F., Sobhani, A., Shahbazi, Y., Soleimanzadeh, M. (2008). In vitro assessment of antimicrobial efficacy of alcoholic extract of Achillea millefolium in comparison with Penicillin derivatives. Journal of Animal and Veterinary Advances. 7(4): 508-11.