عنوان مقاله [English]
The aim of this study was to investigate the effect of Naloxone on histomorphometrical change of testis induced by morphine administration in male wistar rats. Fifteen male wistar rats divided into three groups : 1- control group. 2- Morphine experimental group (IP received 5 mg/kg morphine for 30 days). 3- Morphine-naloxone experimental group (IP received 5 mg/kg morphine for 30 days and one dose IP received 2mg/kg Naloxone at 30th day). Ten days after the end of experiment the specimens were obtained at 40th . Results showed that the thickness of the wall of seminiferous tubule, and the weight of testis, in morphine experimental group significantly decreased (p ≤ 0.05). Also, the number of the spermatogenic cells in the seminiferous tubule were decreased in morphine experimental group. In addition, the thickness of the wall of seminiferous tubule, the weight of testis and the number of the spermatogenic cells were increased in morphine-naloxone experimental group compared with morphine experimental group but the number of the spermatogenic cells increased in morphine-naloxone experimental group with no significant difference in comparison with morphine experimental group (0.05 ≤ P). It is concluded that morphine decreased the sexual activity, the number of spermatogenic cells, the thickness of seminiferous tubule, and the weight of testis but Naloxone can improve this decrement.
1-Bakker, J. and Baum, M. (2000). Effect of gonadal steroids on pituitary LH secretion, and medio basal hypothalamic GnRH mRNA inferrets. J Reprod Fertility, Vol, 205.pp: 315-321.
2-Bakker, J. and Baum, M. (2000). Neuroendocrine regulation of GnRH release induced ovulators. Front in Neuroendocrinology, Vol, 21. pp: 220-262.
3-Daniell, HW. (2002). Hypogonadism in men consuming sus tianed – action of opioids. J pain, Vol,3, No, 5. pp: 377-384.
4-Ganji, LVP. Bannoth, RN. Jasmine, EKo. and Ganji, PN.(2014). Effects of naloxone, serotonin, and dopamine on reproduction of the freshwater crab Barytelphusa guerini, J Exp Zool, Vol, 321, No, 3. pp: 173-182.
5-Kest, B. and Hopkins, E. (2001). Morphine tolerance after chronic intra cerebroventricular injection in male and Female mice. Br Res, Vol, 892. pp: 208 -210.
6-Kilgour, RJ. Pisselet, C. Dubois, MP. and Courot, M. (1998). Ramlamds need FSH, LH for normal testiculae growth, sertoli cell number and onset of spermatogenesis. Reprod Nutr Dev, Vol,38,No, 5. pp: 539-550.
7-Khaki, A. Fathizad, F. Nouri, M. Khaki, AA. Ozanci, C. Ghafari-novin, M. and Hamedeh, M. (2009). The effects of Ginger on spermatogenesis and sperm parameters of rat. Iranian Journal of reproductive Medicine, Vol, 7, No, 1. pp: 7-12.
8-Krause, W. (1976). Prolactin in male reproduction. Hautarzt, Vol, 27, No, 9. pp: 416-421.
9-Laxmi, M. and Vijayan, E. (1988). Effect of chronic systemic administration of opioid peptide, Naloxone and N-acetylendrophin antiserum on gonadotropins and testicular Functions in the rat. Indian J EXP Biolo, Vol, 36. pp: 255-261.
10-Mendelson, JH. Mendelson, JE. and Patch, VD. (1975). Plasma testosterone in herion addiction and during methadone maintenance. J pharmacol Exp Ther, Vol,192, No, 1. pp: 211-217.
11-Rangi, G. De Lauretis, L. and Betetti, O. (1988). Gonadal function in male herion and methadone addiets. Int J Androl, Vol, 11, No, 2. pp: 93-100.
12-Sepehri, GH. Golamhosseinian, A. Hejazian, H. Dabiri, SH. and Atapour, F. (1995). The effects of opium addiction on testosterone and gonado tropins in addicted men. Journal of Kerman University of Medical Sciences, Vol, 2, No, 2. pp: 56-60.
13-Shahramian, I. Kohan, F. and Moradi, A. (2004). Opioids in pediatric. Opioid history, first edition. Golban press, 1-3, 80.
14-Thomas, JA. and Domborsky, JT. (1975). Effects of methadone on the male reproductive system. Arch Int pharmgyn Ther, Vol,215, No, 2. pp: 215-221.
15-Yilmaz, B. konar, V. and sandal, S. (2003). Influnce of chronic morphine Exposure on serum LH. FSH, testosterone levels in the developing male rats. Physiol Behav, Vol, 282. pp: 189 -196.