کارایی روش های مختلف تجویز زودهنگام پروبیوتیک بر کاهش استقرار سالمونلا در بلدرچین ژاپنی

نویسندگان

1 دانش آموخته گروه پرورش و تولید طیور، دانشکده کشاورزی، دانشگاه تربیت مدرس

2 استادیار گروه پرورش و تولید طیور، دانشکده کشاورزی، دانشگاه تربیت مدرس

چکیده

      تعداد 270 عدد تخم نطفه دار بلدرچین در 9 گروه 30 تایی در دستگاه جوجه کشی خوابانیده شدند. گروه های آزمایشی شامل: شاهد منفی (بدون چالش با سالمونلا)، شاهد (چالش با سالمونلا) و گروه های دریافت کننده پروبیوتیک (تزریق به تخم، افشانه بر روی جوجه یک روزه، گاواژ دهانی، تلقیح به کلوآک و سه گروه تلفیقی: تلقیح به کلوآک و تزریق به تخم، تلقیح به کلوآک و افشانه و تلقیح به کلواک و گاواژ دهانی) بودند. به هر گروه 15 قطعه جوجه اختصاص داده شد. جوجه های تمامی گروه های آزمایشی به جز گروه شاهد منفی، 36 ساعت پس از دریافت پروبیوتیک، با 104×75/1 واحد تشکیل دهنده کلنی در میلی لیتر کشت تازه سالمونلا انتریتیدیس از راه دهان چالش داده شدند. بررسی جای گیری سالمونلا در محل لوزه های سکومی، یک روز پس از چالش، با استفاده از محیط های کشت غنی کننده و اختصاصی سالمونلا بر روی 12 قطعه جوجه بلدرچین انجام شد. در سن 8 روزگی، وزن بدن و اندام های بورس فابریسیوس، طحال و جگر ثبت گردید. کارایی روش های مختلف تجویز زودهنگام پروبیوتیک بر جای گیری سالمونلا در دستگاه گوارش بلدرچین معنی دار بود (01/0 >P). تنها کاربرد پروبیوتیک به روش افشانه کاهش فاحشی در استقرار سالمونلا در مقایسه با سایر روش های تجویز پروبیوتیک داشت. بالاترین وزن بدن در گروه دریافت پروبیوتیک از راه دهان مشاهده شد (05/0 >P). روش تجویز پروبیوتیک بر وزن نسبی جگر و بورس تاثیر معنی داری داشت (05/0 >P).

عنوان مقاله [English]

Efficiency of early (in hatchery) probiotic administration methods against salmonella colonization in Japanese quail

نویسندگان [English]

  • K Seifi 1
  • M.A Karimi Torshizi 2
1 Graduated from Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University
2 Assistant Professor, Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University.
چکیده [English]

Total of 270 quail fertile eggs were set in hatchery. Experimental groups including: Negative control (neither received probiotic treatment nor challenged by Salmonella), positive control (challenged by Salmonella but not probiotic treatment) and 7 in hatchery administration methods: in ovo injection, spray, oral gavage, cloacal drinking (cloacal inoculation), cloacal drinking – in ovo injection, cloacal drinking– spray and cloacal drinking – oral gavage. Each group included 15 day old chicks. All chicks were challenged by 1.75104 cfu / ml Salmonella eneteritidis using oral gavage except negative control 36 h post probiotic treatment. 12 chicks from each group were euthanized by CO2 inhalation 24 h postchallange. Cecal tonsil aseptically collected and colonisation of Salmonella were probed using culture technique. The spray method significantly reduced Salmonella enteritidis colonization (P<0.01). On 8th day higher BW were observed in oral group (P<0.05). The administration methods were significantly effective on the liver and bursa of Fabricius weight (P<0.05). In conclusion probiotic administration using spray on quails day old chicks were the most effective in prevention of Salmonella colonization.

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