اثر استفاده از بنتونیت سدیم و دیواره سلولی مخمر بر میزان تولید پادتن در پاسخ به واکسیناسیون علیه بیماری بورس عفونی درجوجه‌های گوشتی مبتلا به آفلاتوکسیکوزیس تجربی

نویسندگان

1 استادیار دانشکده دامپزشکی دانشگاه آزاد اسلامی واحد ارومیه، ارومیه

2 دانش آموخته دکترای دامپزشکی، دانشکده دامپزشکی، دانشگاه آزاد اسلامی واحد ارومیه، ارومیه

3 دکترای دامپزشکی و کارشناس مرکز تحقیقات مرغ بومی جهاد کشاورزی استان آذربایجان غربی

چکیده

آفلاتوکسین اثرات مضر بر عملکرد و ایمنی در برابر بیماری ها دارد که بر این اساس مواد مختلف جاذب سموم قارچی جهت کاهش اثرات سوء آفلاتوکسین استفاده می گردد. در این مطالعه توانایی دیواره‌ی سلولی مخمر و بنتونیت سدیم به عنوان مواد جاذب سموم قارچی در کاهش مسمومیت با آفلاتوکسین در جوجه‌های گوشتی تغذیه شده از جیره‌ی حاوی ذرت کپک‌زده و حاوی آفلاتوکسین بررسی گردید. 240 قطعه جوجه گوشتی از سویه راس 308 در قالب طرح کاملاً تصادفی با 10 گروه آزمایشی و 3 تکرار و جمعاً در 30 واحد آزمایشی از نظر تولید پادتن بر علیه بیماری بورس عفونی بررسی شد. تیمار های آزمایشی شامل: جیره شاهد فاقد آفلاتوکسین، جیره شاهد حاوی آفلاتوکسین، جیره های حاوی آفلاتوکسین و دو سطح مختلف دیواره سلولی مخمر (5/0 و 1 درصد) و دو سطح بنتونیت سدیم(5/0 و 1 درصد) و جیره حاوی آفلاتوکسین با سطوح ترکیبی این دو ماده بود. آزمایش از سن 8 روزگی شروع و در سنین 21، 28، 35 و 42 روزگی با اخذ نمونه خونی، میزان پادتن به روش الایزا اندازه گیری گردید. دیواره سلولی مخمر در بهبود میزان تیتر پادتن، بهتر از بنتونیت سدیم عمل کرده بود. ترکیب این مواد (1/0 درصد دیواره سلولی مخمر و 5/0 درصد بنتونیت سدیم) در جیره های حاوی آفلاتوکسین سبب کاهش معنی داری در تاثیر آفلاتوکسین بر تولید پادتن برعلیه بیماری بورس عفونی شده است، بطوریکه جوجه های تغذیه شده با جیره حاوی 5/0 در صد بنتونیت سدیم و 05/0 درصد دیواره سلولی مخمر، یا جیره حاوی 5/0 در صد بنتونیت سدیم و 1/0 در صد دیواره سلولی مخمر در مقایسه با تیمارهای دیگر تیتر پادتن بالایی داشتند.

عنوان مقاله [English]

Efficacy of sodium bentonite and esterified glucomannan on antibody titer in response to infection bursal disease vaccine during experimental Aflatoxicosis in broiler

نویسندگان [English]

  • H Ghahri 1
  • M Abdollahfam 2
  • S.J Koohkamari 3
1 Assistant Professor of Islamic Azad University Urmieh Branch
2 Graduated of Veterinary Medicine, Islamic Azad University Urmieh Branch
3 Expert of Poultry Research Center of West Azarbayjan
چکیده [English]

Studies have shown that aflatoxin is immunosuppressive and its ingestion in feed has resulted in decreased immunity in vaccinated birds .Therefore, the purpose of the present study was to determine the efficacy of sodium bentonite (SB) and esterified glucomannan (E-GM) of yeast cell wall, in counteracting the toxic effects of aflatoxin (AF) on immunization against Infectious Bursal Disease (IBD) in broiler feed with naturally contaminated diet with aflatoxin. Seven-day-old chicks were randomly assigned to 10 dietary treatments in three replicates of 8 chicks. Treatments were:1) control; 2) naturally contaminated diet; 3, and 4) naturally contaminated diet (NCD) supplemented with 0.5 and 1.0% SB respectively; 5 and 6) (NCD) with 0.05 and 0.1% E-GM respectively; 7) NCD supplemented with 0.5% SB and 0.05 % E-GM, 8) NCD supplemented with 0.5% SB and 1.0 % E-GM, 9) NCD supplemented with 1.0% SB and 0.05 E-GM, and 10) NCD supplemented with 1.0% SB and 0.1% E-GM. blood samples were taken in 21, 28, 35 and 42 day of age and the titers of antibody against IBD were measured by ELISA test. The addition of E-GM and SB to the aflatoxincontaining diet ameliorated the adverse effects of aflatoxin on IBD antibody titers, but E-GM supplementation to the contaminated diet with aflatoxin proved to be much more effective in the amelioration of the adverse effect of aflatoxin on homoral immunity against IBD. These results clearly demonstrated that simultaneous addition of E-GM and SB (Treatment 8) to the AF-containing diet provided significant reduction on the immunotoxic effects of AF.

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