جستجوی monocytogenes Listeria در موارد آندومتریت گاوهای شهرکرد به روش PCR

نویسندگان

1 استادیار گروه علوم درمانگاهی دانشکده دامپزشکی و عضو پژوهشکده بیماری های مشترک انسان و دام دانشگاه شهرکرد

2 دانشجوی دانشکده دامپزشکی و عضو پژوهشکده بیماری های مشترک انسان و دام دانشگاه شهرکرد

3 دانشجوی دانشکده دامپزشکی دانشگاه شهرکرد

4 استادیار گروه مامایی و بیماری های تولیدمثل دام دانشکده دامپزشکی دانشگاه آزاد اسلامی واحد شهرکرد

چکیده

آندومتریت به عنوان یک ریسک فاکتور در ایجاد کیست تخمدانی، آنتروس و سایر مشکلات تناسلی مطرح می باشد و با اثر گذاری بر تولید شیر، به زیان های جدی اقتصادی منجر می شود.L. monocytogenes می‌تواند منجر به ایجاد آندومتریت گردد. با توجه به اهمیت آنچه ذکر گردید، مطالعة حاضر جهت ردیابی L. monocytogenes در موارد آندومتریت گاو در شهرکرد انجام شد. مجموعاً 180 نمونه از ترشحات رحمی به وسیلة سواب استریل از موارد آندومتریت گاو از شهرکرد جمع آوری شد. سواب ها مستقیماً درون  enrichment broth Listeria قرار داده شدند. سپس نمونه های غنی شده بر روی محیط Palcam agar به شکل خطی کشت داده شدند. کلنی های مشکوک به وسیله آزمون واکنش زنجیره ای پلی مراز (PCR) مورد ارزیابی قرار گرفتند. هیچ نمونة آلوده به L. monocytogenes یافت نشد. مطالعة حاضر نشان دهندة عدم حضور
L. monocytogenes در موارد آندومتریت گاو در این منطقه است. 
 

عنوان مقاله [English]

PCR detection of Listeria monocytogenes in bovine endometritis from Shahrekord, Iran

نویسندگان [English]

  • A Jafari Dehkordi 1
  • H Tahmasby 2
  • A Momeni 3
  • T Taktaz Hafshejani 4
1 Assistant Professor, Department of Clinical Science, Faculty of Veterinary Medicine and Member of Research Institute of Zoonotic Diseases, Shahrekord University, Shahrekord, Iran
2 Student, Faculty of Veterinary Medicine and Member of Research Institute of Zoonotic Diseases, University of Shahrekord, Shahrekord, Iran
3 Student, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran
4 Assistant Professor, Department of Veterinary Reproduction and Obstetrics, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
چکیده [English]

Endometritis as a risk factor for cystic ovarian disease, anoestrus and other reproductive disorder, is characterized by impact of dairy production performance and causes serious economic losses. Listeria monocytogenes can cause endometritis. Considering the importance of what mentioned present study was conducted to detection of L. monocytogenes in bovine endometritis from Shahrekord, Iran. Altogether 180 vaginal discharge samples of bovine endometritis were collected with sterile cotton swabs from Shahrekord, Iran. The swabs were placed directly into Listeria enrichment broth. Secondly enrichments were streaked on Palcam agar. Suspected colonies were evaluated for detecting L. monocytogenes by polymerase chain reaction (PCR) method. L. monocytogenes was not found in any samples. Present study suggests absence of L. monocytogenes in bovine endometritis in the region.

1- Bicalho, R.C., Machado, V.S., Bicalho, M.L., Gilbert, R.O., Teixeira, A.G., Caixeta, L.S. and Pereira, R.V. (2010) Molecular and epidemiological characterization of bovine intrauterine Escherichia coli, Journal of Dairy Science. 93, 5818-5830.##

2- Chaudhari, S.P., Malik, S.V., Chatlod, L.R. and Barbuddhe, S.B. (2004) Isolation of pathogenic Listeria monocytogenes and detection of antibodies against phosphatidylinositol-specific phospholipase C in buffaloes, Comparative Immunology, Microbiology & Infectious Diseases. 27(2):141-8.##

3- Coleman, D.A., Thayne, W.V. and Dailey, R.A. (1985) Factors affecting reproductive performance of dairy cows, Journal of Dairy Science. 68, 1793-1803.##

4- Choi, W.S. and Hong, C. (2003) Rapid enumeration of Listeria monocytogenes in milk using competitive PCR, International Journal of Food Microbiology. 84 ,79– 85.##

5- Dubuc, J., Duffield, T.F., Leslie, K.E., Walton, J.S. and Leblanc, S.J. (2011) Effects of postpartum uterine diseases on milk production and culling in dairy cows, Journal of Dairy Science. 94, 1339-1346.##

6- Griffin, J.F., Hartigan, T.P. and Nunn, W.R. (1974) Nonspecific uterine infection and bovine fertility. I. Infection patterns and endometritis during the first seven weeks post-partum, Theriogenology. 3, 91-106.##

7- Ramisse, J., Lepareur, F., Pourdelet, E., Poudelet, M. and Priouzeau, M. (1986) Epidemiological survey of non-Brucella abortion among cows in Vendee, France, Point-Veterinaire. 18, 81–88.##

8- Ruder, C.A., Sasser, R.G., Williams, R.J., Ely, J.K., Bull, R.C. and Butler, J.E. (1981) Uterine infection in the postpartum cow. II. Possible synergistic effect of Fusobacterium necrophom and Corynebacterium pyogenes, Theriogenology. 66, 573-580.##

9- Shakuntala, I., Malik, S.V., Barbuddhe, S.B. and Rawool, D.B. (2006) Isolation of Listeria monocytogenes from buffaloes with reproductive disorders and its confirmation by polymerase chain reaction, Veterinary Microbiology. 117, 229-234.##

10- Sharda, R., Moghe, M.N. and Tanwani, S.K. (1991) Antibiotic sensitivity pattern of bacteria isolated from repeat breeding animals, Indian Veterinary Journal. 68, 197–200.##

11- Sharma, M., Batta, M.K., Katoch, R.C., Asrani, R.K., Nagal, K.B. and Joshi, V.B. (1996) Listeria monocytogenes abortions among migratory sheep and goats in Himachal Pradesh, Indian Journal of Animal Sciences. 66, 1117–9.##

12- Sharma, M., Batta, M.K., Katoch, R.C. and Andersen, A.A. (2008) A field investigation of bacterial etiology of abortions among migratory sheep and goats in North-West hill states of India, Veterinarski Arhiv. 78 (1), 65-71##

13- Thakur, S. (2000) Studies on Listeric infections in organized farms. MVSc Thesis, Indian Veterinary Research Institute, Izatnagar, India.##

14- Yadav, M.M. and Roy, A. (2009) Prevalence of Listeria spp including Listeria monocytogenes from apparently healthy sheep of Gujarat State, India, Zoonoses Public Health. 56(9-10), 515-24.##