مطالعه بافت شناسی لوله رحمی گاومیش بالغ رودخانه ای در طول دوره استروس

نویسندگان

1 گروه علوم پایه، دانشکده دامپزشکی دانشگاه ارومیه

2 دانش آموخته دانشکده دامپزشکی، دانشگاه ارومیه

3 سازمان تحقیقات و آموزش کشاورزی، تهران

چکیده

جهت مطالعه بافت شناسی لوله رحمی گاومیش تعداد 10 دستگاه تناسلی گاو میش بالغ نمونه برداری شد. مطالعه عمومی بافت شناسی با روش هماتوکسیلین- ائوزین انجام شد و روش های رنگ آمیزی اختصاصی به کار گرفته شده به ترتیب عبارت بودند از: ورهاف، تولوئیدین بلو، و PAS. این مطالعه نشان داد که رشته‌های الاستیک در بافت همبند چین‌ها و دیواره لوله رحمی و طبقه عضلانی و همچنین به میزان کم در طبقه سروزی وجود دارد. در مرحله لوتئال بافت پوششی لوله رحمی مخصوصاً در ناحیه آمپول محتوی ترشحات حاوی گلیکوپروتئین یا کربوهیدرات می‌باشد. این نتایج اهمیت ناحیه آمپول را در تغذیه جنین نشان می دهد. این مطالعه نشان داد که ماست‌سل‌ها به تعداد بسیار کم در بافت همبند حضور دارند. دانه‌های متاکروماتیک شبیه ماست‌سل‌ها در سیتوپلاسم سلول‌های پوششی ترشحی اویدکت در مرحله لوتئال مشاهده شد. 

عنوان مقاله [English]

Histological study of uterine tube in adult river buffalo during estrus cycle

نویسندگان [English]

  • R Shahrooz 1
  • A Rahmani 2
  • F Nabizadeh 3
1 Department of Anatomical Sciences, School of Veterinary Medicine, Urmia University, Urmia, Iran.
2 Graduated from School of Veterinary Medicine, Urmia University, Urmia, Iran
3 Agricaltural Research and Extention Education Organization, Tehran, Iran.
چکیده [English]

For histological study of buffalo's uterine tube, 10 genital organs of adult buffalos were sampled. General histology was taken out by H & E method, and special staining methods that used were respectively Verhoeff, toluidine blue, and PAS. This study revealed that elastic fibers exist in connective tissue of folds and wall of uterine tube and tunica muscularis, and also they exist relatively less in tunica serosa. In luteal phase, epithelium of uterine tube particularly in ampulla contains glycoprotein or carbohydrate secretion. These results revealed the importance of ampulla for primary nutrition of embryo. This study revealed that mast cells were scanty in connective tissue. Mast cell like metachromatic granules observed in cytoplasm of epithelial secretory cells of oviduct in luteal phase.

1- Areekijseree, M., & Vejaratpimol, R. ( 2006) In vivo and in vitro study of porcine oviductal epithelial cells, cumulus oocyte complexes and granulosa cells: A scanning electron microscopy and inverted microscopy study. Micron. 37(8):707-16.##

2- Ayen, E., Shahrooz, R., and Kazemie, S.(2002) Histological and histomorphometrical changes of different regions of oviduct during follicular and luteal phases of estrus cycle in adult Azarbaijan buffalo. Iranian Journal of Veterinary Research, V.13 (1), Ser. 38: 42-48##

3- Ball, B. A., Dobrinski, I., Fagnan, M. S., Thomas, P. G. A. (1997) Distribution of Glycocojugates in the uterine tube of horses. American Journal of Veterinary Reserch, 58(8): 816-822.##

4- Banks, W. J. (1993) Applied Veterinary Histology, 3rd ed., (Mosby Year Book, London). pp.: 452-456.##

5- Ellington, J. E., Evenson, D. P., Fleming, J. E., Brisbois, R. S., Hiss, G. A., Broder, S. J., Wright, R. W. ( 1998) Coculture of human sperm with bovine oviduct epithelial cells decreases sperm chromatin structural changes seen during culture in media alone. Fertil Steril. 69(4):643-9.##

6- Eurell, J. A., and Frappier, B. L. (2006) Dellmann’s Text Book of Veterinary Histology, 6th ed., (Blacwell publishing USA). pp.: 256-278.##

7- Faundes, R., Duszewka, A. M., sport, I., Sttarska, E. (1974) Occureeence of leukocyts and epithelial cells in the lumen of the reproductive tract during the ovarian cycle. In proceding 18th world Buiatrics Congress: 26 Congress of the Italian.##

8- Faussone-Pellegrini, M. S., & Bani, G.( 1990) The muscle coat morphology of the mouse oviduct during the estrous cycle. Arch Histol Cytol. 53(2):167-78. ##

9- Gretchenl, H. L. (1979) Animal tissue techniques. 4th Edn., W. H. Freeman and co., Sanfrancisco, pp: 119-121, 149,210,312-313. ##

10- Hartugan, P. J. Andrews, A. H., Blowey, R. W., Boyd, H. and Eddy, R. G. (1992) Reproduction physiology in cattle, In: Bovine medicine, (Eds), (Blackwell scientific publication) pp.: 420-421.  ##

11- Hyllested, J.L., Veje, K., K. (2002) Ostergaard Histochemical studies of the extracellular matrix of human articular cartilage Osteoarthritis and Cartilage, Volume 10, Issue 5, May  , Pages 333-343.##

12- Lyons, R. A., Djahanbakhch, O., Mahmood, T., Saridogan, E., Sattar, S., Sheaff, M. T., Naftalin, A. A., Chenoy, R.( 2002) Fallopian tube ciliary beat frequency in relation to the stage of menstrual cycle and anatomical site. Hum Reprod. 17(3):584-8.##

13- Makerevich, A. V., & Sirokin, A. V. (1997) The involvement of the GH/IGf-I axis in the regulation of secretory activity by bovine oviduct epithelial cells. Anim. Reprod. Sci. 48(2-4): 197-207. ##

14- Nayak, R. K., & Ellington, E. F.( 1977) Ultrastructural and ultracytochemical cyclic changes in the bovine uterine tube (oviduct) epithelium. Am J Vet Res. 38(2):157-68.  ##

15- Nelcio, A., Tonizza de C., Soares, J. G., Vannuccl, F. S., Angelo, M., Gallupo, A. G., Melo, G. M., Souza, R. J., Nichi M., Gimenes, L. U., Filho, M. F., Martins, C. M., Castriccini, E., Baruselli, P. S. (2012) In vitro oocyte transport through the oviduct of buffalo and crossbred beef cows Braz. J. Vet. Res. Anim. Sci., São Paulo, v. 49, n. 4, p. 277-284##

16- Odor, D. L., Gaddum-Rosse, P., Rumery, R. E.( 1983) Secretory cells of the oviduct of the pig-tailed monkey, Macaca nemestrina, during the menstrual cycle and after estrogen treatment. Am J Anat. 166(2):149-72.  ##

17- Sathananthan, H., Bongso, A., Mok, H., Ratnam, S.( 1990) Ultrastructure of preimplantation human embryos co-cultured with human ampullary cells. Hum Reprod. 5(3):309-18. ##

18- Schultka, R., & Blume, R.( 1978) [Quantitative morphological studies on the variability of the epithelial height of the human Fallopian tube mucosa]. Gegenbaurs Morphol Jahrb. 124(3):420-33.##

19- Shepard, N., & Mitchell, N (1976) Simultaneous localization of proteoglycan by light and electron microscopy using toluidine blue O. A study of epiphyseal cartilage. Journal of Histochemistry and Cytochemistry 61 (11).##

20- Uhrín, V.( 1983) [Changes in the morphology of nuclei in the oviduct epithelium of the cow during the estrous cycle]. Vet Med (Praha). 28(4):211-22. ##

21-Wordinger, R. J., Highman, B., Townsend, J. W., Greenman, D. L.( 1980) The ultrastructure of some histopathological changes seen in oviducts of mice continuously fed diets containing diethylstilbestrol. J Environ Pathol Toxicol. 4(1):169-79. ##

22- Yaniz, J. L., Lopez-Gatius, F., Hunter, R. H.( 2006) Scanning electron microscopic study of the functional anatomy of the porcine oviductal mucosa. Anat Histol Embryol. 35(1):28-34.##