عنوان مقاله [English]
The bovine tuberculosis is one of the most zoonotic diseases which could be infected domestic and wild animals. The causative agent of this infection is Mycobacterium bovis. The correct diagnosis is very critical for effective control of this infection. The usual diagnosis is made by skin test and gamma interferon assay. But other serological techniques based on ELISA could be noted for development of a rapid and correct method. Here, we use MPT-64 recombinant protein as capture antigens for development of an indirect ELISA. The study population consists of thirty two infected and healthy (control) cattle. The infected animals were confirmed by resident veterinary surgeon according to skin test and clinical examination. The levels of specific antibody against MPT-64 were evaluated by indirect ELISA method. According to our results, the levels of specific antibodies against MPT-64 have great consistency with skin test. So that, all of 9 positive samples have high titer for MPT-64 specific antibody and no false negative results have been reported. This study could proposed that development of ELISA method with recombinant MPT-64 antigen could be considered as a rapid, simple and exact method for detection of BTB infected cattle.
1. Bezos J., C. Casal, B. Romero, B. Schroeder, R. Hardegger, A.J. Raeber, L. et al. 2014. Current ante-mortem techniques for diagnosis of bovine tuberculosis. Research in Veterinary Science 97, S44-52.
2. Casal C., A. Díez-Guerrier, J. Álvarez, S. Rodriguez-Campos, A. Mateos, R. Linscott, et al. 2014. Strategic use of serology for the diagnosis of bovine tuberculosis after intradermal skin testing. Veterinary Microbiology 170, 342-51.
3. Collins J.D. 2006. Tuberculosis in cattle: strategic planning for the future. Veterinary Microbiology 112, 369-381.
4. Fu R., C. Wang, C. Shi, M. Lu, Z. Fang, J. Lu, F. Wang et al. 2009. An improved whole-blood gamma interferon assay based on the CFP21-MPT64fusion protein. Clinical and Vaccine Immunology 16, 686-91.
5. Good M and A.Duignan.2011. Perspectives on the History of Bovine TB and the Role of Tuberculin in Bovine TB Eradication. Veterinary Medicine International 2011,410470.
6. Jørstad M.D., M. Marijani, A.M. Dyrhol-Riise, L. Sviland and T. Mustafa. 2018. MPT64 antigen detection test improves routine diagnosis of extra-pulmonary tuberculosis in a low-resource setting: A study from the tertiary care hospital in Zanzibar. PLoS One 13, e0196723.
7. Koo H.C., Y.H. Park, J. Ahn, W.R. Waters, M.V. Palmer, M.J. Hamilton, et al. 2005. Use of rMPB70 protein and ESAT-6 peptide as antigens for comparison of the enzyme-linked immunosorbent, immunochromatographic, and latex bead agglutination assays for serodiagnosis of bovine tuberculosis. Journal of Clinical Microbiology 43, 4498-506.
8. Liu Z., C. Zhu, H. Yang, H. Hu, Y. Feng, L. Qin, et al. 2012. Clinical value of ELISA-MPT64 for the diagnosis of tuberculous pleurisy. Current Microbiology. 65:313-8.
9. Lyashchenko K.P., A. Grandison, K. Keskinen, A. Sikar-Gang, P. Lambotte, J. Esfandiari, G.C. et al. 2017. Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis. Clinical and Vaccine Immunology e00259-17
10. Nerurkar V., S. Kattungal, S. Bhatia. 2016. Utility of MPT64 antigen test for differentiating mycobacteria: Can correlation with liquid culture smear morphology add further value? Clinical and Vaccine Immunology 59:185-7.
11. Parkash O., B.P. Singh and Pai M. 2009. Regions of differences encoded antigens as targets for immunodiagnosis of tuberculosis in humans. Scandinavian Journal of Immunology 70: 345-57.
12. Ramos D.F., P.E. Silva and O.A. Dellagostin. 2015. Diagnosis of bovine tuberculosis: review of main techniques. Brazilian Journal of Biology 75: 830-7.
13. Schiller I., B. Oesch, H.M. Vordermeier, M.V. Palmer, B.N. Harris, K.A. Orloski, B.M. et al. 2010. Bovine tuberculosis: a review of current and emerging diagnostic techniques in view of their relevance for disease control and eradication. Transboundary and Emerging Diseases 57:205-20.
14. Souza I.I., E.S. Melo, C.A. Ramos, T.A. Farias, A.L. Osório, K.S. Jorge, et al. 2012. Screening of recombinant proteins as antigens in indirect ELISA for diagnosis of bovine tuberculosis. Springerplus 1:77.
15. Torgerson P.R. and D.J. Torgerson. 2010. Public health and bovine tuberculosis: what's all the fuss about? Trends in Microbiol 18: 67-72.
16. Une Y and T. Mori. 2007. Tuberculosis as a zoonosis from a veterinary perspective. Comparative immunology, microbiology and infectious diseases 30: 415-25.
17. Vordermeier M., S.V. Gordon and R.G. Hewinson. 2011. Mycobacterium bovis antigens for the differential diagnosis of vaccinated and infected cattle. Veterinary Microbiology 151:8-13.
18. Yan Z.H., L. Yi, P.J. Wei, H.Y. Jia, J. Wang, X.J. Wang, B. et al. 2018. Evaluation of panels of Mycobacterium tuberculosis antigens for serodiagnosis of tuberculosis. The International Journal of Tuberculosis and Lung Disease 22: 959-965.