اثر پودر گیاهان دارویی، تراکم پرورش و آلودگی جیره غذایی با آفلاتوکسین بر فعالیت آنزیم‌های گوارشی ماهی قزل‌آلای رنگین‌کمان

نوع مقاله: مقاله کامل

نویسندگان

1 کارشناس ارشد شیلات، دانشکده منابع طبیعی، دانشگاه ارومیه، ارومیه، ایران

2 دانشیار گروه شیلات و آبزیان، دانشکده منابع طبیعی، دانشگاه ارومیه، ارومیه، ایران

چکیده

در پژوهش حاضر اثر استفاده از پودر گیاهان دارویی (رزماری و آویشن) بر فعالیت آنزیم‌های گوارشی روده‌ی ماهی قزل‌آلای رنگین‌گمان (Oncorhynchus mykiss) تغذیه شده با جیره غذایی آلوده به آفلاتوکسین B1 در تراکم‌های مختلف پرورش مورد ارزیابی قرارگرفت. بدین منظور 600 قطعه ماهی با میانگین وزنی 3±90 گرم، در 6 تیمار آزمایشی شامل دو تراکم پرورش (15 و 45 کیلوگرم بر متر مکعب)، حضور سم آفلاتوکسین در جیره غذایی (0 و 50ppb) به همراه افزودن 4 درصد ترکیب پودر گیاهان دارویی (شامل 2 درصد پودر آویشن شیرازی و2درصد پودر رزماری) تقسیم شدند. هر تیمار در سه تکرار انجام شد و آزمایش به مدت 6 هفته ادامه یافت. پرورش در تراکم بالا (45 کیلوگرم در مترمکعب) و تغذیه با جیره غذایی آلوده به سم آفلاتوکسین موجب افزایش معنی‌دار میزان فعالیت آنزیم‌های گوارشی شامل آمیلاز، لیپاز و آلکالین پروتئاز شد (05/0≥P). همچنین، استفاده از پودر گیاهان آویشن و رزماری در جیره غذایی آلوده به آفلاتوکسین، توانست اثر پاتولوژیک آفلاتوکسین بر افزایش فعالیت آنزیم‌های گوارشی را تعدیل نماید (05/0≥P). با توجه به نتایج حاصل می‌توان بیان داشت که ترکیب برابر پودر گیاهان رزماری و آویشن در سطح مورد مطالعه توانایی کنترل آثار نامطلوب سم آفلاتوکسین بر فعالیت آنزیم‌‌های گوارشی روده ماهیان قزل‌آلای‌رنگین کمان پرورشی در تراکم‌های مختلف را دارد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect medicinal plants powder, stocking density and dietary aflatoxin contamination on digestive enzymes activity of rainbow trout

نویسندگان [English]

  • Zahra Mahmoudikiya 1
  • Ahmad Imani 2
  • kourosh Sarvi Moghanlou 2
1 MSc graduate of Fisheries Science, Department of Fisheries, Faculty of Natural Resources, Urmia University, I.R. of Iran
2 Associate Professor, Department of Fisheries, Faculty of Natural Resources, Urmia University, I.R. of Iran
چکیده [English]

The effect of stocking density and dietary aflatoxin B1 along with rosemary and thyme powders on intestinal digestive enzymes activity of rainbow trout was evaluated. Total of 600 fish with an average weight of 90±3 were randomly allotted among six experimental groups consisted of two stocking density (15 and 45 kg.m-3), aflatoxin contamination (0 and 50 ppb) with/without including 4 % herbal blend powder (2% rosemary and 2% thyme). Each treatment was replicated thrice and the experiment was lasted for 6 weeks. Stocking at high density and receiving aflatoxin B1 contaminated diet resulted in higher digestive enzymes activity including amylase, lipase and alkaline protease (p≤0.05). It seemed that including herbal blend consisting of rosemary and thyme powder could mitigate pathologically elevated digestive enzymes activity due to dietary aflatoxin contamination (p≤0.05). In conclusion, dietary inclusion of rosemary and thyme multi-blend in aflatoxin contaminated diets at the studied level could ameliorate undesirable effects of aflatoxin on intestinal digestive enzymes activity of rainbow trout reared at various densities.

کلیدواژه‌ها [English]

  • Digestive enzymes
  • aflatoxin
  • Shirazi Thyme
  • rosemary
  • Stocking density

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