اثرات نانوذره اکسید مس بر بافت و آنزیم‌های متابولیک کبد و کلیه بچه ماهی کپور معمولی(Cyprinus carpio)

نوع مقاله: مقاله کامل


1 کارشناسی ارشد، دانشگاه گیلان، دانشکده علوم پایه، گروه زیست شناسی

2 دکتری بیولوژی دریا، استادیار دانشگاه گیلان، دانشکده علوم پایه، گروه زیست‌شناسی

3 دکتری بوم شناسی دریا، باشگاه پژوهشگران جوان و نخبگان، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران


امروزه یکی از مشکلات محیط های آبی، ورود فاضلاب‌های صنعتی حاوی نانوذرات است. در این مطالعه اثرات نانوذره‌ی اکسید‌‌مس (CuO NPs) بر برخی آنزیم‌ها و تغییرات بافت‌های کبد و کلیه بچه ماهی کپور معمولی مورد بررسی قرار گرفت. ابتدا سمیت حاد
(LC50 96h)، این نانوذره تعیین شد. سپس بچه ماهیان به مدت 96 ساعت در معرض غلظت‌های 50، 100 و 200 mg/l از CuO NPs به همراه شاهد، هریک با سه تکرار قرار گرفتند. در چهار بازه زمانی بافت‌های کبد و کلیه‌ جدا و بررسی شدند. همچنین سنجش فعالیت آنزیم‌های آلانین آمینوترانسفراز (ALT)، آسپارتات ترانس آمیناز (AST)، آلکالین فسفاتاز (ALP) و لاکتات دهیدروژناز (LDH) در بافت‌ها انجام گرفت. آنالیز فعالیت همه آنزیم ها در بافت کبد در غلظت 200 mg/l پس از 96 ساعت، افزایش معناداری را در مقایسه با شاهد (05/0>p) نشان دادند. برای بافت کلیه، فعالیت آنزیم های ALP و LDH در غلظت 200 mg/l در هر 4 بازه‌ی زمانی و برای آنزیم‌های ALT و AST در غلظت‌های 100 و 200 mg/l پس از 48، 72 و 96 ساعت تفاوت معناداری (05/0>p) نشان دادند. بافت کبد عوارضی چون پرخونی، خونریزی، نکروز سلولی، آتروفی سلولی و دژنرسانس چربی به شدت مشاهده گردید. بافت کلیه، تغییرات بافتی مشابه در کنار کاهش یا تخریب کپسول بومن، انبساط گلومرولی، رسوبات هموسیدرین، نکروز، تخریب و بسته شدن مجاری کلیوی را به شدت نشان داد. نتایج نشان داد مقادیر تحت کشنده نانوذره‌ی اکسیدمس می‌تواند موجب تغییر فعالیت آنزیم‌های شاخص بافت کبد و کلیه ماهی کپور معمولی گردد و آثار هیستوپاتولوژیک چشمگیری برجای بگذارد.



عنوان مقاله [English]

Effects of copper oxide nanoparticles on the tissue and metabolic enzymes of liver and kidney of common carp (Cyprinus carpio)

نویسندگان [English]

  • Samin Khodaei 1
  • Akram Sadat Naeemi 2
  • Fatemeh Nazarhaghighi 3
1 Department of Biology, Faculty of Sciences, Uiversity of Guilan, Rasht, Iran
2 Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran
3 Young Researchers and Elite Club, Rasht Branch, Islamic Azad University, Rasht, Iran
چکیده [English]

Nowadays one of the aquatic environment problems is the entry of industrial wastewater containing nanoparticles. In the present study, the effects of copper oxide nanoparticles (CuO NPs) on some enzymes and histological alterations of liver and kidney tissues in the juvenile carp was assessed. At first, acute toxicity (LC50 96h) of CuO NPs were determined. Then, juvenile carps were exposed to the sublethal concentrations of 50, 100 and 200 mg/l of CuO NPs for 96h. Liver and kidney tissues of carp were separated after four periods. Also, ALT, AST, ALP and LDH enzyme activity assay in tissue extracts was done. The analysis of all enzymes activity in liver at a concentration of 200 mg/l after 96h, showed significant increase compared to the control treatment (P<0/05). In kidney, ALP and LDH enzyme activity for all of the four periods at concentration of 200 mg/l and GOT and GPT enzyme activity after 48, 72 and 96h at concentration of 100 and 200 mg/l showed significant difference. In the acute treatments of liver tissue side effects such as blood congestion, cellular necrosis, and cell atrophy and fatty degeneration was observed. kidney tissue were indicated same histopathological changes and shrinkage or degeneration of Bowman's capsule, glomerular expansion, hemosiderin deposits, tubular necrosis and degeneration or occlusion of the tubular lumen. The results showed that sublethal amounts of CuO NPs could affect the activity of the liver and kidney enzymes of the common carp and showed significant histopathological effects.

کلیدواژه‌ها [English]

  • Copper oxide nanoparticles
  • Kidney
  • liver
  • common carp

1- Abdel-Khalek, A.A., M. A. M. Kadry, S. R. Badran and M. A. S. Marie. 2015. Comparative toxicity of copper oxide bulk and nano particles in Nile Tilapia; Oreochromis niloticus: Biochemical and oxidative stress. Journal of Basic & Applied Zoology 72: 43-57.
2- Abdel-Khalek, A.A., S. R. Badran and M. A. S. Marie. 2016. Toxicity evaluation of copper oxide bulk and nanoparticles in Nile tilapia, Oreochromis niloticus, using hematological, bioaccumulation and histological biomarkers. Fish Physiology and Biochemistry 42:1225-1236.
3- Ahmadi, H. 2016. Effects of copper oxide nanoparticles on some enzyme, hematological factors and gill tissue of common (Cyprinus carpio). MSc thesis. University of Guilan. Rasht, Iran.(In persian)
4- Colvin, V.L. 2003. The potential environment impact of engineered nanomaterials. Nature Biotechnology 21: 1166–1170.
5- Farre, M., K. Gajda-Schrantz, L. Kantiani and D. Barcelo. 2009. Ecotoxicity and analysis of nanomaterials in the aquatic environment. Analytical and Bioanalytical Chemistry 393: 81–95.
6- Gaber, H. S., W. T. Abbas, M. M.N. Authman and S. A. Gaber. 2014. Histological and Biochemical Studies on Some Organs of Two Fish Species in Bardawil Lagoon, North Sinai, Egypt. Global Veterinaria 12: 01-11. DOI: 10.5829/idosi.gv.2014.12.01.76199
7- George, S., H. Gardner, E.K. Seng, H. Chang, C. Wang, C.S.Y. Fang, M., Richards, S. Valiyaveettil and W.K. Chan. 2014. Differential effect of solar light in increasing the toxicity of silver and titanium dioxide nanoparticles to a fish cell line and zebrafish embryos. Environmental Science and Technology 48: 6374–6382.
8- Griffitt, R.J., R. Weil, K.A. Hyndman, N.D. Denslow, K. Powers, D. Taylor and D.S. Barber. 2007. Exposure to copper nanoparticles causes gill injury and acute lethality in zebrafish (Daniorerio). Environmental Science and Technology, 41: 8178–8186.
9- Hoseini, S. M., A. Hedayati, A. Taheri Mirghaed and M. Ghelichpour .2016. Toxic effects of copper sulfate and copper nanoparticles on minerals, enzymes, thyroid hormones and protein fractions of plasma and histopathology in common carp, Cyprinus carpio. Experimental and Toxicologic Pathology 68: 493-503.
10- Howarth, R. S. and J.B. Sprague. 1978. Copper lethality to rainbow trout in waters of various hardness and pH. Water Research 12: 455-462.
11- Javahery, S., H. Nekoubi and A. Haji Moradlu. 2012. Effect of anesthesia with clove oil in fish (review). Fish Physiology Biochemistry 38:1545–1552.
12- Katuli, K.K., B.M. Amiri, A. Massarsky and S. Yelghi. 2014. Impact of a short-term diazinon exposure on the osmoregulation potentiality of Caspian roach (Rutilus rutilus) fingerlings. Chemosphere 108: 396–404.
13- Khabbazi, M., M. Harsij, S.A.A. Hedayati, H. Gholipoor, M. H. Gerami and H. Ghafari Farsani. 2014. Effect of CuO nanoparticles on some hematological indices of rainbow trout (Oncorhynchus mykiss) and their potential toxicity. Journal of Nanomedicine 2: 67-73.
14- Kiaune, L. and N. Singhasemanon. 2011. Pesticidal copper (I) oxide: environmental fate and aquatic toxicity. Reviews of Environmental Contamination and Toxicology 213: 1-26.
15- Klaverkamp, J.F., W.A. MacDonald, D.A. Duncan and R. Wagemann. 1984. Metallothionein and accumulation to heavy metals in fish: a review. Contaminant Effects on Fisheries. pp: 99-113. In: VW.Cairns, PV.Hodson, and JO. Nriagu, (ed.), Wiley, New York.
16- Marina, M. P., B. Camargo Claudia and R. Martinez. 2007. Histopathology of Gills, kidney And Liver of a Neotropical fish caged in an urban stream. Neotropical Ichthyology 5: 227-236.
17- Moore, M.N. 2006. Do nanoparticles present ecotoxicological risks for the health of the aquatic environment?. Environment Internationa 32: 967–976.
18- Murali, M., P. Suganthi, P. Athif, A. Sadiq Bukhari, H.E. Syed Mohamed, H. Basu and R.K. Singhal. 2017. Histological alterations in the hepatic tissues of Al2O3 nanoparticles exposed freshwater fish Oreochromis mossambicus. Journal of Trace Elements in Medicine and Biology 44: 125-131.
19- Murugan, S.S., R. Karuppasamy, K. Poongodi and S. Puvaneswari. 2008. Bioaccumulation Pattern of Zinc in Freshwater Fish Channa punctatus (Bloch.) After Chronic Exposure. Turkish Journal of Fisheries and Aquatic Sciences 8: 55-59.
20- Mustafa, S.A., S.J. Davies and A.N. Jha. 2012. Determination of hypoxia and dietary copper mediated sub-lethal toxicity in carp, Cyprinus carpio, at different levels of biological organization. Chemosphere 87: 413-427.
21- Safaeian, N., M. Shokri and B. Jabaran Amiri. 2004. Environmental Impact Assessment of Development in the Southern Coast of the Caspian Sea (Northern Iran). Polish Journal of environmental studies 13: 45-50.
22- Sattari, M., D. Shahsavani and Sh. Shafiee. 2002. Systematic Ichthyology, Haghshenas publication, Tehran. (In Persian).
23- Yadav, A. K., R. Abbassi, N. Kumar, Sh. Saty, T.R. Sreekrishnane and B.K. Mishra. 2012. The removal of heavy metals in wetland microcosms: Effects of bed depth, plant species, and metal mobility. Chemical Engineering Journal 211-212: 501-507.
24- Zeitoun, M. M., and E. Mehana. 2014. Impact of Water Pollution with Heavy Metals on Fish Health: Overview and Updates. Global Veterinaria 12: 219-231.
25- Zhao, J., Z.Wang, X. Liu, X. Xie, K. Zhang, B. Xing. 2011. Distribution of CuO nanoparticles in juvenile carp (Cyprinus Carpio) and their potential toxicity. Journal of Hazardous Materials 197: 304-310.