تاثیر فیلم کیتوزان-ژلاتین همراه با عصاره پوست انار بر خصوصیات کیفی ماهی شوریده بلانگر (Johnius belangerii) نگهداری شده در دمای 4 درجه سانتی‌گراد

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی کارشناسی ارشد رشته شیلات گرایش فرآوری محصولات شیلاتی، دانشگاه علوم و فنون دریایی خرمشهر، خرمشهر، ایران.

2 استادیار گروه شیلات، دانشکده منابع طبیعی دریا، دانشگاه علوم و فنون دریایی خرمشهر، خرمشهر، ایران.

چکیده

استفاده از فیلم‌‌های خوراکی زیست-کافت از روش‌‌هایی می‌‌باشد که طی سالیان اخیر مورد توجه فراوان قرار گرفته است. قابلیت تشکیل فیلم و خواص حفاظتی ژلاتین و کیتوزان موجب شده که به عنوان فیلم‌‌های ضدمیکروبی و آنتی‌‌اکسیدانی مورد استفاده قرار بگیرند. در مطالعه حاضر، تأثیر فیلم (مخلوط و دولایه) تهیه شده از کیتوزان (1 درصد وزنی/ حجمی) و ژلاتین (3 درصد وزنی/ حجمی) به همراه عصاره پوست انار در نگهداری فیله ماهی شوریده بلانگر در دمای 1 ± 4 درجه سانتی‌گراد یخچال (به مدت 16 روز) مورد بررسی قرار گرفت. 5 تیمار شامل تیمار 1: نمونه شاهد، تیمار2: فیلم مخلوط، تیمار3: فیلم دولایه، تیمار 4: فیلم مخلوط با عصاره پوست انار و تیمار 5: فیلم دو لایه با عصاره پوست انار بسته‌‌بندی شدند. آزمون‌‌های میکروبی (بار باکتریایی کل و سرمادوست) بیانگر تأثیر ضدمیکروبی فیلم‌‌های کیتوزان‌‌-ژلاتین با و بدون عصاره پوست انار بود (05/0>p) اما بین خاصیت ضدمیکروبی انواع مخلوط و دولایه تفاوت معنی‌‌داری وجود نداشت (05/0<p). بار باکتری انتروباکتریاسه فیله پیچیده شده در فیلم کیتوزان-ژلاتین با عصاره پوست انار به طور معنی‌‌داری کمتر از دیگر نمونه‌‌ها بود. آزمون‌‌های شاخص اکسیداسیون چربی (تیوباربیتوریک اسید، اسید چرب آزاد) نیز حاکی از کمتر بودن میزان اکسیداسیون در نمونه‌‌های دارای فیلم کیتوزان-ژلاتین نسبت به نمونه شاهد بود. بنابراین، کیتوزان-ژلاتین به عنوان فیلم خوراکی (مخلوط و دولایه)، ماندگاری فیله Johnius belangerii طی نگهداری در یخچال (1±4 درجه سانتی‌گراد) را افزایش می دهد. بهترین روش پوشش فیله استفاده از فیلم کیتوزان-ژلاتین با عصاره پوست انار بود.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of chitosan-gelatin film combined with pomegranate peel extract on quality properties of Belanger's croaker Johnius belangerii stored at 4 ºC

نویسندگان [English]

  • J. Saki 1
  • Khodanazary A. 2
  • S.M. Hosseini 2
1 Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran.
2 Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran.
چکیده [English]

Interest in biodegradable films has been intensified in recent years. Chitosan and gelatin are biopolymers with preservative effects film forming ability to form antimicrobial and antioxidant films. In the current study, the effect of chitosan (1% w/v) and gelatin (3% w/v) film (composite and bilayer) incorporated with pomegranate peel extract was investigated on the storage of Belanger's croaker fillet in refrigerator (4±1°C) for 16 days. 5 treatment were packed including 1: control sample, 2: composite film, 3: bi-layer film, 4: composite film with pomegranate peel extract, 5: bi-layer film with pomegranate peel extract. Bacterial experiments (total count and pscychrotrophic bacteria) showed the anti bacacterial effect of chitosan-gelatin coating and film with or without pomegranate peel extract (p<0.05) but there was no significant difference between composite and bilayer (p>0.05). The count of Enterobacteriaceae bacteria of chitosan-gelatin film with pomegranate peel extract was lower than other samples. Lipid oxidation value experiments (thiobarbituric acid and free fatty acid values) showed lower oxidation value in film wrapped fillets in comparison with control sample. Thus, chitosan-gelatin as edible film (composite and bilayer) would enhance shelf life of Johnius belangerii fillet during refrigerated (4 ± 1°C) storage. The best of packaging method was the using of chitosan-gelatin film with pomegranate peel extract.

کلیدواژه‌ها [English]

  • Johnius belangerii
  • Bi-layer
  • Composite
  • Shelf life

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