عنوان مقاله [English]
The aim of this study was to evaluate the antimicrobial effects of aqueous extract, ethanol extract and essential oil of Golpar on Salmonella typhimurium isolates from poultry. Plant extracts and essential oils of Golpar were obtained using vacuum from the center (Rotary) and Clevenger. Total number of 12 S. typhimurium strains were isolated from native poultry in the city of Zabol. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of aqueous and ethanol extract and essential oil of Golpar were determined using preparation of different concentrations by dilution in wells method and determination of different concentrations on growth of bacteria. Susceptibility to several antibiotics was evaluated by the Kirby-Bauer disk diffusion standard method. The results of the present study showed that among the essential oils, aqueous extract and ethanol extract of Golpar, the essence have shown highest inhibitory effect on S. typhimurium, while the aqueous extracts have less inhibitory effects on most of the strains of S. typhimurium. The MIC of essential oil was on 5 ppm dilution but for ethanol extract was on 25 ppm and for aqueous extract was on 50 and 100 ppm. The MBC for essential oil, ethanol and aqueous extracts of Golpar were 20, 200 and 200 ppm, respectively. The results showed relevant antibacterial activity of Golpar extracts and essential oils, which can be used for treatment of infections due to the S. typhimurium, instead of common antibiotics.
1. Ginovyan, M., M. Petrosyan, and A. Trchounian. 2017. Antimicrobial activity of some plant materials used in Armenian traditional medicine. BMC Complementary and Alternative Medicine, 17(1): 50.
2. Kong, D. G., Y. Zhao, G. H. Li, B. J. Chen, X. N. Wang, H. L. Zhou, H. X. Lou, D. M. Ren, and T. Shen. 2015. The genus Litsea in traditional Chinese medicine: an ethnomedical, phytochemical and pharmacological review. Journal of Ethnopharmacology, 164: 256-264.
3. Mani, J., S. Kumar, G. J. Dobos, and A. Haferkamp. 2012. Aspects of traditional Indian medicine (Ayurveda) in urology. Urologe A, 51(12): 1663-1673.
4. Dehdari, S. and H. Hajimehdipoor. 2016. Herbal medicines for leucorrhea according to Iranian traditional medicine. Iranian Journal of Medical Sciences, 41(3): S36.
5. DiPasquale, R. 2008. Effective use of herbal medicine in urinary tract infections. Journal of Dietary Supplements, 5(3): 219-228.
6. Na-Bangchang, K. and J. Karbwang. 2014. Traditional herbal medicine for the control of tropical diseases. Tropical Medicine and Health, 42(2): 3-13.
7. Moradi, M. T., M. Rafieian-Kopaei, and A. Karimi. 2016. A review study on the effect of Iranian herbal medicines against in vitro replication of herpes simplex virus. Avicenna Journal of Phytomedicine, 6(5): 506-515.
8. Ebrahimie, M., M. Bahmani, H. Shirzad, M. Rafieian-Kopaei, and K. Saki. 2015. A review study on the effect of Iranian herbal medicines on opioid withdrawal syndrome. Journal of Evidence-Based Complementary & Alternative Medicine, 20(4): 302-309.
9. Amirghofran, Z. 2010. Medicinal plants as immunosuppressive agents in traditional Iranian medicine. Iranian Journal of Immunology, 7(2): 65-73.
10. Hemati, A., M. Azarnia, M. Nabiuni, G. Mirabolghasemi, and S. Irian. 2012. Effect of the hydroalcoholic extract of Heracleum persicum (Golpar) on folliculogenesis in female wistar rats. Cell Journal, 14(1): 47-52.
11. Taghizabet, N., E. Mangoli, F. Anbari, S. A. Masoodi, A. R. Talebi, and M. Mazrooei. 2016. The effect of Heracleum persicum (Golpar) oil and alcoholic extracts on sperm parameters and chromatin quality in mice. International Journal of Reproduction Biomedicine, 14(6): 365-370.
12. Sayyah, M., S. Moaied, and M. Kamalinejad. 2005. Anticonvulsant activity of Heracleum persicum seed. Journal of Ethnopharmacology, 98(1-2): 209-211.
13. Rijal, D. P., T. Alm, S. Jahodova, H. K. Stenoien, and I. G. Alsos. 2015. Reconstructing the invasion history of Heracleum persicum (Apiaceae) into Europe. Molecular Ecology, 24(22): 5522-5543.
14. Shokri, H., A. Sharifzadeh, and I. Ashrafi Tamai. 2012. Anti-Candida zeylanoides activity of some Iranian plants used in traditional medicine. Journal de Mycologie Médicale, 22(3): 211-216.
15. Schikora, A., I. Virlogeux-Payant, E. Bueso, A.V. Garcia, T. Nilau, A. Charrier, S. Pelletier, P. Menanteau, M. Baccarini, P. Velge, and H. Hirt. 2011. Conservation of Salmonella infection mechanisms in plants and animals. PLoS One, 6(9): e24112.
16. Zhang, G., E. Thau, E. W. Brown, and T. S. Hammack. 2013. Comparison of a novel strategy for the detection and isolation of Salmonella in shell eggs with the food and drug administration bacteriological analytical manual method. Poultry Science, 92(12): 3266-3274.
17. Skjolaas, K. A., T. E. Burkey, S. S. Dritz, and J. E. Minton. 2006. Effects of Salmonella enterica Serovars Typhimurium (ST) and Choleraesuis (SC) on chemokine and cytokine expression in swine ileum and jejunal epithelial cells. Veterinary Immunology and Immunopathology, 111(4): 199-209.
18. Fardsanei, F., M. M. Soltan Dallal, M. Douraghi, T. Zahraei Salehi, M. Mahmoodi, H. Memariani, and F. Nikkhahi. 2017. Genetic diversity and virulence genes of Salmonella enterica subspecies enterica serotype Enteritidis isolated from meats and eggs. Microbial Pathogenesis, 107: 451-456.
19. Kang, S. N., G. M. Chu, Y. M. Song, S. K. Jin, I. H. Hwang, and I. S. Kim. 2012. The effects of replacement of antibiotics with by-products of oriental medicinal plants on growth performance and meat qualities in fattening pigs. Animal Science Journal, 83(3): 245-251.
20. Rankin, S. C. 1998. Multiple antibiotic resistance in Salmonella. Veterinary Record, 143(25): 698-699.
21. Berge, A. C., E. L. Dueger, and W. M. Sischo. 2006. Comparison of Salmonella enterica serovar distribution and antibiotic resistance patterns in wastewater at municipal water treatment plants in two California cities. Journal of Applied Microbiology, 101(6): 1309-1316.
22. Santos, U. P., J. F. Campos, H. F. Torquato, E. J. Paredes-Gamero, C. A. Carollo, L. M. Estevinho, K. de Picoli Souza, and E. L. Dos Santos. 2016. Antioxidant, antimicrobial and cytotoxic properties as well as the phenolic content of the extract from Hancornia speciosa gomes. PLoS One, 11(12): e0167531.
23. Miladinovic, D. L., B. S. Ilic, T. M. Mihajilov-Krstev, D. M. Nikolic, O. G. Cvetkovic, M. S. Markovic, and L. C. Miladinovic. 2013. Antibacterial activity of the essential oil of Heracleum sibiricum. Natral Product Communication, 8(9): 1309-1311.
24. Nkuo-Akenji, T., R. Ndip, A. McThomas, and E. C. Fru. 2001. Anti-Salmonella activity of medicinal plants from Cameroon. The Central African Journal of Medicine, 47(6): 155-158.
25. Akinyemi, K. O., U. E. Mendie, S. T. Smith, A. O. Oyefolu, and A. O. Coker. 2005. Screening of some medicinal plants used in south-west Nigerian traditional medicine for anti-Salmonella typhi activity. Journal of Herbal Pharmacotherapy, 5(1): 45-60.
26. Ranjbar, R. and S. Babaie. 2016. Evaluation the antibacterial effects of Echinophora platyloba extracts against some Salmonella species. Electron Physician, 8(2): 1943-1948.
27. Naeini, A., M. Naseri, M. Kamal Nejad, F. Khosh Zaban, T. Rajabian, H. Esmaeil Zadeh Nami, S. Mansouri, and D. Zavieh. 2011. In vitro evaliation of essential oils and extracts of 50 Iranian medicinal plants on standard strains of Candida albicans. Medicinal Plants, 38(2): 163-172 (in Farsi).
28. Sadeghi Nejad, B., M. Rajabi, A. Zarei Mamoudabadi, and M. Zarrin. 2014. In vitro anti-candida activity of the hydroalcoholic extracts of Heracleum persicum fruit against phatogenic candida species. Jundishapur Journal of Microbiol, 7(1): e8703.
29. Zomorodian, K., P. Ghadiri, M. J. Saharkhiz, M. R. Moein, P. Mehriar, F. Bahrani, T. Golzar, K. Pakshir, and M. M. Fani. 2015. Antimicrobial activity of seven essential oils from Iranian aromatic plants against common causes of oral infections. Jundishapur Journal of Microbiology, 8(2): e17766.
30. Valle, D. L., Jr., E. C. Cabrera, J. J. Puzon, and W. L. Rivera. 2016. Antimicrobial activities of methanol, ethanol and supercritical CO2 extracts of philippine Piper betle L. on clinical isolates of gram positive and gram negative bacteria with transferable multiple drug resistance. PLoS One, 11(1): e0146349.