عنوان مقاله [English]
Newcastle disease is one of the most important diseases and challenges in poultry farming in Iran. This cross-sectional study was conducted from March 2014 to June 2017 in broiler farms in Mazandaran province to evaluate the serum titers against Newcastle disease vaccine under field conditions. At least 16 blood samples were taken from each farm; a hemagglutination inhibition (HI) test was performed on serum samples. The mean titers were compared within different months using ANOVA and Tukey's test. 224 broiler farms and 3573 birds were sampled. In terms of age, 22 flocks were less than 20 days old, 8 flocks were between 20 and 24 days, 91 flocks 25 to 34 days, and 103 flocks were 35 days and older. The mean titer of the sampled flocks was 5.38 ± 2.14. The mean titer of 31 farms (13.8%) was less than 3, 103 farms (46.98%) was between 3 and 6, and 90 farms (40.17%) was 6 and higher. In 8 farms, velogenic Newcastle viruse (57.7%) was identified using RT-PCR. The average of titers of the flocks aging 34-35 days was 4.94 and there was no significant difference in mean titers of flocks older than 35 days (5.71) (P> 0.05). The mean titer of all sampled flocks in the province (5.38) was significantly higher than 3 as the protective titer (P <0.001). In conclusion, it seems that the vaccination programs of the flocks sampled during this study, had a good coverage and prevented the occurrence of an epidemic in the region.
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