عنوان مقاله [English]
The cause of tyzzer's disease is Clostridium piliforme bacteria. Since the international standards on determination of contamination in laboratory animals in Iran It's not done.This study intends review the incidence of Clostridium piliforme infection in laboratory NIH mice colonies in a laboratory animals breeding center. 82 mice from a colony of breeding laboratory animals were randomly selected and were examined by indirect ELISA method for specific antibodies against Clostridium piliforme in serum. Also after necropsy the gastrointestinal were studied examined for symptoms of the disease. Of these, two positive cases were identified in ELISA and any symptoms of disease was not observed in necropsy. According to the FELASA instructions, it can be concluded with 99.9% confidence, the contamination with Clostridium piliforme in breeding facilities, much less than 10% (2.43%) in the NIH mice colony is obtained and is not need to remove the colony. However, should be considered carefully and be more sensitive to this bacteria in periodic testing.
1- Fallahi, R. and Mansouri, M.A. (2015) Biology, Breeding, Diseases and Principles of Working to Laboratory Animals, First Edition, Razi Vaccine and Serum Research Institute Publication, pp: 68-109.
2- Allen, A.M., Ganaway, J.R,, Moore, T.D. and Kinard, R.F. (1965) Tyzzer’s disease syndrome in laboratory rabbits, The American Journal of Pathology, 46:859–882.
3-Boot, R. (2001) Development and validation of ELISAs for monitoring bacterial and parasitic infections in laboratory rodents and rabbits, Scandinavian Journal of Laboratory Animal Science, 1(28), 1-8.
4-Boot, R., van Herck, H.and van der Logt, J.(1996) Mutual viral and bacterial infections after housing rats of various breeders within an experimental unit, Laboratory Animal, 30(1), 42-45.
5-Boivin, G.P., R.R. Hook, J. R. and Riley٫ L.K. (1994) Development of a monoclonal antibody-based competitive inhibition ELISA for detectionof Bacillus piliformis isolate-specific antibodies in laboratory animals, Laboratory Animal Science, 44,153-158.
6- de Bruin W.C., van de Ven, E.M.and Hooijmans, C.R.(2016) Efficacy of soiledbedding transfer for transmission of mouse and rat infections tosentinels: A Systematic Review, PLoS One, 12;11(8): e0158410. doi: 10.1371/journal.pone.0158410.
7-Dammann, P., Hilken, G., Hueber, B., Kohl, W., Bappert, M.T.and Mahler, M. (2011). Infectious microorganisms in mice (Mus musculus) purchased from commercial pet shops in Germany, Laboratory Animal, 45(4):271-5. doi: 10.1258/la.2011.010183. Epub 2011 Apr 20٫www.ncbi.nlm.nih.gov.
8- Feldman, S.H., Kiavand, A., Seidelin, M.and Reiske, H.R. (2006) Ribosomal RNA sequences of Clostridium piliforme isolated from rodent and rabbit: re-examining the phylogeny of the Tyzzer's disease agent and development of a diagnostic polymerase chain reaction assay, The Journal of the American Association for Laboratory Animal Science. 45(5):65-73٫www.ncbi.nlm.nih.gov.
9- FELASA Working group on health monitoring of rodent and rabbit colonies (2014) Recommendations on health monitoring of rodent and rabbit colonies in breeding and experimental units, Laboratory Animals aine, 36: 20-42.
10-Goto, K. and Itoh, T. (1996) Detection of Clostridium piliforme by enzymatic assay of amplified cDNA segment in microtitration plates٫ Lab Anim Sci. 46(5):493-6٫www.ncbi.nlm.nih.gov.
11- Hubrecht, R. and Kirkwood, J. (2010) The UFAW hand book on the care and management of laboratory and oher research animals. 8th Edition, The laboratory mouse, pp: 304-309.
12-Hansen, A.K., Andersen, H.V.and Svendsen, O. (1994) Studies on the diagnosis of Tyzzer's disease in laboratory rat colonies with antibodies against Bacillus piliformis (Clostridium piliforme), Laboratory Animal Science. Oct; 44(5):424-9٫www.ncbi.nlm.nih.gov٫1395/8
13- Hansen, A.K. (2000) Handbook of laboratory animal bacteriology, Chapter nine, 9.2.1.Clostridium piliforme CRC Press, Medical / Nursing.
14- Kahn, C.M. (2010) The Merck Veterinary Manual, Digestive system, Tyzzer disease, 10th ed. Hoboken, New Jersey: John Wiley and Sons.
15- Livingston, R.S, Franklin, C.L., Besch-Williford, C.L., Hook, R.R.and Riley, L.K.(1998) A novel presentation of Clostridium piliforme infection (Tyzzer's disease) in nude mice. Laboratory Animal Science. 48(4):334-339.
16-Motzel, S.L., Meyer, J.K.and Riley, L.K. (1991) Detection of serum antibodies to Bacillus piliformis in mice and rats using an enzyme-linked immunosorbent assay, Laboratory Animal Science, 41(1):26-30.
17-Spencer, T.H., Ganaway, J.R. and Waggie, K.S. (1990) Cultivation of Bacillus piliformis (Tyzzer) in mouse fibroblasts (3T3 cells) Veterinary Microbiology, 22:291–297.