عنوان مقاله [English]
The objectives of present study was to evaluate the association among blood metabolites, tumor necrosis factor (TNF)-alpha and metabolic diseases in Holstein cows during transition period. Pregnant multiparous Holstein cows (N = 50), with body condition scores (BCS) 3.5±0.75 and third parity were selected. Blood samples were collected weekly from 2 week prior to calving to 2 weeks after calving to determine plasma concentrations of non-esterifies fatty acids (NEFA), beta-Hydroxyl butyrate acid (BHBA) and activity of aspartate aminotransferase (AST) and alanine aminotrans-ferase (ALT) enzymes. According to above parameters cows divided into healthy (N =10) and diseases cow (n= 10) and plasma concentrations of glucose, insulin and TNF-α compared weekly from 2 week prior to calving to 2 weeks after calving.Results showed cows with metabolic disorders had higher plasma concentrations of NEFA, BHBA, AST and TNF-alpha compared to healthy cows (P < 0.05) but conversely plasma concentrations of glucose and insulin were lower in cows with metabolic disorders compared to healthy cows (P < 0.05). Plasma glucose concentration affected by time and increased from -14 to calving in both treatments and after that decreased until d +14. Glucose concentrations at d +7 and +14 were greater in healthy cows compared to cows with metabolic disorders. Plasma TNF-α concentration were greater in cows with metabolic disorders compared to healthy cows during whole esxperiment. In conclusion due to greater TNF-α concentration in cows with metabolic disorders before calving, using this item could be useful to predict cows suspicious to metabolic disorders after calving therefore we can use suitable strategies to prevent or decreased incidence of metabolic disorders.
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