بررسی تاثیر سین‌بیوتیک (Biomin Imbo) بر وضعیت آنتی اکسیدان و پروفایل لیپیدی بچه ماهیان کفال معمولی (Mugil cephalus)

نوع مقاله: مقاله کامل


دانشگاه دریانوردی و علوم دریایی چابهار، دانشکده علوم دریایی، گروه شیلات


تحقیق حاضر به‌منظوز بررسی تاثیرسین‌بیوتیک (Biomin Imbo) بر وضعیت آنتی‌اکسیدان و پروفایل لیپیدی بچه‌ماهیان کفال خاکستری (Mugil cephalus) به مدت 60 روز صورت گرفت. 120 قطعه بچه‌ماهی کفال خاکستری با میانگین وزنی 43/0±92/3 گرم در یک طرح کاملا تصادفی با 4 تیمار و 3 تکرار (با تعداد 10 قطعه در هر تکرار) که شامل تیمار 1 (گروه شاهد)، تیمار 2 (5/0 گرم سین بیوتیک در هر کیلوگرم غذا)، تیمار 3 (1 گرم سین بیوتیک در هر کیلوگرم غذا) و تیمار 4 (5/1 گرم سین بیوتیک در هر کیلوگرم غذا) بود مورد آزمایش قرار گرفتند. در پایان آزمایش، کمترین میزان کلسترول و تری‌گلیسرید کبد در تیمار 3 مشاهده شد و بین تیمار‌ها تفاوت معنی‌داری وجود داشت (05/0>P). کمترین میزان لیپوپروتئین با چگالی پایین و بیشترین میزان لیپوپروتئین با چگالی بالا در تیمار 3 مشاهده شد و بین تیمارها تفاوت معنی‌داری وجود داشت (05/0>P). بیشترین میزان فعالیت گلوتاتیون احیاء، ظرفیت آنتی‌اکسیدانی کل در تیمار 3 مشاهده شد. اما بین تیمار 3 و 4 اختلاف معنی‌داری مشاهده نشد (05/0<P). همچنین از نظر فعالیت کاتلاز بین تیمار‌های حاوی سین‌بیوتیک و شاهد اختلاف معنی‌داری مشاهده نشد (05/0<P). در کل، نتایج نشان داد که استفاده از 1 گرم سین‌بیوتیک در هر کیلوگرم جیره غذایی منجر به بهبود کلسترول، تری‌گلیسرید، لیپوپروتئین با چگالی پایین و فعالیت آنتی‌اکسیدانی سرم کبد ماهی کفال خاکستری می‌شود.


عنوان مقاله [English]

Effect of Synbiotic Biomin Imbo on anti-oxidant status and lipid profiles of Mugil cephalus

نویسندگان [English]

  • P. Akbary
  • S. Bita
  • M. Sarhadipour
Chabahar Maritime University, Marine Science, Fisheries group, Iran.
چکیده [English]

This experiment was conducted to evaluate the effect of Synbiotic Biomin Imbo on anti-oxidant status and lipid profiles of Mugil cephalus for 60 days. The experiment was conducted in a completely randomized design with 120 gray mullet (with an average weight of 3.92 ± 0.43 g) in 4 treatments and 3 replicates (n=10 in each replicate) and included: treatment 1 (control), treatment 2 (0.5 g Synbiotic per kg diet), treatment 3 (1 g Synbiotic per kg diet) and treatment 4 (1.5 g synbiotic per kg diet). At the end of experiment, the highest and lowest cholesterol and triglyceride of liver fish were recorded in treatment 1 and 4 respectively and there was significant difference between treatments (P< 0.05). The lowest low density of lipoprotein (LDL) and the highest high density of lipoprotein (HDL) were recorded in treatment 3 (P< 0.05). The highest GSH and TAC were observed in treatment 3 but there was no significant difference between treatment 3 and 4. The anti- oxidant status findings suggest that using 1 g Synbiotic per kg diet is effective in improving anti- oxidant status and lipid profiles in gray mullet.

کلیدواژه‌ها [English]

  • Mugil cephalus
  • Cholesterol
  • Triglyceride
  • Anti- oxidant status
  • Low density lipoprotein

1. Akerboom, T.P. and H. Sies. 1981. Assay of glutathione, glutathione disulfide, and glutathionemixed disulfides in biological samples. Methods Enzymology 77: 373–382.
2. Ai, Q., H. Xu, K. Mai, W. Xu, J. Wang and W. Zhang. 2011. Effects of dietary supplementation of Bacillus subtilis and fructooligosaccharide on growth performance, survival, non-specific immune response and disease resistance of juvenile large yellow croaker, Larimichthys crocea. Aquaculture 317: 155-161.
3. Burtis, C.A., E.R. Ashwood and D.E. Brund. 1994. Tietz Textbook of Clinical Chemistry (5thed.).W.B. Sunders Company. Philadelphia, USA.
4. Castelli, W.P. 1977. HDL Cholesterol and other lipids in coronary heart disease. Circulation 55:767-772.
5. Chitsaz, H., R. Akrami and M. Arab Arkadeh. 2016. Effect of dietary synbiotics on growth, immune response and body composition of Caspian roach (Rutilus rutilus). Iranian Journal Fisheries Sciences 15: 170-182.
6. Denev, S., Y. Staykov, R. Moutafchieva and G. Beev. 2009. Microbial ecology of the gastrointestinal tract of fish and the potential application of probiotics and prebiotics in finfish aquaculture. International of Aquatic Research 1:1–29.
7. Dimitroglou, A., D.L. Merrifield, P. Spring, J. Sweetman, R. Moate and S.J. Davies. 2010. Effects of mannan oligosaccharide (MOS) supplementation on growth performance, feed utilization, intestinal histology and gut microbiota of gilthead sea bream (Sparus aurata). Aquaculture 300:182–188.
8. Draper, H.H., E.J. Squires, H. Mahmoodi, J. Wu, S. Agarwal and M. Hadley. 1993. A comparative evaluation of thiobarbituric acid methods for the determination of malondialdehyde in biological materials, Free Radical Biology and Medicine 15: 353-363.
9. Gibson, G.R. and M.B. Roberfroid. 1995. Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics.Journal of Nutrition 125: 1401–1412.
10. Haghighi, D.H., M. Fallahi and Y. Abdollahtabar. 2010. The effect of different levels of Biomin Imbo synbiotic on growth and, survival of Rutilus frisii kutum fry. Journal of Fisheries Islamic Azad University, Azadshahr Branch. 4: 1-15.
11. Herna´ndez Serrano, P. 2005. Responsible Use of Antibiotics in Aquaculture, FAO Fisheries Technical Paper. FAO, Rome.
12. Kavitha, K., A. G. Reddy, K. K. Reddy, C. S. Kumar, G. Boobalan and K. Jayakanth. 2016. Hypoglycemic, hypolipidemic and antioxidant effects of pioglitazone, insulin and synbiotic in diabetic rats. Veterinary World 9(2): 118-121.
13. Kim, D. and B. Austin. 2006. Innate immune responses in rainbow trout (Oncorhynchus mykiss, Walbaum) induced by probiotics. Fish and Shellfish Immunology 21: 513–524.
14. Lara-flores, M., M. A. Olvera-Novoa, B. E. Guzmán Méndez and W. López- Madrid. 2003. Use of the bacteria Streptococcus faecium and Lactobacillus acidophilus, and the yeast Saccharomyces cerevisiae as growth promoters in Nile tilapia (Oreochromis niloticus). Aquaculture 216:193-201.
15. Liong, M. T. and N. P. Shah. 2005. Acid and bile tolerance and cholesterol removal ability of lactobacilli strains. Journal of dairy science 88(1): 55-66.
16. Liong, M. T., F. R. Dunshea and N. P. Shah. 2007. Effects of a synbiotic containing Lactobacillus acidophilus ATCC 4962 on plasma lipid profiles and morphology of erythrocytes in hypercholesterolaemic pigs on high-and low-fat diets. British Journal of Nutrition 98(4): 736-744.
17. Marklund, S. and G. Marklund. 1974. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry 47:469-474.
18. Martı´nez-A lvarez R.M., A.E. Morales, and A. Sanz. 2005. Antioxidant defenses in fish: biotic and abiotic factors. Review Fish Biology and Fisheries15:75–88.
19. Mehrabi, Z., F. Firouzbakhsh and A. Jafarpour. 2012. Effects of dietary supplementation of synbiotic on growth performance, serum biochemical parameters and carcass composition in rainbow trout (Oncorhynchus mykiss) fingerlings. Journal of Animal Physiology and Animal Nutrition 96: 474-481.
20. Ooi, L.G. and M.T. Liong. 2010. Cholesterol-lowering effects of probiotics and prebiotics: a review of in vivo and in vitro findings. International Journal of Molecular Sciences 11: 2499–2522.
21. Pereira, D. I. and G. R. Gibson. .2002. Cholesterol assimilation by lactic acid bacteria and bifidobacteria isolated from the human gut. Applied and environmental microbiology 68(9): 4689-4693.
22. Porfaraj, V., M. Karami, S.A. Nezami, G.R. Rafiee, H. Khara and A. Hamidoghli. 2013. Study of some biological features of Mullets in Iranian coasts of the Caspian sea. Journal of Utilization and Cultivation of Aquatics 2: 97-110.
23. Reyes-Becerril, M., I. Salinas, A. Cuesta, J. Meseguer, D. Tovar-Ramirez, F. Ascencio-Valle and M.A. Esteban. 2008. Oral delivery of live yeast Debaryomyces hansenii modulates the main innate immune parameters and the expression of immune-relevant genes in the gilthead seabream (Sparus aurata L.). Fish and Shellfish Immunology 25: 731-739.
24. Ringø, E., R.E. Olsen, T.Ø. Gifstad, R.A. Dalmo, H. Amlund, G.I. Hemre and A.M. Bakke. 2010. Prebiotics in aquaculture: a review. Aquaculture Nutrition 16:117–136.
25. Safari, O., M. Mehraban Sang Atash and M. Paolucci. 2015. Effect of dietary L- carnitine level on growth performance, immune responses and stress resistance of juvenile narrow clawed crayfish, Astacus Leptodactylus leptodactylus Eschcholtz, 1823. Aquaculture 439: 20-28.
26. Torrecillas, S., A. Makoi, M.J. Cabaliero, D. Montero, L. Robaina and L.Tort. 2007. Immunostimulations and improved infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides. Fish and Shellfish Immunology 23:969–981.
27. Trinder, P. 1969. Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Annuals of Clinical Biochemistry 6: 24-27.
28. Vitali, B., M. Ndagijimana, S. Maccaferri, E. Biagi, M.E. Guerzoni and P. Brigidi. 2012. An in vitro evaluation of the effect of probiotics and prebiotics on the metabolic profile of human microbiota. Anaerobe 18: 386-391.
29. Wang, Y., J. Li and J. Lin. 2008. Probiotics in aquaculture: challenges and outlook. Aquaculture 281:1–4.
30. Weifen, L., Z. Xiaoping, S. Wenhui, D. Bin, L. Quan, F. Luoqin, Z. Jiajia, W. Yue and Y. Dongyou. 2012. Effects of Bacillus preparations on immunity and antioxidant activities in grass carp (Ctenopharyngodon idellus). Fish Physiology and Biochemistry 38: 1585-1592.
31. Ye, J. D., K. Wang, F.D. Li and Y.Z. Sun. 2011. Single or combined effects of fructo‐and mannan oligosaccharide supplements and Bacillus clausii on the growth, feed utilization, body composition, digestive enzyme activity, innate immune response and lipid metabolism of the Japanese flounder Paralichthys olivaceus. Aquaculture nutrition 17(4): 902-911
32. Zhang, C.N., X.F. Li, W.N. Xu, G.Z. Jiang, K.L. Lu, L.N. Wang and W.B. Liu. 2013. Combined effects of dietary fructooligosaccharide and Bacillus licheniformis on innate immunity, antioxidant capability and disease resistance of triangular bream (Megalobrama terminalis). Fish and Shellfish Immunology 35: 1380-1386.