شناسایی آلودگی جوجه‌های گوشتی به سویه‌های بسیار حاد بیماری بورس قبل از واکسیناسیون

نوع مقاله : مقاله کامل

نویسنده

بخش تحقیقات بیماری‌های ویروسی طیور، موسسه تحقیقات واکسن و سرم سازی رازی. سازمان تحقیقات، آموزش و ترویج کشاورزی. تهران. ایران

چکیده

در این مطالعه آلودگی گله‌های گوشتی به ویروس بیماری بورس عفونی قبل از واکسیناسیون مورد بررسی قرار گفت. از 25 گله مرغ گوشتی در استان تهران 10 پرنده 12-10 روزه قبل از واکسیناسیون انتخاب و پس از وزن‌کشی نمونه‌های بورس گرفته شد. نمونه‌های بورس اخذ شده؛ جهت ردیابی ویروس با آزمایشات مولکولی RT-PCR مورد استفاده قرار گرفتند. نتایج آزمایشات RT-PCR که با استفاده از پرایمرهای اختصاصی ؛ قطعه‌ی 643 جفت بازی از منطقه‌ی متغیر VP2 تکثیر شد؛ نشان داد که دو گله 4 و9 از نظر وجود ژنوم ویروس بیماری بورس عفونی مثبت بودند. برای شناخت بیشتر ویروس ردیابی شده چگونگی هضم آنزیمی محصولات PCR توسط آنزیمهای اندونوکلئاز SacI و SspI مورد مطالعه قرار گرفت. نتایج هضم آنزیمی نشان داد که محصولات PCR به دست آمده از نمونه‌های 4 و 9 توسط آنزیم SacI شکسته نشد. با این همه آنزیم SspI توانست تنها محصولات PCR یکی از جدایه‌ها را برش دهد (گله 9) در حالی‌که نمونه 4 توسط این آنزیم هضم نشد. میانگین شاخص بورس این دو گله کمتر از گله‌های آلوده نشده بود منتهی تنها شاخص بورس گله‌ی 4 به طور معنی‌دار از گله‌های غیر آلوده کمتر بود (001/0 > p). با توجه به نتایج این مطالعه می‌توان اظهار داشت که امکان آلوده شدن گله‌های مرغ گوشتی به ویروس‌های بسیار حاد این بیماری قبل از واکسیناسیون وجود دارد.

کلیدواژه‌ها


عنوان مقاله [English]

Survey to Detect Very Virulent Infectious Bursal Disease in Broiler Flocks Before Vaccination

نویسنده [English]

  • A. H. Shoushtari
Department of Poultry Viral Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Tehran-Iran.
چکیده [English]

This study was conducted to detect the infection with infectious bursal disease virus (IBDV )in broiler flocks before vaccination in Tehran provience . Ten birds of 10 to 12 days olds from each 25 boiler flocks were selected .The mean bursal indexes of all flocks were determined. To detect IBDV genome in bursal samples a RT – PCR test targeting a 643 base pairs segment in VP2 gene were conducted. Two flocks( samples 1 and 2) were posiive indicating IBDV infection. For Further indentification of IBDV , the RT-PCR products were incubated to be digested by endonuclease enzyms SacI and SspI respectively. The RT-PCR products of positive samples ( samples 4 and 9) remaind undigested when were incubated with SacI, an indication for non vaccinal viruses. However only the RT-PCR products of sample 9 could be digested by SspI as the RT-PCR of samples 4 remaind undigested. Although the mean bursal indexes of these two infected flocks were less than those of noninfected ones, however only mean bursal indexes of the flock 4 were significantly different from those of non-infected flocks. In conclusion, this study showed that infection of broilers with very virulent pathotype of IBDV is probable before vaccination.

کلیدواژه‌ها [English]

  • Infectious bursal disease virus
  • broilers
  • FLP- Nested PCR- RT-PCR
  • Bursal Index
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