ارزیابی عملکرد تنکرد شناسی غده تیروئید در ماهی بیاح (Liza abu) خور موسی

نوع مقاله: مقاله کامل

نویسندگان

1 کارشناس ارشد، گروه زیست دریا، دانشکده علوم دریایی و اقیانوسی، دانشگاه علوم و فنون دریایی خرمشهر، خرمشهر

2 استادیار، گروه زیست دریا، دانشکده علوم دریایی و اقیانوسی، دانشگاه علوم و فنون دریایی خرمشهر، خرمشهر

3 دانشیار، گروه زیست دریا، دانشکده علوم دریایی و اقیانوسی، دانشگاه علوم و فنون دریایی خرمشهر، خرمشهر

چکیده

هورمون‌های محور هیپوفیز-تیروئید نقش مهمی در حفظ شرایط طبیعی بدن مهره‌داران دارند. از این‌رو اختلال در عملکرد این محور می‌تواند بر سایر بخش‌های بدن تاثیر گذارد. در تحقیق حاضر، 50 قطعه ماهی بیاح نر و ماده (میانگین وزنی2/8 ± 51/45 گرم و میانگین طولی 88/0±25/16 سانتی-متر) از پنج ایستگاه در خور موسی شامل: 1) پتروشیمی 2) غنام 3) زنگی 4) دورق 5) پاتیل و همچنین 10 قطعه ماهی بیاح نیز از خور سجافی واقع در خور موسی به عنوان ایستگاه شاهد جمع آوری شد. پس از خون‌گیری و جداکردن پلاسمای خون ماهیان، سطح هورمون‌های تری یدوتیرونین (T3) و تترایدوتیرونین (T4) به روش الایزا در پلاسمای خون این ماهیان مورد سنجش قرار گرفت. همچنین، نمونه‌هایی از بافت تیروئید ماهیان اخذ شده، در فرمالین 15 درصد تثبیت گردید و بر اساس روش مرسوم بافت شناسی مورد مطالعه قرار گرفت. در پلاسمای ماهیان جمع آوری شده از ایستگاه پاتیل به ترتیب کمترین و بیشترین مقدار هورمون های T3 و T4 مشاهده شد. همچنین تغییرات آسیب‌شناسی بافتی (هیستوپاتولوژیک) مانند خون ریزی در ماهیان صید شده از خور پتروشیمی مشاهده گردید. نتایج این تحقیق نشان دهنده اثرات منفی آلاینده‌های محیطی بر تعادل هورمون‌های تیروئیدی بود.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of histophysiological function of thyroid in Liza abu from Musa creek

نویسندگان [English]

  • N. Koosha 1
  • N. Salamat 2
  • M. T. Ronagh 2
  • A. A. Movahedinia 3
1 Master, Department of Marine Biology, Faculty of Marine and Oceanic Science, Khorramshahr University of Marine Science and Technology, khorramshahr
2 Assistant Professor, Department of Marine Biology, Faculty of Marine and Oceanic Science, Khorramshahr University of Marine Science and Technology, khorramshahr.
3 Associate Professor, Department of Marine Biology, Faculty of Marine and Oceanic Science, Khorramshahr University of Marine Science and Technology, Khorramshahr.
چکیده [English]

The hormones of pituitary-thyroid axis play an important role in the maintaining of the normal physiological status of the body in vertebrates. Therefore, disturbance in the function of this axis can affect other parts of the body. In the present study, 50 male and female Liza abu (with 45.51 ± 8.2 g body weight and 16.25 ± 0.88 cm body length) were collected from five stations in Musa creek including: 1) Petrochemical 2) Ghanam 3) Zangi 4) Doragh and 5) Patil stations. 10 L. abu were also caught at the Sajafi station (located in Musa creek) as a control site. Bleeding was carried out and the blood plasma was separated. Then, the plasma levels of thyroid hormones (triiodothyronine (T3) and tetraiodothyronine (T4)) were measured using ELISA method. Tissue samples were also taken from thyroid and were fixed in 15% formalin and prepared according to the routine histological process. The lowest and highest amounts of T3 and T4 hormones respectively, were measured in the fish plasma from Patil station. The most histopathological changes (such as hemorrhage) were observed in the fish from Petrochemical station. The results of this study indicated the adverse effects of environmental pollution on the balance of thyroid hormones.

کلیدواژه‌ها [English]

  • Pituitary-thyroid axis
  • Liza abu
  • Triiodothyronine
  • Tetraidothyronin
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