ارزیابی عملکرد تنکرد شناسی غده تیروئید در ماهی بیاح (Liza abu) خور موسی

نوع مقاله : مقاله کامل

نویسندگان

1 کارشناس ارشد، گروه زیست دریا، دانشکده علوم دریایی و اقیانوسی، دانشگاه علوم و فنون دریایی خرمشهر، خرمشهر

2 استادیار، گروه زیست دریا، دانشکده علوم دریایی و اقیانوسی، دانشگاه علوم و فنون دریایی خرمشهر، خرمشهر

3 دانشیار، گروه زیست دریا، دانشکده علوم دریایی و اقیانوسی، دانشگاه علوم و فنون دریایی خرمشهر، خرمشهر

چکیده

هورمون‌های محور هیپوفیز-تیروئید نقش مهمی در حفظ شرایط طبیعی بدن مهره‌داران دارند. از این‌رو اختلال در عملکرد این محور می‌تواند بر سایر بخش‌های بدن تاثیر گذارد. در تحقیق حاضر، 50 قطعه ماهی بیاح نر و ماده (میانگین وزنی2/8 ± 51/45 گرم و میانگین طولی 88/0±25/16 سانتی-متر) از پنج ایستگاه در خور موسی شامل: 1) پتروشیمی 2) غنام 3) زنگی 4) دورق 5) پاتیل و همچنین 10 قطعه ماهی بیاح نیز از خور سجافی واقع در خور موسی به عنوان ایستگاه شاهد جمع آوری شد. پس از خون‌گیری و جداکردن پلاسمای خون ماهیان، سطح هورمون‌های تری یدوتیرونین (T3) و تترایدوتیرونین (T4) به روش الایزا در پلاسمای خون این ماهیان مورد سنجش قرار گرفت. همچنین، نمونه‌هایی از بافت تیروئید ماهیان اخذ شده، در فرمالین 15 درصد تثبیت گردید و بر اساس روش مرسوم بافت شناسی مورد مطالعه قرار گرفت. در پلاسمای ماهیان جمع آوری شده از ایستگاه پاتیل به ترتیب کمترین و بیشترین مقدار هورمون های T3 و T4 مشاهده شد. همچنین تغییرات آسیب‌شناسی بافتی (هیستوپاتولوژیک) مانند خون ریزی در ماهیان صید شده از خور پتروشیمی مشاهده گردید. نتایج این تحقیق نشان دهنده اثرات منفی آلاینده‌های محیطی بر تعادل هورمون‌های تیروئیدی بود.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of histophysiological function of thyroid in Liza abu from Musa creek

نویسندگان [English]

  • N. Koosha 1
  • N. Salamat 2
  • M. T. Ronagh 2
  • A. A. Movahedinia 3
1 Master, Department of Marine Biology, Faculty of Marine and Oceanic Science, Khorramshahr University of Marine Science and Technology, khorramshahr
2 Assistant Professor, Department of Marine Biology, Faculty of Marine and Oceanic Science, Khorramshahr University of Marine Science and Technology, khorramshahr.
3 Associate Professor, Department of Marine Biology, Faculty of Marine and Oceanic Science, Khorramshahr University of Marine Science and Technology, Khorramshahr.
چکیده [English]

The hormones of pituitary-thyroid axis play an important role in the maintaining of the normal physiological status of the body in vertebrates. Therefore, disturbance in the function of this axis can affect other parts of the body. In the present study, 50 male and female Liza abu (with 45.51 ± 8.2 g body weight and 16.25 ± 0.88 cm body length) were collected from five stations in Musa creek including: 1) Petrochemical 2) Ghanam 3) Zangi 4) Doragh and 5) Patil stations. 10 L. abu were also caught at the Sajafi station (located in Musa creek) as a control site. Bleeding was carried out and the blood plasma was separated. Then, the plasma levels of thyroid hormones (triiodothyronine (T3) and tetraiodothyronine (T4)) were measured using ELISA method. Tissue samples were also taken from thyroid and were fixed in 15% formalin and prepared according to the routine histological process. The lowest and highest amounts of T3 and T4 hormones respectively, were measured in the fish plasma from Patil station. The most histopathological changes (such as hemorrhage) were observed in the fish from Petrochemical station. The results of this study indicated the adverse effects of environmental pollution on the balance of thyroid hormones.

کلیدواژه‌ها [English]

  • Pituitary-thyroid axis
  • Liza abu
  • Triiodothyronine
  • Tetraidothyronin
1. Abdollahpour, F.(1389).Study comparative of the accumulation of eavy metals (Cd, Cu, Co, Ni, Pb) in the sediment and dominant species of fish dishes Moses, master's thesis. Khorramshahr University of Marine Science and Technology. 86 p.
2. Abrahams, M.V. and Pratt., T.C. (2000). Hormonal manipulations of growth rate and its influence on predator avoidance Foraging tradeoffs. Can. J. Zool. 78(1): 121–127p.
3. Azimi, A., Dadolahi, S.A., Safahieh, A., Zolgharnein, H., Savari, A. and Faghiri, I. (2012). The study of heavy metals (Hg, Cd, Pb and Cu) Levels in Sediments of North- West of Persian gulf- Imam Khomeini Port. 3:10-17p.
4. Babadi, P. (2011). Biological accumulation of mercury insometissues of yellow fin eabream (Acanthopagrus latus) compared tothe natural environment of the laboratory, a Master's thesis. Khorramshahr University of Marine Science and Technology. 75p.
5. Bancroft, J. D., Gamble, M. (2008). Theory and practice of histological techniques. Elsevier Health Sciences.
6. Blanton, M.L. and Specker, J.L. (2007). The Hypothalamic-Pituitary-thyroid (HPT) Axis in Fish and Its Role in Fish Development and Reproduction. Critical Reviews in Toxicology, 37(2): 97-115pp.
7. Boas, M. Feldt-Rasmussen, U., Skakkebaek, N.E. and Main, K.M. (2006). Environmental chemicals and thyroid function. European Journal of Endocrinology, 154: 599-611pp.
8. Brouwer, A., Klassonwehler, E., Bokdam, M., Morse, D.C. and Traag, W.A. (1990). Competitive-inhibition of thyroxine binding to transthyretin by monohydroxy metabolites of 3,4,3_,4_- tetrachlorobiphenyl. Chemosphere 20(7–9):1257–1262pp.
9. Brown, D.D. (1997). The role of thyroid hormone in zebrafish and axolotl development. Proc. Natl. Acad. Sci. USA 94: 13011– 13016pp.
10. Brown, S. B. (2004). Contamination effected on the teleoset fish thyroid. Environmental Toxicology Chemical, 23(5): 1680-1701pp.
11. Brown, S.B., Adams, B.A., Cyr, D.G. and Eales, J.G. (2004). Contaminant effects on the teleost fish thyroid. Environmental Toxicology and Chemistry, 23: 1680- 1701pp.
12. Bruker, D.F. 1998. Effects of environmental synthetic chemicals onthyroid function. Thyroid 8: 827–856pp.
13. Burman, K.D. and Wartofsky L. (2001). Endocrine and metabolic dysfunction syndromes in the critically ill: thyroid function in the intensive care unit setting. Crit Care Clin 17: 43-57pp.
14. Byamungu, N., MOL, K. and Kuhn, E.R. )1992(. Evidence for the kidney as an important source of 5’-monodeiodination activity and stimulation by somatostatin in Oreochromis niloticus L. Gen. Comp. Endocrinol. 88: 199-208pp.
15. Carletta, M.A., Weis, P. and Weis, J.S. (2002). Development of thyroid abnormalities in mummichogs, Fundulus heteroclitus, from a polluted site. Marine Environmental Research, 54: 601-4pp.
16. Cooley, H. M., Fisk, A. T., Wiens, S. C., Tomy, G. T., Evans, R. E. and Muir, D. C. G. (2001). Examination of the behavior and liver and thyroid histology of juvenile rainbow trout (Oncorhynchusmykiss) exposed to high dietary concentrations of C 10-, C 11-, C 12-and C 14-polychlorinated n-alkanes. Aquatic Toxicology, 54(1), 81-99pp.
17. Creech, M.M., Arnold, E.V., Boyle, B., Muzinich, M.C., Montville, C., Bohle, D.S. and Atherton, R.W. (1998). Sperm motility enhancement by nitric oxide produced by the oocytes of fathead minnows, Pimephthalespromelas. J. Androl. 19(6):667–674pp.
18. DehghanMadiseh, S., Savary, A., Parham, H. and Sabzalizadeh, S. (2009). Determination of the level of contamination in Khuzestan coastal waters (Northern Persian Gulf) by using an ecological risk index. Environmental Monitoring and Assessment. 159: 521-530pp.
19. De Jesus, E.G. and Hirano, T. (1992). Change in Whole Body Concentration of Cortisol, Thyroid Hormones, and Sex Steroids During Early Development of the Chum Salmon, Oncorhynchus Keta. General and Comparative Endocrinology, 85(1): 55-61pp.
20. Eales, J.G. (1990). Thyroid function in poikilotherms. In: Progress in Comparative Endocrinology. Willy-Liss, Inc. 415-420pp.
21. Eales, J.G., Maclatchy, D.L. and Sweeting, R.M. (1993). Thyroid hormone deiodinase systems in salmonids and their involvement in the regulation of thyroidal status. Fish Physiol. Biochem. 11: 313-321pp.
22. Eales, J.G. and Brown, S.B. (1993). Measurement and regulation of thyroidal status in teleost fish. Reviews in Fish Biology and Fisheries, 3: 299–347pp.
23. Eales, J.G., Brown, S.B., Cyr, D.G., Adams, B.A., and Finnson, K.R. (1999). Deiodination as an index of chemical disruption of thyroid hormone homeostasis and thyroidal status in fish. In: Environmental Toxicology and Risk Assessment: Standardization of Biomarkers for Endocrine Disruption and Environmental Assessment. 8: 1364pp.
24. Eales, J.G. and Brown, S.B. (2005). Thyroid hormones. In Biochemistry and Molecular Biology of Fishes. Vol. 6. Environmental Toxicology, Mommsen, T.P. and Moon, Environmental Bulletin, 6: 355–358pp.
25. Edeline, E., Bardonnet, A., Bolliet, V., Dufour, S. and Pierre, E. (2005). Endocrine control of Anguilla anguilla glass eel dispersal: effect of thyroid hormones on locomotor activity and rheotactic behavior, Horm. Behav. 48. 53–63pp.
26. Fentress, J.A., Brouwer, T.H., Brouwer, M., McLachlan, J.A., and Cheek, A.O. (2000). Examination of a potential environmental antiestrogen, Am. Zool. 40(6):1013–1014pp.
27. Hedayati, S. A. and Arsham, A. (2012). Endocrine Disruption Induced by Sub-Lethal Mercury Chloride on Hormone Indices of Seabream. World J. Fish Mar. Sci, 4: 125-130pp.
28. Hedinger, C., Dillwyn Williams, E. and Sobin, L. H. 1989. The WHO histological classification of thyroid tumors: a commentary on the second edition. Cancer, 63(5), 908-911pp.
29. Hewitt, E.A., Andrew, D., Mark, C., Gunderson, P. (2002). Thyroid status in juvenile alligators (Alligator mississippiensis) from contaminated and reference sites on Lake Okeechobee, Florida, USA. Chemosphere, 47(10): 1129-1235pp.
30. Inui, Y., Yamano, K. and Miwa, S. (1995). The role of Thyroid hormone in tissue development in metamorphosing flonder. Aquaculture, 135(1-3): 87-98pp.
31. Ishihara, A., Sawatsubashi, S., Yamauchi, K. (2003). Endocrine disrupting chemicals: interference of thyroid hormone binding to transthyretins and to thyroid hormone receptors. Molecular and Cellular Endocrinology, 199: 105–117pp.
32. Janz, D.M. (2000). Endocrine system. In: Ostrander, G.K. (Ed.), The Laboratory Fish. Academic Press, SanDiego.
33. Kakkar, K.G. (2011). Water soluble fraction of diesel fuel induced histopathological alterations in the liver of Channa punctatus. Fish and Shellfish Immunology 18: 14-16pp.
34. Kang, D.Y. (2004). Effects of maternal injection of 3,5,30-triidod-Lthyronine (T3) on growth of newborn offspring of rockfish, Sebastesschlegeli, Aquaculture 234 641–655pp.
35. Kavlock, R.J., Daston, C. DeRosa, P., Fenner- Crisp, L.E., Gray, S., Kaattari, G. and Lucier, M. (1996). Luster, M.J. Mac, C. Maczka, R. Miller, J. Moore, R. Rolland, G. Scott, D.M. Sheehan, T. Sinks, and H.A. Tilson. Research needs for the risk assessment of health and environmental effects of endocrine disrupters: a report of the U.S. EPA-sponsored workshop. Environ. Health Perspect. 104 (Supp. 4):715-740pp.
36. Lanni, A., Moreno, M, C., Lombardi, A., and Goglia, F. (1994). Specific binding sites for 3:3´-diiodo-L-thyronine (3, 3´-T2) in rat liver mitochondria. FEBS let, 351:237-240pp.
37. Lema. S.C., Nevitt G.A. (2006). An ecophysiological mechanism for morphological plasticity in pupfish and its relevance to conservation efforts for endangered Devils Hole pupfish, J. Exp. Biol, 209: 3499–3509.
38. Mescher, AL., Junqueira, S. (2010). Basic histology text and atlas. 12 th edition. Philadelphia: McGraw Hill;. p.360-65pp.
39. Matta, S.L.P., Vilela, D.A.R., Godinho, H.P. and Franca L.R. )2002(. The goitrogen 6-n-propyl- 2-thiouracil (PTU) given during testis development increase sertoli germ cell numbers per cyst in fish: the tilapia (Oreochromis niloticus) model, Endocrinology 143. 970–978pp.
40. McCormick, S.D. (2001). Endocrine control of osmoregulation in teleost fish, Integr. Comp. Biol. 41: 781–794pp.
41. Mol, K.A., Van der Geyten, S., Burel, C., K¨uhn, E.R., Boujard, T. and Darras, V.M. (1998). Comparative study of iodothyronineouter ring and inner ring deiodinase activities in five teleostean fishes. Fish.Physiol. Biochem. 18:253–266pp.
42. Morgado, I., Campinho, M. A., Costa, R., Jacinto, R. and Power, D. M. (2009). Disruption of the thyroid system by diethylstilbestrol and ioxynil in the sea bream (Sparus aurata). Aquatic Toxicology, 92(4), 271-280pp.
43. Moriyama, K., Tagami, T., Akamizu, T., Usui, T., Saijo, M., Kanamoto, N., Hataya, Y., Shimatsu, A., Kuzuya., H. and Nakao, K. (2002). Thyroid hormone actionis disrupted by bisphenol A as an antagonist. The Journal of Clinical Endocrinology and Metabolism, 87: 5185–5190pp.
44. Mortoglou, A. and Candiloros, A. (2004). The serum triiodothyronine to thyroxine (T3/T4) ratio in various thyroid disorders and after Levothyroxine replacement therapy. Hormones (Athens) 3: 120-126pp.
45. Morrovati, H. (2000). Marine and coastal biodiversity, ecological survey By‌ Mhrganhem and Bhrgan.University of Chamranmartyrand EPA province. 201p.
46. Plisetskaya, E., Woo., N.Y.S. and Murat., J.C. (1983).Thyroid hormones in cyclostomes and 9*and Physiology .Part A: Physiol, 74:179–187pp.
47. Nabavi, S.M.B. (2002).Environmental indicators and approaches to improve crisis in Khoor Moosa. Iran's first national conference on environmental crises and ways of enhancing them.
48. NajafiAsfad, M. (2011). Assessment the oil pollution despite international conventions to prevent their removal. Journal of Mazandaran University of Medical Sciences. 21 period. 94-101pp.
49. Naji, T. Safaeian, Sh., Rostami, M. And Sabrjo, M. (2007). Evaluation of the effects of zinc on gill common carp (Cyprinuscarpio). Environmental Science and Technology, Volume 9, Issue 2, 36-29pp.
50. NeginTaji, A., Archangi, B., Movahedinia, A., Safahieh, A.R. and Eskandari, Gh. (2013). micronucleus use of the hormone as early biomarkers of exposure to contaminants substance Bisphenol A in Yellow fin Sea bream (Acanthopagrus latus), the journal Oceanography. 16 number. 23-32pp.
51. Pratima, G., Chaurasia, S.S., Anand, K., Maiti, P.K., Gupta, P., Kar, A. (1997). Influence of cadmium on thyroid hormone concentrations and lipid peroxidation in a fresh water fish, Clariasbatrachus. Fresenius Environmental Bulletin, 6: 355–358pp.
52. Ram, R. N. and Sathyanesan, A. G. (1987). Histopathological changes in liver and thyroid of the teleost fish, Channa punctatus (Bloch), in response to ammonium sulfate fertilizer treatment. Ecotoxicology and environmental safety, 13(2), 185-190pp.
53. Roland, R. M. (2000). A review of chemically-induced altrations in thyroid and vitamin a status from field studies of wildlife and fish. Journal Wild Disease. 36:615-635pp.
54. Rousset, B. and Mornex, R. (1991). The thyroid hormone secretory pathway-current dogmas and alternative hypotheses. Molec. Cell. Endocrinol. 78: 89-93pp.
55. Safahieh, A., Monikh, F. A., Savari, A., Doraghi, A. and Ronagh, M. T. (2014). Spatial and seasonal variations of heavy metal concentration in sediment, Musa estuary (Persian Gulf). Indian Journal of Geo-Marine Sciences, 43(5), 849-857pp.
56. Satari, M., Shahsavani, D., Shafiei, Sh. (1383). Fish Biology 2 (Systematic). Hagh Shenas publication.Tehran, Iran.
57. Scott, GR., Sloman, K.A. (2004). The effects of environmental pollutants on complex fish behaviour: integrating behavioural and physiological indicators of toxicity. Aquat To5xicol 68:369–392pp.
58. Sheirani, M.(2011). Assayactive enzymes as biomarkers of oil pollution in mudskipper (Periophthalamus waltoni) off the coast of Iran (Persian Gulf) animal environment. S2.
59. Soleimani, Z. Salamat, N. Safahieh, A.R. Savari, A. Ronagh, M.(2011). Study of pathological changes ofthe gills and all the fish of Liza abu (Liza abu) and yellow fin sea bream (Acanthopagrus latus) affected by pollution Mahshahr creeks, master's thesis. Khorramshahr University of Marine Science and Technology. 86p.
60. Takagi, Y., Hirno, T., Tanabe, H and Yamada, J. (1994). Stimulation of Skeletal growth3 by thyroid hormone administration in the rainbow trout, Oncorhynchus mykiss. Journal of Experimental Zoology, 268(3): 229-238. regulation of hypothalamic pituitary-thyroid (HPT) axispp.
61. Teles, M., Pacheco, M and Santos, M.A. (2007). Endocrine and metabolic responses of Anguilla anguillaL. caged in a freshwater-wetland (Pateira de Fermentelos- Portugal). Science of the Total Environment, 372: 562-570pp.
62. Thangavel, P., Sumathiral, K., Karthikeyan, S. and Ramaswamy, M. (2005). Endocrine response of the freshwater teleost, Sarotherodon mossambicus (Peters) to dimecron exposure. Chemosphere, Vol. 61, 1083-92 pp.
63. Ucer, B. (2011). Analyzing dependence structure of thyroid hormones: a copula approach. Journal of Medical Sciences, 41(4):725-34pp.
64. Vaboniyan, A., Movahedinia, A. (2014). Effect of concentrations the cadmiumon thyroid hormones Yellow fin Seabream (Acanthopagrus latus), the journal Oceanography, Issue19, 27-34pp.
65. Zhou, T., John Alder, HB. and Weis, P. (2002). Endocrine disruption : thyroid dysfunction in mummichoge (Fundulus heteroclitus) from a polluted habitat. Marine Environmental Research, 50 (1-5): 393-397pp.
66. Zoeller, R.T., Tan, S.W. (2007). Implications of research on assaysto characterize thyroid toxicants. Crit. Rev. Toxicol. 37,195–210pp.