بررسی تاثیر مزمن آلاینده بوتاکلر برروی شاخص‌های آسیب‌شناسی بافت کلیه و عضله در ماهی قرمز (Carassius auratus)

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی کارشناسی ارشد - دانشگاه مازندران

2 گروه زیست شناسی دریا، دانشکده علوم دریایی، دانشگاه مازندران، بابلسر، ایران

3 دکتری بوم شناسی دریا، گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه گیلان

چکیده

بررسی آسیب‌ها و تغییرات بافت شناختی به منظور تخمین تاثیرپذیری موجودات زنده از مواد و آلاینده‌های شیمیایی اهمیت زیادی برخوردار است. این تحقیق به منظور ارزیابی تغییرات هیستوپاتولوژیکی عضله و کلیه ماهی حوض goldfish (Carassius auratus) در مواجهه با آلاینده آلی بوتاکلر در سال 1393 به اجرا در آمده است. بدین منظور 32 قطعه ماهی گلد فیش بالغ تهیه و در 4 آکواریوم(8 قطعه ماهی در هر آکواریوم) در معرض سه غلظت زیرکشنده آلاینده بوتاکلر 60 درصد به میزان 1/0، 14/0 و 28/0 میلی‌لیتر بر لیتر و یک آکواریوم شاهد به مدت دو هفته، قرار گرفتند. سپس، از هر تیمار 6 قطعه ماهی به صورت تصادفی انتخاب شد، بخش میانی هر دو کلیه و نیز بافت عضله از بخش زیرین باله پشتی آن‌ها جدا شد. بافت‌ها در محلول بوئن فیکس شد و پس از طی مراحل بافت شناسی، برش های 6 میکرونی تهیه و با روش هماتوکسیلین_ائوزین رنگ‌آمیزی شد. یافته‌های بافتی در این پژوهش دال‌بر تغییر پاتولوژیکی نظیر افزایش مراکز ملانوماکروفاژ، چروکیدگی گلومرول‌ها و اتساع فضای بومن، کاهش یا انسداد فضای ادراری، اتساع مویرگ‌های گلومرولی، ضخیم شدن غشاء پایه گلومرولی، دژنرسانس سلول‌های ادراری و جدا شدن سلول‌ها از غشاء پایه، نفوذ لکوسیت‌ها، خونریزی و نکروز لوله‌های ادراری در کلیه بود. همچنین با افزایش غلظت بوتاکلر در تیمارهای مختلف وسعت و شدت ضایعات افزایش نشان داد. یعلاوه تغییر پاتولوژیکی بافتی از قبیل تغییرات در خطوط، هسته‌، تورم ابری، دژنرسانس هیالین، دژنر سانس دانه‌ای و نکروز در بافت عضلانی مشاهده شد که بیشترین شدت تخریب در غلظت 28/0 میلی لیتر بر لیتر بود.

کلیدواژه‌ها


عنوان مقاله [English]

The effects of butachlor on indicators of pathological contaminants chronic kidney tissue and muscle in goldfish (Carassius auratus)

نویسندگان [English]

  • M. Zarei 1
  • Hassan Taghavi 2
  • F. Nazarhaghighi 3
1 Marine Ecology Graduate Student, Faculty of Marine Biology Sciences, University of Mazandaran.
2 Faculty of Marine Biology, College of Marine Sciences, University of Mazandaran, Babolsar, Mazandaran.
3 Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran.
چکیده [English]

In order to determine the damage and histological alteration, evaluation of the effects of materials and chemical contaminants on organisms, is important. The present study reports an overall assessment of histopathological changes in kidney and muscle of goldfish in exposure to Butachlor. The experiment was carried out in1393. For this purpose, 32 adult fish supply and 4 aquariums (8 fish per tank) were exposed to different concentrations of pollutants: sub-lethal concentrations of 0.1, 0.14 and 0.28 ml butachlor 60% per liter and a control aquarium for two weeks. Then, 6 pieces was randomly selected from each treatment, the center of both kidneies and muscle tissues were removed from the bottom of the dorsal fin. At the end of the exposing period, the muscles below the dorsal fin were removed and fixed by bouins solution, processed, sectioned at 6µ thickness and further stained by Hematoxylin-Eosin general method. Histological findings in this study indicated pathological changes such as increase in Melanomacrophage centers, glomeruli shrinkage and Bowman's space expansion, reduction or blockage of the urinary tract, glomerular capillary dilation, thickening of the glomerular basement membrane, degeneration of urinary cells and separation from cell membrane, permeation of leukocytes, bleeding of urinary tubules in kidney and necrosis. Increasing the concentrations of butachlor in different treatments led to the increased extent and severity of lesions. Based on the histological study, histopathological changes in muscle, such as changes in striation, nuclear changes, cloudy swelling, hyaline degeneration, granular degeneration and necrosis were observed. The most damage was observed at the concentration of 0.28 ml/l. 

کلیدواژه‌ها [English]

  • Tissue damage
  • Goldfish
  • Sublethal concentration
  • Kidney
  • muscle

1-Abo Nour,A. Amer, A. 1995. Impairment of muscle performance in the Nile catfish Clarias lazera in response to hostathion insecticide contamination and/or gamma irradiation. Journal of the. Egyptian.German. Society of Zoology. 18, 153-175.
2-Ada, F.B. Ndome, C.B. Bayim, P.R. 2011. Some haematological changes in Oreochromis niloticus juveniles exposed to butachlor. Journal of agriculture and food technology, 1, 73–80.
3-Adams, S.M. 2002 Biological indicators of aquatic ecosystem stress: introduction and overview. In: Adams, S.M. Biological indicators of aquatic ecosystem stress, American Fisheries Society, Bethesda, 1-12.
4-Adeogun, A.O., Chukwuka, A.V. and Ibor, O.R. 2011. Impact of abattoir and saw-mill effluents on water quality of upper Ogun River (Abeokuta). American Journal of Environmental Sciences. 7: 525–530.
5-Ahmadivand, A. Farahmand, H. Mirvaghefi, A.R, Eagderi, S., Shokrpoor, S and Rahmati-Holasoo, H. 2014. Histopathological and haematological response of male rainbow trout (Oncorhynchus mykiss) subjected to Butachlor. Veterinarni Medicina. 59: 433–439.
6-Dass, B. and Mukherjee, S. 2000. A histopathological study of carp (Labeo rohita) exposed to hexachlorocyclohexane. Vet. Arhiv. 70: 169-180.
7-Dezfuli B.S., Simoni. E., Giani, L. and Mormera,M. 2006.Effects of experimental terbuthylazine exposure on the cells of Dicertrarchus labrax.Chemosphere. 64: 1684.1694.
8- El-Serafy, S.S, Ibrahim, S.A. and Mahmoud,S.A. 2005. Biochmical and histopathological studies on the muscles of the Nile Tilapia (Oreochromis niloticus) in Egypt. Egyptian Journal of Aquatic Biology & Fisheries. 9: 81–96.
9-Esmaily Sari, A., 2002, Pollutants, Health and Standards in the Environment, 1st Edn.,Mehr Pub., Iran.
10-Gernhofer, M., Pawert, M., Schramm, M., Muller, E., and Triebskorn, R.2001.Ultrastructural Biomarkers As Tools To Characterize The Health Status Of Fish In Contaminated Streams, Journal Of Aquatic Ecosystem Stress And Recovery (Formerly Journal Of Aquatic Ecosystem Health) 8, 241-260.
11-Giari, L., Simoni, E., Manera, M., and Dezfuli, B.S. 2008. Histocytological responses of Dicentrarchus labrax (L.) following mercury exposure. Ecotoxicol. Environ Safety. 70: 400-410.
12-Guo H.R, Yin L.C, Zhang S.C, Feng W.R. 2011. The toxic mechanism of high lethality of herbicide butachlor in marine flatfish flounder, Paralichthys olivaceus. Journal of Ocean University of China. 9: 257–264.
13-Hample, M.,Ortiz-Delgado, J.B., Sarasquete, C. and Blasco, J. 2008. Effects of sediment sorbed linear alkylbenzene sulphonate on juveniles of the Senegal sole, Solea senegalensis: toxicity and histological indicators. Histology and histopathology, 23: 87-100.
14- Haya, K. 1989. Toxicity of pyrethroid insecticides to fish. Environ. Toxicol. Chem, 8: 381–391.
15-Hinton, D.E., Baumann, P.C., Gardner, G., Hawkins, W.E., Hendricks, J.D. and Okihiro, M.S. 1992. Histologic biomarkers. In: Huggett R.J., Kimerli R.A., Mehrle Jr. P.M. and Bergman H.L. (Eds). Biomarkers: biochemical, physiological and histological markers of anthropogenic stress. Lewis publishers, boca raton. USA. 156.196.
16-Karuppasamy, R. 2000. Tissue histopathology of channa punctatus(Bloch) under phenyl mercuric acetate toxicity. Bulletin of pure and Applied Sciences., 19:109-116.
17-Khan,R.A. 1995. Histopathology in winter flounder, Pleuronectes americanus,following chronic exposure to crude oil. Bulletin of environmental contamination and toxicology, 54,279-301.
18-Movahedinia, A. Loghmani, M.Ghasemi,S. Kouchaknejad, E. Izadian, M. Esfandiari, E. 2014. Study on the effects of Benzo(a)pyrene exposure on renal tissues in Common 16- Carp, Cyprinus carpio. Aquatic Physiology and Biotechnology. 2:31-45. [in Farsi]
19-Nasci, C., Da Ros, L., Campesan, G., Van Vleet, E.S., Salizzato, M., Sperni, L., and Pavoni, B. 1999. Clam transplantation and stress related biomarkers as useful tools for assessing water quality in coastal environments. Mar. Pollut. Bull., 39: 255-260.
20-Oliveira, R.C.A., Fanta, E., Turcatti, N.M., Cardoso, R.J., and Carvalho, C.S. 1996. Lethal effects of inorganic mercury on cells and tissues of Trichomycterus Brasiliensis. Biocell. 20,: 171-178.
21-Ortiz, J.B., De Canales, M.L.G. and Sarasquete, C. 2003. Histopathological changes induced by lindane (gamma-HCH) in various organs of fishes. Sci. Mar., 67: 53–61.
22-Posti,M. Sadigh,M. 1994.An Atlas of fish histology normal and pathological feature. Tehran University Press p.781 [in Farsi]
23-Ramah, K. 2011. Histopathological study on the effect of rice herbicides on grass carp (Ctenopharyngodan idella). African Journal of biotechnology.,10,: 1112-1116.
24-Roncarati.A, Melott. P, Dee .A, Mordenti .O and Angellotti, L 2006. Welfare status of cultured seabass (Dicentrarchus labrax L) and seabream (Sparus aurata L.) assessed by blood parameters and tissue characteristics. International Aquatic Research. 22,: 225–234.
25-Sakr, S and Gabr S. 1991. Ultrastructural changes induced by diazinon and neopybuthrin in skeletal muscles of Tilapia nilotica. Proceed. Zool. Soc. A.R.E., 21: 1-14.
26-Sitohy, M.Z. El-Masry, R.A. Siliem, T.A. and Mohamed, N.A. 2006. Impact of some trace metals pollution in the River Nile water on muscles of Claries gariepinus inhabiting El-Kanater El-Khyria and Helwan sites. Zagazig Journal of Agricultural Research, 33: 1207–1222.
27-Takashima, F and Hibiya, T. 1995. An atlas of fish histology: normal and pathological features. Lubrecht & Cramer Ltd. P: 213.
28-Teh, S.J. Adams, S. and Hinton, D.E. 1997. Histopathological biomarkers in feral freshwater fish populations exposed to different types of contaminant stress. Aquat. Toxicol., 37: 51-70.
29-Tilak, K.S. Satyavardhan, K and Thathaji, P.B. 2003. Biochemical changes induced by fenvalerate in the freshwater fish Channa punctata. J. Ecotoxicol. Environ. Monit., 13,: 261-270.
30-Tilak, K.S. Veeraiah, K. Thathaji, P.B and Butchiram MS. 2007. Toxicity studies of butachlor to fresh water fish, Channa punctata (Bloch). Journal of Environmental Biology. 28:485–487.
31-Tilak, K.S. Veeraiah. K and Koteswara. D. 2004. Toxicity and bioaccumulation of chlorpyrifos in Indian carp Catla catla (Ham.), Labeo rohita (Ham.) and Cirrhinus mrigala (Ham). Bull. Environ. Contam Toxicol., 3,: 933-941.
32-Venkatesam, R and Subramaian. N. 2007. Effect of copper sulphat onhistopathological changes in the fresh water fish Oreochromis mossambicus (peters).J.Ecotoxi., 17.: 353-361.
33-Yokote, M. 1982. Digestive system, In Hibiya T (ed.): An Atlas of Fish Histology: Normal and Pathological Features. Kodansha Ltd, Tokyo, p. 74–93.
34-Young, A.L. 1987. Minimising the risk aociated with peticides minimizing the risk. ragsdale R. J. kuhr (Eds). ACS symp. ser. 336 Amer.chem. soc.Wahington. D. C.
35-Zarei,M. Salamat,N. Nabavi,MB. Fahieh,A. Ghanemi,K . 2013. In vitro effects of Benzo[a]Pyrene different concentrations on tissue structure of kidney in Orange-spotted Grouper (Epinephelus coioides). Journal of food microbiology. 4:1-10. [in Farsi]
36- Das, B. and Mukherjee. S, 2000. A histopathological study of carp (Labeo rohita) exposed to hexachlorocyclohexane. Veterinary Archives, 70:169 - 180.
37- Mohamed, F.A.S. and. Gad, N.S. 2008. Environmental Pollution - Induced biochemical changes in tissues of T. zilli, S. vulagris and M. capito from Lake Qarun, Egypt. Global Veterinaria., 2(6), 327 – 336.
38- Mohamed, F.A.S. 2009. Histopathological studies on Tilapia zilli and Solea vulgaris from Lake Qarun, Egypt. World Journal of Fish and Marine Science. 1, 29 - 39 (2009).
39- Bhuvaneshwari, R. Padmanaban, K. and BabuRajendran, R. 2015. HistopathologicalAlterations inMuscle, Liver and GillTissues of Zebra Fish Danio Rerio due to Environmentally Relevant Concentrations of Organochlo Rine Pesticides (OCPs) and Heavy Metals. International Journal of Environmental Research. 9(4):1365-1372.
40- Ateeq B, Farah MA, Ahmed W 2006: Evidence of apoptotic effects of 2, 4-D and butachlor on walking catfish, Clarias batrachus, by transmission electron microscopy and DNA degradation studies. Life Sciences 78, 977–986.
41- Yadav, A. S. Bhatnagar, A. and Kaur, M. 2010. Assessment of Genotoxic Effects of Butachlor in Fresh Water Fish, Cirrhinus mrigala (Hamilton). Research Journal of Environmental Toxicology, 4: 223-230.
42- Nwani, C. D., Ama, U. I., Okoh, F., Oji, U. O., Ogbonyealu, R. C., Agha, A., Ibiam and Udu-Ibiam, O. 2013. Acute toxicity of the chloroacetanilide herbicide butachlor and its effects on the behavior of the freshwater fish Tilapia zillii. African Journal of Biotechnology Vol. 12(5), pp. 499-503.
43- Patnaik, B. B., Howrelia, H., Mathews, T., and Selvanayagam, M. 2011. Histopathology of gill, liver, muscle and brain of Cyprinus carpio communis L. exposed to sublethal concentration of lead and cadmium. African Journal of Biotechnology. 10(57): pp. 12218-12223.