اثرات سطوح مختلف لاکتوفرین جیره بر آنزیم‌های گوارشی، ترکیب بیوشیمیایی لاشه و فلور باکتریایی روده بچه‌ماهی صبیتی (Sparidentex hasta)

نوع مقاله : مقاله کامل

نویسندگان

1 دانشجوی دکترای پژوهشکده مطالعات دریاچه ارومیه، دانشگاه ارومیه و کارشناس پژوهشکده خلیج فارس، دانشگاه خلیج فارس، بوشهر، ایران

2 دانشیار گروه شیلات پژوهشکده مطالعات دریاچه ارومیه، دانشگاه ارومیه، ایران

3 دانشیار پژوهشی پژوهشکده آبزی پروری جنوب کشور، اهواز، ایران

4 استادیار گروه شیلات پژوهشکده مطالعات دریاچه ارومیه، دانشگاه ارومیه، ایران

5 استادیار گروه بهداشت آبزیان دانشکده دامپزشکی، دانشگاه شهید چمران، اهواز، ایران

چکیده

هدف از این مطالعه، تعیین اثرات سطوح مختلف لاکتوفرین جیره بر روی فعالیت آنزیم‌های گوارشی، ترکیبات بیوشیمیایی لاشه و فلور باکتریایی روده بچه ماهی صبیتی (Sparidentex hasta) با میانگین وزنی 3/0 ± 64/7 گرم بود. این مطالعه در قالب یک طرح کاملا تصادفی با 3 تیمار و 3 تکرار در تانک‌های فایبرگلاس 300 لیتری انجام شد. ماهیان با جیره‌های حاوی 0، 400 و 800 میلی گرم لاکتوفرین در هر کیلوگرم غذا به مدت 42 روز تغذیه شدند. در پایان آزمایش نمونه‌های لاشه و روده جمع‌آوری شد. نتایج حاصل نشان داد که لاکتوفرین جیره فعالیت آنزیم‌های گوارشی شامل پروتئاز، آمیلاز و لیپاز ماهی صبیتی را تغییر نداد (05/0 < p). در این آزمایش رابطه همبستگی ضعیف، مثبت و غیر معنی‌داری بین لاکتوفرین جیره و فعالیت آنزیم پروتئاز، آمیلاز و لیپاز مشاهده شد (05/0 < p). نتایج این مطالعه نشان داد که سطوح مختلف لاکتوفرین تاثیری بر روی ترکیب بیوشیمیایی بدن شامل پروتئین، خاکستر و رطوبت و فلور باکتریایی روده ندارد (05/0 < p) ، اما میزان چربی لاشه در ماهیان تغذیه شده با 400 میلی گرم لاکتوفرین در هر کیلوگرم غذا به صورت معنی‌داری بالاتر از گروه شاهد بود (05/0 > p). به طور کلی، این مطالعه نشان داد که فعالیت آنزیم‌های گوارشی به وسیله لاکتوفرین جیره تحت تاثیر قرار نمی‌گیرد. علاوه بر این، می توان نتیجه‌گیری کرد که تغذیه ماهی صبیتی با جیره حاوی 400 و 800 میلی گرم لاکتوفرین در هر کیلوگرم غذا برای یک دوره 6 هفته ای ترکیب بیوشیمیایی بدن و فلور باکتریایی روده را بهبود نمی‌بخشد.

کلیدواژه‌ها

عنوان مقاله [English]

Effects of different levels of dietary lactoferrin on digestive enzymes, body composition and intestine bacterial flora of sobaity (Sparidentex hasta) fingerling

نویسندگان [English]

  • V. Morshedi 1
  • N. Agh 2
  • J. Marammazi 3
  • F. Noori 4
  • T. Mohamadian 5
1 PhD student of Urmia Lake Research Institute, Urmia University, Urmia and Researcher of Persian Gulf Institute, University of Persian Gulf, Bushehr, Iran.
2 Associate professor of Department of Fisheries of Urmia Lake Research Institute, Urmia University, Urmia, Iran.
3 Associate professor of South Iranian Aquaculture Research Center, Ahwaz, Iran.
4 Assistant professor of Department of Fisheries of Urmia Lake Research Institute, Urmia University, Urmia, Iran.
5 Assistant professor of Department of Aquatic Health of Faculty of Veterinary, Shahid Chamran University, Iran.
چکیده [English]

The aim of this study was to determine the effects of dietary lactoferrin (LF) on digestive enzymes activity, body composition and intestine bacterial flora of sobaity (Sparidentex hasta) with an average weight of 7.64 ± 0.3 g. This study was carried out in a completely randomized design with three treatments and replications in fiberglass tanks with 300 liters volume. Fish were fed with feed containing 0, 400 and 800 mg lactoferrin per kg feed for 42 days. At the end of the experiment, body composition and intestine samples were collected. The obtained results indicated that dietary lactoferrin did not change sobaity digestive enzymes activity, including protease, amylse and lipase (P > 0.05). In this study, weak, positive and no significant correlation were observed between dietary lactoferrin and protease activity, amylase activity, and lipase activity (P > 0.05). The results indicated that different levels of lactoferrin did not affect body composition including protein, ash and moisture and intestine bacterial flora (P > 0.05) but fat content in fish fed on 400 mg lactoferrin per kg feed was significantly higher that control group (P < 0.05). Overall, this study showed that digestive enzymes activity was not affected by dietary lactoferrin. Moreover, it can be concluded that feeding of sobaity on the diet supplemented with 400 and 800 mg lactoferrin per kg feed for a period of 6 weeks do not improve the body composition and intestine bacterial flora.

کلیدواژه‌ها [English]

  • Lactoferrin
  • Digestive enzymes
  • Body composition
  • intestine bacterial flora
  • Sparidentex hasta

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تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
دوره 29، شماره 4
دی 1395
صفحه 65-74

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سابقه مقاله

  • تاریخ دریافت: 09 آذر 1394
  • تاریخ بازنگری: 16 شهریور 1395
  • تاریخ پذیرش: 12 بهمن 1394

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