عنوان مقاله [English]
The aim of this study was to determine the effects of dietary lactoferrin (LF) on digestive enzymes activity, body composition and intestine bacterial flora of sobaity (Sparidentex hasta) with an average weight of 7.64 ± 0.3 g. This study was carried out in a completely randomized design with three treatments and replications in fiberglass tanks with 300 liters volume. Fish were fed with feed containing 0, 400 and 800 mg lactoferrin per kg feed for 42 days. At the end of the experiment, body composition and intestine samples were collected. The obtained results indicated that dietary lactoferrin did not change sobaity digestive enzymes activity, including protease, amylse and lipase (P > 0.05). In this study, weak, positive and no significant correlation were observed between dietary lactoferrin and protease activity, amylase activity, and lipase activity (P > 0.05). The results indicated that different levels of lactoferrin did not affect body composition including protein, ash and moisture and intestine bacterial flora (P > 0.05) but fat content in fish fed on 400 mg lactoferrin per kg feed was significantly higher that control group (P < 0.05). Overall, this study showed that digestive enzymes activity was not affected by dietary lactoferrin. Moreover, it can be concluded that feeding of sobaity on the diet supplemented with 400 and 800 mg lactoferrin per kg feed for a period of 6 weeks do not improve the body composition and intestine bacterial flora.
1. Abdel-Tawwab M., Abdel-Rahman M. A., Nahla-Ismael E. M. (2008). Evaluation of commercial live bakers’ yeast, Saccharomyces cerevisiae as a growth and immunity promoter for Fry Nile tilapia, Oreochromis niloticus (L.) challenged in situ with Aeromonas hydrophila. Aquaculture, 280: 185–189.
2. Ahmadian E. (2012). Effects of different levels of dietary lactoferrin on egg quality of rainbow trout (Oncorhynchus mykiss) broodstocks. Thesis of Master of Science, Urmia University, 93 p (In Persian).
3. Al-Mashikhi ShA., Nahai Sh. (1987). Isolation of bovine immunoglobulins and lactoferrin from whey proteins by gel filtration technique. Journal of Dairy Science, 70(12) : 2486-2492.
4. Andani H. R. R., Tukmechi A., Meshkini S., Sheikhzadeh N.(2012). Antagonistic activity of two potential probiotic bacteria from fish intestines and investigation of their effects on growth performance and immune response in rainbow trout (Oncorhynchus mykiss). Journal of Applied Ichthyology, 28: 728–734.
5. AOAC. (2000). Association of Official Analytical Chemists, 16th (end), Procedure 984. 25.
6. Askarian F., Matinfar A., Kousha A., Bahmani M., Khorshidi K., Shenavar A., Ringo E. (2008). Diversity of lactic acid bacteria in the gastro intestinal tracts of reared Beluga (Huso huso) and Persian sturgeon (Asipenser persicus). Journal of Fisheries and Aquatic Science, 3(5) : 302-311.
7. Bahram Beigi M. (2013). Study of using synbiotic Lactobacillus plantarum probiotic andxylo oligosaccharidesprebiotic on growth indices, digestive enzymes activity, immune responses and environmental shocks in rainbow trout (Oncorhynchus mykiss). Thesis of Master of Science, Urmia University. 107 p (In Persian).
8. Bernfeld P. (1955). Amylase. In: Colowick, S.P., Kaplan, N.O (Eds) , Methods in Enzymology. Academic Press, New York. Pp. 149-158.
9. Bradford M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry, 72: 248-254.
10. Brock J. H. (2002). The physiology of lactoferrin. Biochemistry and Cell Biology, 80: 1-6.
11. El-Sayed Badawy T., Al-Kenawy D. (2013). Assessment of immune response supplemental immunoton and bovine lactoferrin as alternatives to antibiotics in Nile Tilapia (Oreochromis niloticus). Journal of the Arabian Aquaculture Society, 8(2) : 341-355.
12. Falahatkar B., Soltani M., Abtahi B., Kalbassi M. R., Pourkazemi M. (2006). Effects of dietary vitamin C supplementation on performance, tissue chemical composition and alkaline phosphatase activity in beluga sturgeon (Huso huso). Journal of Applied Ichthyology, 22: 283-286.
13. FAO. (2015). FishStat Plus datasets. Fishery statistical collections: Aquaculture Production (1950–2013; released March 2015). Rome, Italy: Food and Agriculture Organization of the United Nations.
14. Garcia-Carreno F. L., Haard N. F. (1993). Characterization of proteinase classes in Langostilla pleuroncodes planipes and Crayfish Pacifastacus astacus extracts. Journal of Food Biochemistry, 17: 97-113.
15. Garcia-Carreno F. L., Albuquerque-Cavalcanti C., del Toro M. A. N., Zaniboni-Filho E. (2002). Digestive proteinase of Brycon orbignyanus (Characidae, Teleostei): characteristics and effects of protein quality. Comparative Biochemistry and Physiology B, 132: 343–352.
16. Hagi T., Tanaka D., Iwamura Y., Hoshino T. (2004). Diversity and seasonal changes in lactic acid bacteria in intestinal tract of cultured fish. Aquaculture, 234: 335-346.
17. Heidarieh M., Mirvaghefi A. R., Akbari M., Farahmand H., Sheikhzadeh N., Shahbazfar A. A., Behgar M. (2012). Effect of dietary Ergosan on growth performance, digestive enzymes, intestinal histology, hematological parameters and body composition of rainbow trout (Oncorhynchus mykiss). Fish Physiology and Biochemistry, 38: 1169–1174.
18. Hentges D. J., Marsh W. W., Petschow B. W., Thal W. R., Carter M. K. (1992). Influence of infant diets on the ecology of the intestinal tract of human flora-associated mice. Journal of Pediatric Gastroenterology and Nutrition, 14: 146–152.
19. Hidalgo M. C., Urea A., Sanz A. (1999). Comparative study of digestive enzymes in fish with different nutritional habits. Proteolytic and amylase activities. Aquaculture, 170: 267-283.
20. Iijima N., Tanaka S., Ota Y. (1998). Purification and characterization of bile salt-activated lipase from hepatopancrease of red sea bream Pagarus major. Fish Physiology and Biochemistry, 18: 59-69.
21. Jenssen H., Hancock, B. R. (2009). Antimicrobial properties of lactoferrin. Biochimie, 91: 19-29.
22. Kumari J., Swain T., Sahoo P. K. (2003). Dietary bovine lactoferrin induces changes in immunity level and disease resistance in Asian catfish, Clarias batrachus. Veterinary Immunology and Immunopathology, 94: 1-9.
23. Lopez-Vasquez K., Castro-Perez C. A., Val A. L. (2009). Digestive enzymes of eight Amazonian teleosts with different feeding habits. Journal of Fish Biology, 74: 1620-1628.
24. Moraiti-Ioannidou M., Castritsi-Catharios J., Miliou H. (2008). Biochemical composition and digestive enzyme activity during naupliar development of Artemia spp. from three solar saltworks in Greece. Aquaculture, 286: 259-265.
25. Nya E. J., Austin B. (2011). Dietary modulation of digestive enzymes by the administration of feed addetives to rainbow trout Onchorhynchus mykiss Walbaum. Aquaculture Nutrition, 17: 495-466.
26. Ringo E., Gatesoupe F. J. (1998). Lactic acid bacteria in fish: a review. Aquaculture, 160: 177-203.
27. Safarpour Amlashi A., Falahatkar B., Sattari M., Tolouei Gilani M. H. (2011). Effect of dietary vitamin E on growth, muscle composition, hematological and immunological parameters of sub-yearling beluga Huso huso L. Fish and Shellfish Immunology, 30: 807-814.
28. Sakai M. (1999). Current research status of fish immunostimulants. Aquaculture, 172: 63-92.
29. Salam A., Davies P. M. C. (1994). Body composition of northern pike, Esox lucius L., in relation to body size and condition factor. Journal of Fisheries Research, 19: 193-204.
30. Skrodenyte-Arbaciauskiene V., Sruoga A., Butkauskas D., Skrupskelis K. (2008). Phylogenetic analysis of intestinal bacteria of freshwater salmon Salmo salar and sea trout Salmo trutta trutta and diet. Fisheries Science, 74: 1307–1314.
31. Soleimani N., Hoseinifar S. H., Merrifield D. L., Barati M., Hassan Abadi Z. (2012). Dietary supplementation of fructooligosaccharide (FOS) improves the innate immune response, stress resistance, digestive enzyme activities and growth performance of Caspian roach (Rutilus rutilus) fry. Fish and Shellfish Immunology, 32: 316-321.
32. Sun Y. Z., Yang H. L., Ma R. L., Song K., Li J. S. (2011). Effect of Lactococcus lactis and Enterococcus faecium on growth performance, digestive enzymes and immune response of grouper Epinephelus coioides. Aquaculture Nutrition, 165: 1-9.
33. Sunde J., Taranger G., Rungruangsak-Torrissen K. (2001). Digestive protease activities and free amino acids in white muscle as indicators for feed conversion efficiency and growth rate in Atlantic salmon (Salmo salar L.). Fish Physiology and Biochemistry, 25: 335-345.
34. Suzer C., Çoban D., Kamaci H. O., Saka S., Firat K., Otgucuoģlu Ö. (2008). Lactobacillus spp. Bacteria as probiotics in gilthead sea bream (Sparus aurata L.) larvae: effects on growth performance and digestive enzyme activities. Aquaculture, 280, 140-145.
35. Teraguchi S., Ozawa K., Yasuda S., Shin K., Fukuwatari Y., Shimamura S. (1994). The bacteriostatic effects of orally administered bovine lactoferrin on intestinal Enterobacteriaceae of SPF mice fed bovine milk. Bioscience, Biotechnology, and Biochemistry, 58: 482–487.
36. Wakabayashi H., Yamauchi K., Takase M. (2006). Lactoferrin research, technology and applications. International Dairy Journal, 16: 1241-1251.
37. Wang Y. B., Xu Z. (2006). Effect of probiotics for common carp (Cyprinus carpio) based on growth performance and digestive enzyme activities. Animal Feed Science and Technology, 127: 283-292.
38. Xu B., Wang Y., Li J., Lin Q. (2009). Effect of prebiotic xylooligosaccharides on growth performances and digestive enzyme activities of allogynogenetic crucian carp (Carassius auratus Gibelio). Fish Physiology and Biochemistry, 35: 351–357.
39. Ye J. D., Wang K., Li F. D., Sun Y. Z. (2011). Single or combined effects of fructo- and mannan oligosaccharide supplements and Bacillus clausii on the growth, feed utilization, body composition, digestive enzyme activity, innate immune response and lipid metabolism of the Japanese flounder Paralichthys olivaceus. Aquaculture Nutrition, 17: 902-911.