عنوان مقاله [English]
Mycoplasmosis is an important pathogen of poultry and turkeys which causes economic losses in poultry industry. The aim of this study was to investigate the seroprevalance of Mycoplasma gallisepticum (MG) infection and to identify some effective factors related with it in broiler chicken flocks in Ardabil province of northwestern Iran during 2012 to 2013. To achieve this, 20 blood samples were randomly taken from each 36 broiler flocks which were in the end of farming period in slaughterhouses.Samples were immediately sent to laboratory and after serum isolation from samples, Rapid Serum Agglutination test (RSA) and ELISA for investigation of infection rate was performed. From total 720 samples, in RSA 30.3% and in ELISA 19.7% of samples were MG positive. Sensitivity, specificity and correlation rate of RSA in compassion to ELISA, were 100%, 88.4% and -0.546, respectively. In view of MG seroprevalance in different seasons, the highest rate of seroprevalance with statistically difference (p<0.001) was in Winter. The highest rate of MG seroprevalance was in hens and large flocks (≥20001birds) and the lowest was in male and small flocks (≤5000 birds). In comparison of MG seroprevalance in various geographical regions, the dense area of Namin was statistically different (p<0.001) and had highest infection rate. The results of this study showed that contamination rate of Mycoplasma gallisepticum is relatively high in industrial flocks. Thus observation of sanitation facts, biosecurity, capacity and standard density in control of disease is very important.
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