بررسی اثرات ضد باکتریائی باکتریهای Lactobacillus casei و Lactobacillus acidophilus روی باکتری Paenibacillus larvae عامل لوک آمریکائی زنبورعسل در شرایط آزمایشگاه

نوع مقاله: مقاله کامل

نویسندگان

1 استادیار موسسه تحقیقات واکسن و سرم‌سازی رازی، کرج، ایران

2 دانشیار گروه میکروبیولوژی دانشکده دامپزشکی ارومیه

چکیده

در این بررسی با استفاده از روش‌های Agar spot test و انتشار در چاهک و Microdilution اثرات ضد باکتریائی لاکتوباسیلوس کازئی و لاکتوباسیلوس اسیدوفیلوس روی باکتری پنی باسیلوس لاروا عامل لوک آمریکائی زنبورعسل مورد مطالعه قرار گرفت. در روش Agar spot test غلظت cfu/ml 107 باکتری لاکتوباسیلوس کازئی و لاکتوباسیلوس اسیدوفیلوس به ترتیب باعث ایجاد هاله ممانعت از رشد باکتری به قطر 45 میلی متر و 40 میلی متر علیه باکتری پنی باسیلوس لاروا گردیدند. در روش انتشار در چاهک، 100 میکرولیتر از مایع روئی عاری از سلول(cfcs)، باکتری لاکتوباسیلوس کازئی و باکتری لاکتوباسیلوس اسیدوفیلوس به ترتیب باعث ایجاد هاله ممانعت از رشد باکتری به قطر 40 و 30 میلی متر و 50 میکرولیتر مایع روئی باکتری لاکتوباسیلوس کازئی و باکتری لاکتوباسیلوس اسیدوفیلوس به ترتیب باعث ایجاد هاله ممانعت از رشد 30 و 25 میلی متر و 30 میکرولیتر مایع روئی باکتری لاکتوباسیلوس کازئی و باکتری لاکتوباسیلوس اسیدوفیلوس به ترتیب باعث ایجاد هاله ممانعت از رشد 20 و 12 میلی‌متر شدند. در روش میکرودیلوشن غلظت‌های cfu/ml 108 الی 103 هر دو باکتری اسید لاکتیک مانع از رشد باکتری پنی‌باسیلوس لاروا در غلظت cfu/ml 104 گردیدند. ولی هر دو باکتری در غلظت cfu/ml 102 تأثیری روی باکتری مذکور نداشتند. لذا غلظت cfu/ml 103 هر دو باکتری اسیدلاکتیک استفاده شده به عنوان حداقل غلظت مهار کننده رشد(MIC) و حداقل غلظت کشندگی(MBC) هر دو باکتری علیه باکتری پنی‌باسیلوس لاروا در نظر گرفته شدند. نتایج این بررسی نشان داد که باکتری‌های لاکتوباسیلوس کازئی و لاکتوباسیلوس اسیدوفیلوس باعث مهار رشد باکتری پنی باسیلوس لاروا عامل بیماری لوک آمریکائی زنبورعسل در محیط کشت شده و با اثراتی که روی جوانه زدن اسپور باکتری مذکور و همچنین نابودی شکل رویشی آن می‌گردند می‌توانند در صورت بی خطری آن‌ها برای لاروهای زنبورعسل، به عنوان یکی از روش‌های مبارزه با این بیماری در کلنی‌های زنبورعسل مورد استفاده قرار گیرند.

کلیدواژه‌ها


عنوان مقاله [English]

Antibacterial effects of Lactobacillus casei and Lactobacillus acidophilus on the Paenibacillus larvae causative agent of honeybee American foulbrood disease In vitro

نویسندگان [English]

  • M. Moradi 1
  • A. Ownagh 2
1 Assistant Professor Razi Vaccine and Serum Research Institute, Karaj, Iran.
2 Assosiate Professro Group Microbiology Facoulty of Oroumiah
چکیده [English]

In this survey antibacterial effects of Lactobacillus casei and Lactobacillus acidophilus against Painibacillus larvae, causative agent of honeybee American foulbrood disease, has investigated by Agar spot test, Well diffusion and Microdilution methods. In Agar spot test 107cfu/ml of Lactobacillus casei and Lactobacillus acidophilus made inhibition zone 45 and 40 mm around spots on the agar medium respectively. In the well diffusion method, 100 microliters of cell free supernatant (cfcs) of Lactobacillus casei and Lactobacillus acidophilus mede inhibition zone 40 and 30 mm around wells respectively, 50 microlitrs of L. casei and L. acidophilus supernatant mede inhibition zone 30 and 25 mm around wells respectively and 30 microlitrs of L. casei and L. acidophilus supernatant made inhibition zone 20 and 12 mm around wells respectively. In the microdulation method 103 -108cfu/ml concentrations of two lactobacillus inhibited P. lrvae growth completely, but 102 and 10 cfu/ml of two lactobacillus have not any effect on the P. larvae growth. The results of this study show that L. casei and L. acidophilus have antibacterial effects on the P. larvae, causative agant of the honeybee American foulbrood disease and can use as a safe remedy for treatment of this disease.

کلیدواژه‌ها [English]

  • honeybee
  • American foulbrood disease
  • Paenibacillus larvae Lactobacillus casei
  • Lactobacillus acidophilus

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