بررسی اثر کاشت ملاتونین بر عملکرد غده فوق کلیه میش نژاد عربی در خارج از فصل تولیدمثلی

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی کارشناسی ارشد فیزیولوژی دام دانشگاه کشاورزی و منابع طبیعی رامین خوزستان

2 دانشیار گروه علوم دامی دانشگاه کشاورزی و منابع طبیعی رامین خوزستان

3 استادیار گروه علوم دامی دانشگاه کشاورزی و منابع طبیعی رامین خوزستان

4 استاد دانشکده دامپزشکی دانشگاه شهید چمران اهواز

چکیده

هدف از این تحقیق، بررسی اثر ایمپلنت ملاتونین بر عملکرد غده فوق کلیه و میزان هورمون کورتیزول سرم خون در خارج از فصل تولیدمثلی میش‌های نژاد عربی بود. بدین منظور 16 رأس میش عربی 2 تا 4 ساله (با میانگین وزن 5±35 کیلوگرم) غیرآبستن به دو گروه آزمایشی تقسیم شدند. این تحقیق از اوایل دی ماه تا اواسط اسفند که مصادف با فصل غیرتولیدمثلی میش عربی می‌باشد، انجام شد و طول دوره‌ی آزمایش 10 هفته بود. در گروه اول (شاهد) هیچ گونه درمانی صورت نگرفت. در گروه دوم از کاشت ملاتونین استفاده شد. وزن غده فوق کلیه و ضخامت نواحی قشر، کپسول، گلومرولوزا، فاسیکولاتا و رتیکولاریس این غده در تیمار کاشت ملاتونین نسبت به تیمار شاهد به طور معنی‌داری کاهش یافت (01/0 > p). اختلاف آماری معنی‌داری در میزان کورتیزول سرم خون بین دو تیمار شاهد و کاشت ملاتونین در تمامی زمان‌های مورد مطالعه وجود نداشت (05/0 < p). در گروه شاهد، میزان هورمون کورتیزول سرم خون در روز 14 پس از کاشت ملاتونین بطور معنی‌داری بیشتر از روزهای 42 و 56 بود (01/0 > p). اما در گروه تیمار بین زمان‌های مختلف تفاوت معنی‌داری مشاهده نشد  (05/0 < p). طبق نتایج حاصل، استفاده از ملاتونین جهت القاء فصل تولیدمثلی در میش عربی، یک عامل استرس زا محسوب نمی‌شود و کاربرد آن موجب افزایش میزان کورتیزول نمی گردد

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of melatonin implant on adrenal gland function in Arabian ewe in out of breeding season

نویسندگان [English]

  • Kianfar, M. 1
  • M. Mamouei, 2
  • S. Tabatabaei-Vakili, 3
  • Kh. Mirzadeh, 3
  • N. Erfani-Majd, 4
1 M.Sc. student of Animal Physiology, Ramin Agriculture and Natural Resources University of Khuzestan.
2 Associated Professor, Department of Animal Sience, Ramin Agriculture and Natural Resources University of Khuzestan.
3 Assistant Professors, Department of Animal Sience, Ramin Agriculture and Natural Resources University of Khuzestan.
4 Professor, Faculty of Veterinary Medicine, Shahid Chamran University of Ahwaz.
چکیده [English]

The purpose of this study was to investigate the effect of  melatonin implant on adrenal function and serum cortisol level in Arabian ewes out of the breeding season. For this purpose, 16 Arabian ewes with 2 to 4 years old (mean weight 35±5 kg) and non-pregnant were divided into two groups. The survey was conducted from late December to early March for 10 weeks. The first group (control) received no melatonin. Implantation of melatonin was used in the second group. Weight of adrenal gland in treatment of melatonin implant in compare with control group is significantly dicrease (p<0.01). Weight of adrenal gland and thickness of the cortex, capsule, glomerulosa, fasciculate and reticularis in treatment of melatonin implant has been decreased in compare with control group (p<0.01). In all times of study, there was no significant differences in cortisol levels between melatonin and control treatments (P>0.05). in control group, the cortisol concentration of blood serum in day 14 was significantly higher than days 42 and 56 (p<0.01). But in melatonin treatment, there were no significant different in cortisol value among all times of study (P>0.05). With regard to the results of this study can be said, use of melatonin to induction breeding season in Arabian ewes, not as a stressor and its application in out of the breeding season did not increase the cortisol level. 

کلیدواژه‌ها [English]

  • Adrenal gland
  • Arabian ewe
  • Cortisol
  • Melatonin
  • Serum

1- Chemineau, P., Malpaux, B., Pelletiet, J., Leboeuf, B., Delgadillo, J.A., Deletang, F., et al.(1996). Use of melatonin implants and photoperiodic treatments to control seasonal reproduction in sheep and goats. Journal of Production Animals. 9: 45-60.
2- De Souza, C.J. and Meier, A.H. (1987). Circadian and seasonal variations of plasma insulin and cortisol concentrations in the Syrian hamster, Mesocricetusauratus.Chronobiology International.4:141-151.
3- Gundogan, M. (2006). Some reproductive parameters and seminal plasma constituents in relation to season in Akkaraman and Awassi rams. Turish Journal of Veterinary and Animal Sciences 30: 95-100.
4- Guyton, A.C. and Hall, J.E. (2011). Textbook of medical physiology. 12th ed.,Saunders
5 - Hatami, M., Rahimi, G.H., Rumizadeh, E., Babaei, M., Kamju, B. and Aghabitalab, H. A. (2010). Detection of polymorphism in MTNR1A gene using PCR-RFLP in Zandi sheep breed. The 4th Congress on Animal Science.
6 - Hazlerigg, D.G. (2001). What is the role of melatonin within the anterior pituitary? Journal of Endocrinology. 170:493-501.
7- Keegan, C.E. and Hammer, G.D. (2002). Recent insights into organogenesis of the adrenal cortex. Trends Endocrinology Metabolism. 13:200-208.
8- Laliotis, V., Vosniakou, A., Zafrakas, A., Lymberopoulos, A. and Alifakiotis, T. (1998). The effect of melatonin on lambing and litter size in milking ewes after advancing the breeding season with progestagen and PMSG followed by artificial insemination. Small Ruminant Research.31:79-81.
9- Markova, M., Adamekova, E., Kubatka, P., Bojkova, B., ahlersova, E. and Ahlers, I. (2003). Effect of Prolonged Melatonin Administration on Metabolic Parameters and OrganWeights In Young Male and Female Sprague-Dawley Rats. ActaVeterinaria Brno. 72:163–173.
10- Marteniuk, J.V. and Herdt, T.H. (1988). Pregnancy toxemia and ketosis of ewes and does. Veterinary Clinics of North America: Food Animal Practice. 4: 307-15.
11- Misztal, T., Romanowicz, K. and Barcikowski, B. (2004). Effect of melatonin on luteinizing hormone secretion in anestrous ewes following dopamine and opiate receptor blockade. Animal Reproduction Science. 81:245-259.
12- Mousavi, S.M. and Sookhtezary, A. (2011). Effect of melatonin on the reproductive progestagens of sheep in non-reproductive season. Iranian Journal of Animal Science Research. 3(1):84-88.
13- Mohammadghasemi, F., Faghani, M. and Khagejahromi, S. (2011). The protective effect of melatoninon the test is of adult micetreated with busulfan. Journal of Reproduction and Infertility.11(2): 67-76.
14- Notter, D.R. and Chemineau, P. (2001). Nocturnal melatonin and prolactin plasma concentrations in sheep selected for fertility in autumn lambing. Journal of Animal Science. 79: 2895-2901.
15- Ottenweller, J.E., Tapp, W.N., Pitman, D.L. and Natelson, B.H. (1987). Adrenal, thyroid, and testicular hormone rhythms in male golden hamsters on long and short days. American Journal of Physiology.253: 321-328.
16- Parker, L.N., Levin, E.R. and Lifrak, E.T. (1985). Evidence for adrenocortical adaptation to sever illness. Journal of Clinical Endocrinology and Metabolism. 60(5): 947-952.
17- Pertsov, S.S. (2006). Effect of melatonin on the thymus, adrenal glands, and spleen in rats during acute stress.Bulletin of Experimental Biology and Medicine journal.141(3):292-295.
18- Steensberg, A., Fischer, C.P. and Sacchetti, M. (2003). Acuteinterleukin-6 administration does not impair muscleglucose uptake or whole-body glucose disposal in healthy humans. The Journal of Physiology. 548(2):631–8.

19- Stutz , G., Zamudio, J., Santillán, M.E., Vincenti, L., de Cuneo, M.F. and Ruiz, R.D. (2004). The effect of alcohol, tobacco, and aspirin consumption on seminal quality among healthy young men. Archives of Environmental Health.59(11):548-52.
20- Wang, M., Yokotani, K., Nakamura, K., Murakami, Y., Okada, S. and Osumi, Y. (1999). Melatonin inhibits the central sympatho-adrenomedullary outflow in rats. Japanese Journal of Pharmacology 81: 28– 33.
21- Yamada, K. (1990). Effects of melatonin on adrenal function in male rats. Research Communications in Chemical Pathology and Pharmacology. 69(2):241-4.
22- Yoshiki, H., Yuko,S. and Matsushima, S. (2001). Effects of photoperiod and melatonin on the development of growth hormone cells and the pituitary-adrenal axis in the Djungarian hamster,Phodopussungorus.Archives of Histology and Cytology.64(2): 211-222.