تاثیر روش‌های مختلف تجویز Lactobacillus rhamnosus در هچری بر عملکرد رشد، مورفولوژی روده و فاکتورهای خونی جوجه‌های گوشتی با آلودگی تجربی سالمونلا


1 دانشجوی دکترای تخصصی دانشگاه تربیت مدرس

2 عضو هیأت علمی دانشگاه تربیت مدرس


به منظور بررسی تاثیر روش‌های مختلف تجویز Lactobacillus rhamnosus در هچری بر عملکرد رشد، مورفولوژی و فاکتورهای خونی تعداد 225 قطعه جوجه گوشتی سویه راس 308 در پنج گروه شامل شاهد و چهار روش تجویز به صورت تزریق داخل تخم ‌مرغ، دهانی، افشانه و کلواکی با 3 تکرار و 15 قطعه جوجه در هر واحد بررسی شدند. یک روز بعد از تجویز پروبیوتیک‌ها با روش‌های مختلف تمام پرندگان با CFU/ml  107 Salmonella entritidis از طریق گاواژ دهانی چالش داده شدند. مطالعه عملکرد رشد و مورفولوژی روده کوچک به صورت هفتگی صورت گرفت. نمونه‌گیری از خون به منظور بررسی برخی فاکتورهای خونی در 21 روزگی انجام شد. بیشترین میانگین افزایش وزن روزانه و کمترین ضریب تبدیل مربوط به گروه تلقیح در کلواک بود (05/0>P). روش‌های تجویز اثر معنی‌داری بر مورفولوژی روده باریک داشتند. ارتفاع پرز در ابتدای روده باریک در روش تزریق داخل تخم‌مرغ از سایر گروه‌ها بالاتر بود که تفاوت معنی‌داری با روش افشانه نداشت (01/ 0>P). بیشترین ارتفاع پرز در ایلئوم مربوط به گروه تلقیح کلواکی بود (01/0>P). روش‌های مختلف تجویز بر غلظت کلسترول تاثیر معنی‌داری داشتند و بیشترین میزان کاهش کلسترول، HDL و LDL در گروه تلقیح کلواکی مشاهده شد(05/0>P).

عنوان مقاله [English]

Effect of different routes of Lactobacillus rhamnosus administration in hatchery on performance, intestine morphology and serum cholesterol in experimentally salmonella infected broiler chickens.

نویسندگان [English]

  • Z Hashemzadeh 1
  • M.A Karimi Torchizi 2
  • SH Rahimi 2
1 Msc Graduated of Tarbiat Modares University
2 Scientific Members of Tarbiat Modares University
چکیده [English]

The effects of various routes of probiotic administration on performance, small intestine morphology and serum cholesterol of broiler chickens, were studied. We assigned 225 day old chicks (Ross 308) to five experimental groups including control and four hatchery administration methods comprised of in ovo injection, oral gavage, spray application and cloacal inoculation. All of these were challenged by 107 Log CFU/ml Se using oral gavage one day after administration of probiotics.  Each treatment had three replications of 15 chicks. Performance and intestine morphology were studied on weekly basis. Concentration of cholesterol, HDL and LDL in serum was determined at day 21. The highest daily body weight gain and the less feed conversion coefficient was observed in cloacal administered group (P<0.05). Significant effect was observed on length of villi in all 3 segments of small intestine among different routes applied for probiotic administration. In ovo injection resulted in significant improvement in length of villi in proximal segments of small intestine, duodenum and jejunum, compared to that of in the other groups except for spray group (P<0.01). Length of villi in distal segment of small intestine, ileum, was higher in cloacal administered group compared to that of in the other groups (P<0.01). Significant effect was observed on serum lipids among different routes of probiotic administration and the less content of cholesterol, HDL and LDL was observed in cloacal administered group (P<0.05).   

1- تشفام، ا.، رحیمی، ش. و کریمی، ک. (1384) تاثیر سطوح مختلف پروبیوتیک بر مورفولوژی مخاط روده جوجه‌های گوشتی. مجله دانشکده دامپزشکی دانشگاه تهران.60: 211-205.##

2-Abdulrahim, S.M., Haddadin, M.S.Y., Hashlamoun, E.A.R. and Robinson, R.K. (1996) The influence of Lactobacillus acidophilus and bacitracin on layer performance of chickens and cholesterol content of plasma and egg yolk. British Poultry Science. 37:341-346.##

3-Ahmad, I. (2006) Effect of probiotics on broilers performance. International Journal of Poultry Science. 5:593-597.##

4-Alami Abardeh, J. (1994) Investigation the intestinal villi morphology of clinical coccidiosis infected chickens. University of Tehran. DVM Thesis. No. 2224.##

5-Angel, C.R. (1991) Long segmented filamentous organism observed in poults experimentally infected with stunting syndrome agent. Avian Diseases. 34:994-1001. ##

6-Arun, K, P., Savaram, V. R. R, Mantena, V.L.N. and Sita, R. S. (2006) Dietary supplementation of Lactobacillus sporogenes on performance and serum biochemico-lipid profile of broiler chickens. The Journal of Poultry Science. 43:235-240. ##

7-Baba, E., Nagaishi, S., Fukata, T. and Arakawa, A. (1991) The role of intestinal microflora on the prevention of Salmonella colonization on gnotobiotic chickens. Poultry Science. 70:1902–1907.##

8-Barrow, P. A. (1992) Probiotics for chickens. R. Fuller. ed. Chapman and Hall,  London, pp:224-257.##

9-Bradley, G.L., Savage, T.F. and Timm, K.I. (1994) The effects of supplementing diets with Saccharomyces cerevisiae var. boulardi on male poult performance and ileal morphology. Poultry Science. 73:1766-1770.##

10-Choudhury, K., Das, J., Saikia, S., Sengupta, S. and Choudhury, S.K. (1998) Supplementation of broiler diets with antibiotic and probiotic fed muga silk worm pupae meal. Indian Journal of Poultry Science. 33:339-342.##

11-Coates, M.E. and Fuller, R. (1977) The gnoto animal in the study of gut microbiology. In: R.T.J. Clarke and T. Bauchop (Eds). Microbial Ecology of the Gut. Academic Press. London, p: 311-346.##

12-Corrier, D.E., Hollister, A.G., Nisbet, D.J., Scanlan, C.M., Beier, R.C. and Deloach, J.R. (1994) Competitive exclusion of Salmonella Enteritidis in leghorn chicks: Comparison of treatment by crop gavage, drinking water, spray, or lyophilized alginate beads. Avian Diseases. 38:297-303.##

13-Cox, N.A., Bailey, J.S., Mauldin, J.M. and Blankenship, L.C. (1990) Presence and impact of Salmonella contamination in commercial broiler hatcheries. Poultry Science. 69:1606–1609.##

14-Edens, F.W., Parkhurst, C.R., Casas, I.A. and Dobrogosz, W.J., (1997) Principles of ex ovo competitive exclusion and in ovo administration of Lactobacillus reuteri. Poultry Science. 76:179-196.##

15-Fukushima, M. and Nakano, M. (1995) The effect of probiotic on faecal and liver lipid classes in rats. British Journal of Nutrition. 73:701-710.##

16-Fuller, R. (1989) A review: Probiotics in man and animals. Journal of Applied Bacteriology. 66:365-378.##

17-Ghadban, G.S. (2002) Probiotics in broiler production- a review. Archiv für Geflügelkunde. 22:49-58.##

18-Gotto, A.M. and Farmer, J.A. (1992) Risk factors for coronary artery disease In: Heart disease. A textbook of cardiovascular medicine. Chapter, 37. Volume, 2. Brounwald, E. ed. W. B. Saunders Company, Philadelphia. PA.##

19-Ichikawa, H., T. Kuroiwa, A. Inagaki, R. Shineha, T. Philips, I. (1999) Assessing the evidence that antibiotic, probiotic bacteria stimulate gut epithelial cell proliferation in rat. Digestive Diseases and Sciences. 44:2119-2123.##

20-Isshiki, Y. (1979) Effect of Lactobacilli in the diet on the concentration of nitrogenous compounds and mineral blood of chicken. Japanese Poultry Science. 16:254-258.##

21-Jin, L.Z., Ho, Y.W., Abdullah, M.A. and Jalaludin, S. (1998)Growth performance, intestinal microbial populations, and serum cholesterol of broilers fed diets containing lactobacillus cultures. Poultry Science. 77:1259-1265.##

22-Joy, A.D. and Samuel, J.J. (1997) Effect of probiotic supplementation on the performance of broilers. Journal Veterinary and Animal Sciences. 28:10-14.##

23-Kalavathy, R., Abdullah, N., Jalaludin, S. and Ho, Y.W. (2003) Effects of Lactobacillus cultures on growth performance, abdominal fat deposition, serum lipids and weight of organs of broiler chickens. British Poultry Science. 44:139-144.##

24-Kannan, M., Karunakaran, R., Balakrishnan, V. and Prabhakar, T.G. (2005) Influence of probiotics supplementation on lipid profile of broilers. International Journal of Poultry Science. 4:994-997.##

25-Karimi Torshizi, M. A., Rahimi, Sh., Mojgani, N., Esmaeilkhanian, S. and Grimes, J. L. (2008) Screening of indigenous strains of lactic acid bacteria for development of a probiotic for poultry. Asian Australasian Journal of Animal Science. 21(10):1495-1500.##

26-Klaver, F.A.M. and van der Meer, R. (1993) The assumed assimilation of cholesterol by Lactobacilli and Bifidobacterium bifidum is due to their bile salt deconjugating activity. Applied and Environmental Microbiology. 59:1120-1124.##

27-Lepkovsky, S., Wanger, M., Furuta, F., Ozine, K. and Koike, T. (1964) The protease, amylase and lipase of the pancreas and intestinal contents of germfree and conventional chicken. Poultry Science. 43:722-735. ##

28-Mead, G.C. (2000) Prospects for competitive exclusion treatment to control Salmonellas and other food borne pathogens in poultry. Veterinary Journal. 159:111-123. ##

29-Meijerhof, R. and Hulet, R.M. (1997) In ovo injection of competitive exclusion culture in broiler hatching eggs. Journal of Applied Poultry Research. 6:260-266.##

30-Mohan, B., Kadirvel, R., Natarajan, A. and Bhaskaran, M. (1996) Effect of probiotic supplementation on growth, nitrogen utilization and serum cholesterol in broilers. British Poultry Science. 37:395-401.##

31-Mountzouris, K., Beneas, H., Tsirtsikos, P., Kalamara, E. and Fegeros, K. (2006) Evaluation of the effect of a new probiotic product on broiler performance and cecal microflora composition and metabolic activities. International Poultry Scientific Forum Atlanta, Georgia January 23–24.##

32-Mouwen, J.M.V.M. (1971) White scours in piglets at three weeks of age. Veterinary Pathology. 8:364-380.## 

33-Murry, A.C., Hinton, A. and Buhr, R.J. (2006) Effect of botanical probiotic containing lactobacilli on growth performance and populations of bacteria in the ceca, cloaca, and carcass rinse of broiler chickens. International Journal of Poultry Science. 5:344-350.##

34-Nelson, C.R. and Gilliland, S.E. (1984). Cholesterol uptake by Lactobacillus acidophilus. Journal of Dairy Science. 67:115-120.##

35-Nishihira, S., Satomi, A. and Sakata, T. (1999) Probiotic bacteria stimulate gut epithelial cell proliferation in rat. Digestive Diseases and Sciences. 44:2119-2123.##

36-NRC, (1994) Nutrient requirements of poultry. 9th revised ed. National Academy Press. Washington, DC.##

37-Panda, A.K., Reddy, M.R. and Praharaj, N.K. (2001) Dietary supplementation of probiotic on growth, serum cholesterol and gut microflora of broilers. Indian Journal of Animal Science. 71:488-490.##

38-Pelicano, E.R.L., Souza, P.A. Souza, H.B.A., Figueiredo, D.F., Boiago, M.M., Carvalho, S.R. and Bordon, V.F. (2005) Intestinal mucosa development in broiler chickens fed natural growth promoters. Brazilian Journal of Poultry Science. 7:221-229.##

39-Pivnick, H. and Nurmi, E. (1982) The Nurmi concept and its role in the control of Salmonella in poultry. In: R. Davis(Ed.). Developments in food microbiology. 1. Applied Science Publishers. London, pp. 41-70.##

40-Richmond, W. (1973) Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum. Clinical Chemistry. 19:1350-1356.##

41-Rolfe, R.E., (2000) The role of probiotic cultures in the control of gastrointestinal health. Journal of Nutrition. 130:396-402. ##

42-Samanya, M. and Yamauchi, K. (2002) Histological alterations of intestinal villi in chickens fed dried Bacillus subtilis var. natto. Comparative Biochemistry and Physiology. Part A. 133:95–104.##

43-Santin, E., Maiorka, A., Macari, M., Grecco, M., Sanchez, J.C., Okada, T.M. and Myasaka, A.M. (2001) Performance and intestinal mucosa development of broiler chickens fed diets containing Saccharomyces cerevisiae cell wall. Journal of Applied Poultry Research. 10:236-244.##

44-SAS Institute, (1990) SAS/STAT® User's guide, release 6.03 edition. SAS Institute Inc., Cary, NC.##

45-Songsak, C., Orawan, C., Thassawan, S., Sathit, N. and Sirilak, S. (2008) Effect of dietary inclusion of cassava yeast as probiotic source on growth performance, small intestine (ileum) morphology and carcass characteristic in broilers. International Journal of Poultry Science. 7:246-250.##

46-Takahashi, S.E., Mendes, A.A., Saldanha, E.S.P.B., Pizzolante, C.C., Pelícia K., Quinteiro, R.R., Komiyama, C.M., Garcia, R.G. and Almeida Paz, I.C.L. (2005) Efficiency of prebiotics and probiotics on the performance, yield, meat quality, and presence of Salmonella spp in carcasses of free-range. Brazilian Journal of Poultry Science. 7:151-157.##

47-Van Leeuwen, P., Mouwen, J.M.V.M., Van Der Klis, J.D. and Verstegen, M.W.A. (2004) Morphology of the small intestinal mucosal surface of broilers in relation to age, diet formulation, small intestinal micro flora and performance. British Poultry Science. 45:41-48.##

48-Zhang, A.W., Lee, B.D., Lee, S.K., Lee, ., K.W.,  An, G.H.,  Song, K.B.  and Lee. C.H. (2005) Effects of yeast (Saccharomyces cerevisiae) cell components on growth performance, meat quality and ileal mucosa development of broiler chicks. Poultry Science. 84:1015-1021.##

49-Zulkifli, I., Abdullah, N., Azrin, N. M. and Ho, Y. W. (2000) Growth performance and immune response of two commercial broiler strains fed diets containing Lactobacillus cultures and oxytetracycline under heat stress conditions. British Poultry Science. 41:593–597.##