بررسی عملکرد و سیستم ایمنی هومورال (تیتر پادتن در مقابل واکسن گامبورو، غلظت سرولوپلاسمین و نسبت هتروفیل به لنفوسیت) جوجه‌های گوشتی تغذیه شده با سطوح مختلف پروبیوتیک و روغن سویا

نویسندگان

1 دانش آموخته گروه علوم دامی، دانشکده کشاورزی، دانشگاه ارومیه

2 دانشیار گروه علوم دامی، دانشکده کشاورزی، دانشگاه ارومیه

3 استادیار گروه علوم دامی، دانشکده کشاورزی، دانشگاه ارومیه

چکیده

در این تحقیق اثرات افزودن سطوح مختلف روغن سویا (2، 4 و 6 درصد جیره) و پروبیوتیک پریمالاک (صفر، 1/0 و 15/0 درصد جیره) در فاصله‌ی سنی 7 تا 42 روزگی بر عملکرد و سیستم ایمنی جوجه‌های گوشتی مورد بررسی قرار گرفت. برای این منظور از540 قطعه جوجه‌ی نر یکروزه‌ی سویه‌ی کاپ در 9 گروه 60 تایی استفاده شد و هر گروه در 6 قفس 10 تایی قرار گرفتند و 6 تکرار (قفس) به هر یک از تیمارهای آزمایشی اختصاص پیدا کردند. تیمارهای آزمایشی در قالب یک آزمایش فاکتوریل 3‌×‌3 بر پایه‌ی طرح کاملاً تصادفی مورد بررسی قرار گرفتند. همه‌ی پرندگان یک جیره‌ی مشابه آغازین را تا سن 7 روزگی دریافت کردند ولی بعد از آن با جیره های آزمایشی رشد (از 7 تا 21 روزگی) و پایانی (از 22 تا 42 روزگی) دارای سطوح متفاوت پروبیوتیک و روغن سویا تغذیه شدند. نتایج حاصله نشان داد که استفاده از روغن سویا یا پروبیوتیک باعث بهبود مقدار مصرف خوراک، افزایش وزن و ضریب تبدیل خوراکی شد (01/0 >P)، بعلاوه یک اثر متقابل معنی‌دار بین مصرف پروبیوتیک و روغن سویا برای ضریب تبدیل خوراک مشاهده شد (05/0 >P). افزودن پروبیوتیک یا روغن سویا منجر به افزایش مصرف خوراک در 24 ساعت بعد از تزریق واکسن و تیتر پادتن تولید شده در مقابل واکسن گامبورو شد در حالی که کاهش غلظت سرولوپلاسمین پلاسما و نسبت هتروفیل به لنفوسیت خون را باعث گردیدند (01/0 >P). بعلاوه یک اثر منقابل معنی‌داری بین روغن سویا و پروبیوتیک برای  مصرف خوراک و تیتر پادتن تولید شده مشاهده شد (05/0 >P). هیچ کدام از تیمارهای آزمایشی تاْثیر معنی‌داری بر وزن نسبی بورس فابرسیوس نداشتند اما افزودن پروبیوتیک به طور معنی‌داری وزن نسبی طحال را در سن 42 روزگی افزایش داد. به طور کلی استفاده از 1/0 درصد پروبیوتیک همراه با 4 درصد روغن سویا بهترین تاْثیر را بر عملکرد جوجه‌های گوشتی نشان داد، اما بهترین پاسخ سیستم ایمنی (غلظت پایین تر سرولوپلاسمین و نسبت کمتر هتروفیل به لنفوسیت خون) با مصرف هم زمان سطوح 1/0 یا 15/0 درصد پروبیوتیک و سطح بالاتر روغن سویا (6 درصد) به دست آمد.

عنوان مقاله [English]

Evaluation of performance and humeral immune system (antibody titer against Gambaro vaccine, cerloplasmin concentration and hetrophyl to lymphocyte ratio) in broiler chickens fed different levels of probiotic

نویسندگان [English]

  • M Nayebpour 1
  • P Farhoomand 2
  • M Daneshyar 3
1 Msc, Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran
2 Associate Professor, Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran
3 Assistant Professor, Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran
چکیده [English]

The effects of different dietary levels of soybean oil (2, 4 and 6%) and probiotic primalac (0, 0.1 and 0.15 %) were investigated on performance and immune system of broiler chickens from day one to day 42 of age. For this purpose, five hundred and forty day-old male broiler chicks (Cobb) in 9 groups of 60 each were used. The birds of each group were divided into 6 pens of 10 birds. The experimental treatments were analyzed in a 3×3 factorial experiment with a completely randomized design all the birds received a same starter diet until day 7 of age but fed the grower (from day 7 to 21 of age) and finisher (from day 22 to day 42 of age) diets afterwards. The results of the recent experiment showed that soybean oil or probiotic supplementation improved the feed consumption, body weight gain and feed conversion ratio (p< 0.01) of broilers during whole the experimental period. Moreover a significant interaction was observed between the probiotic and soybean oil for feed conversion ratio (p< 0.05). Probiotic or soybean oil supplementation increased the feed consumption during 24 hours post-vaccination and antibody titer against gamboro vaccine and decreased the plasma cerloplasmin and hetrophil to lymphocyte ratio (p< 0.01). Furthermore a significant interaction was observed between the soybean oil and probiotic supplementation for post vaccination feed consumption and produced antibody titer (p< 0.05). None of the treatments affected the proportional weight of bursa fabrciuos but probiotic supplementation increased the proportional weight of spleen at day 42 of age. It was concluded that dietary supplementation of 4% soybean oil with 0.1% of probiotic has the best effect on performance of broiler chickens but higher levels of soybean oil (6%) with either of 0.1 or 0.15% of  probiotic is needed for the best immune response (lower blood cerloplasmin concentration and heterophil to lymphocyte ratio). 

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