شمارش میکروارگانیسم های هوازی مزوفیل و جداسازی باکتری سالمونلا از تخم مرغ های مصرفی شهر اصفهان در سال 1389

نویسندگان

1 عضو هیات علمی مرکز آموزش جهاد کشاورزی اصفهان

2 گروه میکروبیولوژی دانشکده دامپزشکی دانشگاه تهران

3 گروه بیماری های طیور دانشکده دامپزشکی دانشگاه تهران

چکیده

133 عدد تخم مرغ از سطح مغازه های شهر اصفهان جمع آوری و به روش استاندارد مورد آزمایش شمارش میکروارگانیسم های هوازی مزوفیل (Total count) قرار گرفتند. همچنین بر روی 186 عدد تخم مرغ اقدام به جداسازی باکتری سالمونلا گردید. نتایج نشان داد میزان آلودگی تخم مرغ ها از  3000 کلنی در گرم تا 10  میلیون متغیر بود. متوسط آلودگی تخم مرغ ها 1693000 کلنی در گرم بر آورد شد. محاسبه آماری با دقت 95 درصد و انحراف معیار 232/2 نشان داد که 7 درصد از تخم مرغ ها بار میکروبی نزدیک میانگین، 68 درصد کمتر از میانگین و 25  درصد بالاتر از میانگین جامعه دارند. از 3 نمونه (61/1 درصد) باکتری entritidis .S  جدا گردید. که در آزمایش PCR دو نمو نه (07/1 درصد) entritidis .S مورد تائید قرار گرفت.

عنوان مقاله [English]

The count of aerobic mesophill bacteria and isolate salmonella Spp on egg in Isfahan1389

نویسندگان [English]

  • H Miranzadeh 1
  • T Zahrei Salehi 2
  • V Karimi 3
1 Institute of Scientific Applied Higher Education of Jahad – e – Agriculture, Center of Isfahan
2 Microbiology Department, Faculty of Vet. Med.,Tehran University
3 Poultry Disease Department, Faculty of Vet. Med. Tehran University
چکیده [English]

This Survey was Conducted on 133 egge that they were collected from Isphahan markets, Eggs are tested for aerobic mesophill bacteria (Total count) and 186 eggs were tested for salmonella spp. contamination. The results showed 3000 to 10 million colony per gram bacteria counted in egg samples. The mean colony count was 1693000.7 percentage samples rich to mean colony count. (95% accuracy,SD2.232)the total count rate were 68 percentage under average and 25 percentage upper average. Salmonella entritidis was isolated from 3eggs samples. among 3 cases salmonella confirmed in 2 cases by PCR.

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