کاربردهای روش اتودیسپلی در بیان پروتئین‌های نوترکیب روی سطح سلولی باکتری‌ها

نویسنده

موسسه تحقیقات واکسن و سرم سازی رازی

چکیده

سیستم های ترشح پروتئین در همه موجودات زنده ازجمله در باکتری های گرم منفی و اندامک های یوکاریوتیک منشعب از آنها وجود دارد. بر خلاف سایر موجودات زنده، باکتری های گرم منفی از تعدادی سیستم های ترشح پروتئین مستقل از یکدیگر برخوردارند. حداقل چهار تیپ سیستم ترشحی که انتقال پروتئین به خارج از غشاء سیتو پلاسمی یا قرار دادن پروتئین در غشاء سیتو پلاسمی را عهده دارند، در باکتری های گرم منفی وجود دارد. مقایسه سیستم های متفاوت باکتریائی با یکدیگر پیشنهاد میکند که علیرغم تشابهات بیوژنیک، مکانیستیک و تکاملی، هر یک مستقل از سایرین عمل میکند. از این میان مسیر های اتوترانسپورتری با توجه به سادگی آنها مورد توجه بیشتری قرار گرفته اند. به نمایش در آوردن(دیسپلی) پروتئین یا پپتیدی با عملکردی متفاوت از پروتئین های سطح سلولی سلول های زنده باکتریائی، چالشی است که دانشمندان و محققین علوم بیوشیمی و بیوتکنولوژی به صورت مداوم با آن روبرو هستند. سیستم اتودیسپلی براساس مکانیسم ترشحی خانواده اتو ترانسپورترها توسعه یافته است. مقالۀ حاضر به معرفی روش های جدید بیان پروتئین ها به روش اتودیسپلی و بر مبنای روش ترشح اتوترنسپورتر، در سطح سلول های باکتریائی پرداخته و قابلیت های بالقوۀ مختلف این روش و نیز کاربرد آن در تولید واکسن های نوترکیب جدید را شرح می دهد.

عنوان مقاله [English]

Applications of autodisplay system for recombinant protein expression on the bacterial cell surface

نویسنده [English]

  • R Pilechian Langroudi
Razi Serum and Vaccine Institute Karaj, Iran.
چکیده [English]

Protein secretory systems are found in all living organisms including gram negative bacteria, and eukaryotic cell’s organelles that are derived from these bacteria. Unlike all other living organisms, gram negative bacteria have different protein secretory systems. At least there are four different independent secretory systems, which are responsible to transport the secreted proteins to the milieu or insert them into the bacterial cell surface. Comprising these systems in bacteria suggests that, beside the biogenic, mechanistic and evolutionary similarities, each acting independently. To display a protein or peptide with a distinct function at the surface of a living bacterial cell, is a challenging exercise with constantly increasing impact in many areas of biochemistry and biotechnology. Autodisplay system is developed based on autotransporters. This article introduces autodisplay protein expression based on autotransporters, on the bacterial cell surface, and their different potential applications and also in vaccine production.

 

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