گزارش Anaplasma centrale سویه آموری در گاوهای ایران

نویسنده

بخش تحقیقات دامپزشکی مرکز تحقیقات کشاورزی و منابع طبیعی استان اصفهان، اصفهان، ایران

چکیده

150 نمونه خون از گاوها در  منطقه ای از اصفهان  تهیه شد.DNA استخراجی از نمونه های خونی توسط آغازگر اختصاصی A. centrale سویه آموری که بر اساس ژن 16S rRNA طراحی شده بود با روش Nested-PCR مورد بررسی قرار گرفت. گسترش های خونی از نظر A. centrale منفی بودند. نتایج نشان می دهند که از 150 نمونه مورد آزمایش با پرایمر اختصاصی A. centrale سویه آموری 2 نمونه (33/1درصد) مثبت بودند. این گزارش اولین تشخیص ملکولی  A. centrale سویه آموری در گاوهای ایران است.

عنوان مقاله [English]

Report of Anaplasma centrale (Amori strain) in cattle in Iran

نویسنده [English]

  • V Noaman
V. Department of Veterinary Research, Isfahan Research Center for Agriculture and Natural Resources, Isfahan, Iran
چکیده [English]

One hundred and fifty blood samples were prepared from cattle of a region in Isfahan. The extracted DNA from blood cells were analyzed by A. centrale (Amori strain) specific Nested-PCR using primers derived from 16S rRNA gene. All blood smears were negative for A. centrale like structures. The results showed that 2 of total 150 blood samples (1.33%) were A. centrale (Amori strain) positive by specific primers based on 16S rRNA gene. This report is the first molecular detection of A. centrale (Amori strain) from cattle in Iran.

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