جستجوی monocytogenes Listeria در موارد آندومتریت گاوهای شهرکرد به روش PCR

شناسنامه علمی شماره

نویسندگان

1 استادیار گروه علوم درمانگاهی دانشکده دامپزشکی و عضو پژوهشکده بیماری های مشترک انسان و دام دانشگاه شهرکرد

2 دانشجوی دانشکده دامپزشکی و عضو پژوهشکده بیماری های مشترک انسان و دام دانشگاه شهرکرد

3 دانشجوی دانشکده دامپزشکی دانشگاه شهرکرد

4 استادیار گروه مامایی و بیماری های تولیدمثل دام دانشکده دامپزشکی دانشگاه آزاد اسلامی واحد شهرکرد

چکیده

آندومتریت به عنوان یک ریسک فاکتور در ایجاد کیست تخمدانی، آنتروس و سایر مشکلات تناسلی مطرح می باشد و با اثر گذاری بر تولید شیر، به زیان های جدی اقتصادی منجر می شود.L. monocytogenes می‌تواند منجر به ایجاد آندومتریت گردد. با توجه به اهمیت آنچه ذکر گردید، مطالعة حاضر جهت ردیابی L. monocytogenes در موارد آندومتریت گاو در شهرکرد انجام شد. مجموعاً 180 نمونه از ترشحات رحمی به وسیلة سواب استریل از موارد آندومتریت گاو از شهرکرد جمع آوری شد. سواب ها مستقیماً درون  enrichment broth Listeria قرار داده شدند. سپس نمونه های غنی شده بر روی محیط Palcam agar به شکل خطی کشت داده شدند. کلنی های مشکوک به وسیله آزمون واکنش زنجیره ای پلی مراز (PCR) مورد ارزیابی قرار گرفتند. هیچ نمونة آلوده به L. monocytogenes یافت نشد. مطالعة حاضر نشان دهندة عدم حضور
L. monocytogenes در موارد آندومتریت گاو در این منطقه است. 
 

عنوان مقاله [English]

PCR detection of Listeria monocytogenes in bovine endometritis from Shahrekord, Iran

نویسندگان [English]

  • A Jafari Dehkordi 1
  • H Tahmasby 2
  • A Momeni 3
  • T Taktaz Hafshejani 4
1 Assistant Professor, Department of Clinical Science, Faculty of Veterinary Medicine and Member of Research Institute of Zoonotic Diseases, Shahrekord University, Shahrekord, Iran
2 Student, Faculty of Veterinary Medicine and Member of Research Institute of Zoonotic Diseases, University of Shahrekord, Shahrekord, Iran
3 Student, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran
4 Assistant Professor, Department of Veterinary Reproduction and Obstetrics, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
چکیده [English]

Endometritis as a risk factor for cystic ovarian disease, anoestrus and other reproductive disorder, is characterized by impact of dairy production performance and causes serious economic losses. Listeria monocytogenes can cause endometritis. Considering the importance of what mentioned present study was conducted to detection of L. monocytogenes in bovine endometritis from Shahrekord, Iran. Altogether 180 vaginal discharge samples of bovine endometritis were collected with sterile cotton swabs from Shahrekord, Iran. The swabs were placed directly into Listeria enrichment broth. Secondly enrichments were streaked on Palcam agar. Suspected colonies were evaluated for detecting L. monocytogenes by polymerase chain reaction (PCR) method. L. monocytogenes was not found in any samples. Present study suggests absence of L. monocytogenes in bovine endometritis in the region.

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