بررسی سرولوژیکی Neospora caninum درگاوهای شیری هلشتاین منطقه خرم آباد

نویسندگان

1 دانشجوی دکترای تخصصی،گروه انگل شناسی، دانشکده علوم پزشکی، دانشگاه تربیت مدرس

2 استاد، گروه انگل شناسی، دانشکده علوم پزشکی، دانشگاه تربیت مدرس

3 دانشیار، گروه انگل شناسی ، دانشکده علوم پزشکی، دانشگاه تربیت مدرس

4 دانشیار، گروه بیوتکنولوژی، دانشکده علوم پزشکی، دانشگاه تربیت مدرس

چکیده

 
N. caninum  انگل تک یاخته ای درون سلولی است که در سراسر جهان باعث سقط جنین در گاو می گردد.  این انگل حیواناتی مانند گاو، گوسفند و سگ را مبتلا می سازد. و یکی از مهمترین عوامل سقط جنین گاوی محسوب می شود. شیوع این انگل بر حسب گله، منطقه، کشور و نوع تست مورد استفاده متفاوت گزارش شده است. هدف از مطالعه حاضر بررسی میزان شیوع پادتن ضد N. caninum درسرم گاوهای شیری نژاد هلشتاین خرم آباد می باشد.  در این مطالعه از 181 راس گاو شیری خون گیری انجام و پس از جداسازی سرم، آلودگی به وسیله کیت تشخیصیELISA اختصاصی N. caninum مورد شناسایی قرار گرفت.طبق نتایج حاصله، تیترپادتن ضد N. caninum در 50 راس گاو (62/27 درصد) مثبت بوده است. با استفاده از آزمون مجذورکای هیچ گونه رابطه معنی داری بین میزان شیوع پادتن ضد N. caninum با سن گاوها مشاهده نشد. بعلاوه میزان آلودگی در گاوهای دارای سابقه سقط جنین حدود دو برابر گاوهای فاقد سابقه سقط جنین بوده است. با توجه به حضور سگ در همه گاوداری های مورد مطالعه، احتمال آلودگی گاوها به N. caninum از طریق سگ های نگهبان وجود دارد. 

عنوان مقاله [English]

Serological survey of Neospora caninum infection in Holstein dairy cattle from Khoramabad region

نویسندگان [English]

  • GH.A Sabevarinejad 1
  • A Dalimi 2
  • F Ghafarifar 3
  • M Forouzandeh-Moghadam 4
1 Department of Medical Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2 Department of Medical Parasitology, Faculty of Medical Sciences, Tarbiat Modares University.
3 Department of Medical Parasitology, Faculty of Medical Sciences, Tarbiat Modares University.
4 Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
چکیده [English]

Neospora caninum is an intracellular parasite which causes abortion in cattle worldwide. This organism infects a range of host species, including cattle, sheep and dogs. It is most important and a major cause of bovine abortion. Seroprevalence of infection in cattle appears to differ between herds, countries and regions, depending on the type of serologic test used. The aim of the present study was to determine the seroprevalence of N. caninum in cattle in Khoramabad region, west of Iran. Blood samples were collected from 181 cattle for determining the seroprevalence of N.caninum. A total of 181 serum samples were tested for anti-Neospora antibodies using a commercial N. caninum ELISA kit. Antibody against N. caninum was detected in 50 of 181 total cattle (27.62%). No significant difference was observed among N.caninum infection of different age groups of cattle. Furthermore, the infection rate in cows with a history of abortion was about twice the cows without history (P<0.01). Regarding the prevalence of infection and the presence of dogs in the whole dairy farms, the probability of transmission of Neospora infection from the guard dogs to cattle is estimated to be high.

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