اثر 4-n-نونیل فنل بر سنتز ویتلوژنین و غلظت هورمون های تیروئیدی در جنس نر نابالغ ماهی شانک زردباله (Acanthopagrus latus)

نویسندگان

1 گروه بیولوژی دریا، دانشکده علوم دریایی و اقیانوسی، دانشگاه علوم و فنون دریایی خرمشهر

2 گروه بیولوژی دریا، مرکز تحقیقات شیلات جنوب ایران، اهواز

3 گروه شیلات، دانشکده منابع طبیعی دریا، دانشگاه علوم و فنون دریایی خرمشهر

چکیده

نونیل فنل (NP) یک آلاینده استروژنیک بوده که با تقلید عملکرد هورمون طبیعی 17بتا-استرادیول (E2) موجب اختلال در سیستم اندوکرینی طیف وسیعی از موجودات می گردد. با این حال ایزومرهای مختلف این ماده توانایی های متفاوتی در بروز اثرات استروژنیک دارند. هدف از مطالعه حاضر بررسی اثر ایزومر NP با زنجیره جانبی مستقیم (n-NP- 4) برروی سنتز ویتلوژنین (VTG) و غلظت هورمون های تیروئیدی در ماهی شانک زردباله (Acanthopagrus latus) به عنوان پاسخ های استروژنیک و غیر استروژنیک سیستم اندوکرینی می باشد. بدین منظور تعداد 30 قطعه ماهی شانک زردباله نر نابالغ در خلال یک هفته دوزهای 10، 50 و 100 میکروگرم بر گرم از n-NP- 4 را از طریق تزریق درون صفاقی دریافت نمودند. القاء سنتز VTG در ماهیان تیمار شده با استفاده از سنجش فسفات باز ناپایدار و اندازه گیری سطوح کلسیم توتال پلاسما مورد بررسی قرار گرفت. غلظت پلاسمایی هومورن های تیروئیدی تیروکسین (T4) و تری یدوتیرونین(T3)  نیز به وسیله رادیوایمونواسی مورد سنجش قرار گرفت. نتایج نشان داد که n-NP- 4  موجب افزایش معنی دار سطح VTG در دوزهای 50 و 100 میکروگرم بر گرم می گردد ( 05/0>p). همچنین غلظت کلسیم توتال پلاسما در بالاترین دوز به کار رفته افزایش معنی داری را نسبت به گروه کنترل نشان داد (05/0>P). علاوه براین، غلظت هورمون T4 در دوزهای 50 و 100 میکرگرم بر گرم بطور معنی داری بالاتر از گروه کنترل بود (05/0>P). حال آنکه سطوح پلاسمایی T3 در ماهیان تیمار شده با بالاترین دوز  n-NP- 4 در مقایسه با گروه کنترل بطور معنی داری پایین تر بود (05/0>P). نتایج مطالعه حاضر نشان دهنده توانایی ترکیب  n-NP- 4 در ایجاد اثر استروژنیک و همچنین آشفتگی در تعادل هورمون های تیروئیدی ماهی شانک زردباله می باشد

عنوان مقاله [English]

The effect of 4-n-nonylphenol on vitellogenin synthesis and thyroid hormone concentrations in immature male yellowfin seabream (Acanthopagrus latus)

نویسندگان [English]

  • M Naderi 1
  • A.R Safahieh 2
  • S Dehghan Madiseh 2
  • E Rajabzade Ghatrami 3
  • H Zolgharnein 1
1 Department of Marine Biology, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran
2 Department of Marine Biology, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran
3 Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran
چکیده [English]

Nonylphenol (NP) is a well-known compound as having the potential to mimic the natural hormone 17β-estradiol (E2) and disrupt the endocrine system in a wide range of organisms. However, the ability of different isomers of this compound is different in exerting estrogenic effects. The objectives of this study were to estimate the effects of a straight side-chain NP isomer (4-n-NP) on the vitellogenin (VTG) synthesis and thyroid hormone concentrations as estrogenic and non-estrogenic responses of yellowfin seabream’s (Acanthopagrus latus) endocrine system. In this regard the total numbers of 30 immature male fish were intraperitonealy injectioned by 10, 50, 100 μg/g of 4-n- NP over a period of one week. The induction of VTG synthesis in treated fish was investigated using alkali labile phosphate assay and measurement of total plasma calcium. The concentrations of thyroid hormones, thyroxin (T4) and triiodothyronine (T3), in the plasma were also quantified by radioimmunoassay. The result showed that the VTG levels in the plasma were significantly increased by injection of 50 and and 100 μg/g of 4-n-NP (P<0.05). Moreover, the total plasma calcium concentrations in fish exposed to the highest dose used exhibited a significant increase compared to the control (P<0.05). In addition, T4 concentration in fish received 50 and 100 μg/g of 4-n-NP was significantly higher than the control group (P<0.05). While, the plasma levels of T3 in fish treated by 100 μg/g of 4-n-NP was significantly lower than controls (P<0.05). The results of present study indicate the potential of 4-n-NP to exert the estrogenic effect as well as disturbance in the balance of thyroid hormones in yellowfin seabream.

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