عنوان مقاله [English]
Detection of bluetongue virus in aborted lamb fetuses in Chaharmahal va Bakhtiari and Isfahan provinces, by RT-PCR method Background: Bluetongue or muzzle sore disease is an infectious disease which is mainly transmitted by insects. It can cause erosions in mouth, nasal mucosa and limbs and also abortion in ruminants. The aim of this study was genomic detection of bluetongue virus in sheep aborted fetuses as an aborting agent. Materials and methods: Samples were collected from blood, liver and spleen of 50 aborted lambs. EDTA was added to blood samples. RNA was extracted by using Rimazol. cDNA was synthesize was detected by RT-PCR. All products were separated by 1/5% agarose gel electrophoresis and stained in ethidium bromide. The results of all samples were negative, except one of them which was sent for sequencing and the result of sequencing didn't confirm presence of this virus. This study shows that Blue
tongue virus does not cause abortion in ewes in studied areas of Iran, but it is possible that by increasing the sample size and sampling during the year in these areas, the presence of virus or its genome can be detected too.
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