تولید آزمایشگاهی واکسن توکسوئید- باکترین پنج ظرفیتی کلستریدیائی و مقایسۀ آن با واکسن باکترین مرسوم

نوع مقاله: مقاله کامل

نویسندگان

1 موسسه تحقیقات واکسن و سرم سازی رازی،کرج، ایران

2 موسسه تحقیقات واکسن و سرم سازی رازی، کرج، ایران

چکیده

کلستریدیوم ها باکتری های بی هوازی گرم مثبت هاگ دار بوده و نقش مهمی در ایجاد بیماری های انسان و دام دارند. در این پژوهش سه گروه واکسن شامل، واکسن پنج ظرفیتی باکترین (کشت فرمله) تولید شده در شیشه های بیست لیتری و جذب شده روی یاور هیدروکسید آلمینیوم، واکسن پنج ظرفیتی باکترین تولید شده در فرمانتور تحقیقاتی و جذب شده روی هیدروکسید آلمینیوم و گروه سوم واکسن پنج ظرفیتی کشت توکسوئید-باکترین (واکسن اولترافیلتری) تولید شده در فرمانتور تحقیقاتی و جذب شده روی یاور هیدروکسید آلمینیوم در سطح آزمایشگاهی تولید و بررسی شد. کلیه آزمون های بیضرری، عدم وجود سمیت غیر عادی و توان ایمنی زائی، برای هر یک از سه نوع واکسن انجام شد. نتایج نشان داد که هر سه نوع واکسن کارآئی داشته و قابل استفاده اند ولی واکسن توکسوئید-باکترین تولید شده به وسیلۀ فرمانتور و اولترافیلتر، بهترین انتخاب برای تولید انبوه است.

کلیدواژه‌ها


عنوان مقاله [English]

A Production of pentavalent Clostridial toxoid vaccine and its comparison to conventional bacterin vaccine

نویسندگان [English]

  • R. Pilehchian Langroudi 1
  • A.R. Jabbari 2
  • M. Moosawi Shoshtary 1
  • A. Pardis 1
1 Razi Vaccine and Serum Research Institute, Karaj, Iran.
2 Razi Vaccine and Serum Research Institute, Karaj, Iran.
چکیده [English]

Genus Clostridium, gram positive spore forming anaerobes are very important bacteria for human and animals pathogenesis. Clostridial diseases frequently occurs in Iranian farms and different Clostridium species are responsible for high mortality in domestic animals like as sheep and goats. In the present study, three groups of experimental vaccines were produced and compared to each others. The 1st group as bacterin pentavalent vaccine was produced in 20 liters glass vessels and adsorbed on aluminium hydroxide adjuvant. The 2nd group as bacterin pentavalent vaccine has been produced within in 12 liters fermenter and adsorbed on aluminium hydroxide adjuvant. Finally, the 3rd group as bacterin-toxoid pentavalent vaccine was produced in 12 liters fermenter and adsorbed on aluminium hydroxide adjuvant. Analysis for freedom from abnormal toxicity, safety and potency tests were performed for all produced vaccine groups. The results showed that the 3rd group of produced vaccine by fermenter and purified by ultrafiltration system is the best choice for scaling up. 

کلیدواژه‌ها [English]

  • Pentavalent vaccine
  • Clostridium
  • Toxoid
  • Fermenter
  • Ultrafiltartion

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