عنوان مقاله [English]
Immunogen is a commercial prebiotic. It is a natural compound such as beta golcan and mannan oligosaccharide that use as a feed complement. Usage of prebiotic as indigestible nutrient in digestion system that potentially affect health condition of host is a new idea for improving production in aquaculture. The aim of this study is survey bacteria flora and body composition of Barbus grypus in 360 pcs fingerling with 35±2 gr weight in 4 treatment group randomly. (3 replicates) treatments were feed by Immunogen with zero, 0/5, 1 and 1/5% respectively in 90 days. Result shows that Immunogen has positive effect on bacterial flora in fingerling. Lactobacillus population in intestine of treatment groups in contrast with control group (p<0/05). Results showed that the amount of carcass protein in 1.5% treated Immunogen was significantly higher than control (p <0/05) where as lipid content, ash and moisture showed no significant differences between treatments (p> 0/05), although this factor was slightly higher in group fed with 1.5% Immunogen. Therefore it can be concluded that oral administration of prebiotic Immunogen can induces positive effects on microflore of gut and Lactobacillus population plus carcass protein level.
1- Association of Official Analytical Chemists (AOAC). (1990). Official methods of analysis of the association of official analytical chemists. 15th ed. Washington DC. USA.
2- Bailey, J., Blankenship, L. and Cox N. (1991). Effect of fructooligosaccharide on Salmonella colonization of the chicken intestine. Poult Sci. 70:2433-2438.
3- Burr, G., Hume, M., Neill, W. H. and Gatlin, D. M. (2008). Effects of prebiotics on nutrient digestibility of a soybean-meal-based diet by red drum Sciaenops ocellatus(Linnaeus). Aquaculture Research. 39:1680-1686.
4- Jenkins, D. J., Kendall, C. W. and Vuksan, V. (1999). Inuline, Oligofructose and Intestinal Function . J Nutr . 129:1431-1433.
5- Duncan, D. B. (1955). Multiple-range and multiple F tests. Biometrics 11, 1– 42.
6- Gatlin, D. M., Li, P., Wang, X., Burr, G. S., Castille, F. and Lawrence, A. L. (2006). Potential aplication of prebiotics in aquaculture, 8th International simposium on aquaculture nutrition, 371-376.
7- Heydari, M., Faridbakhsh, F., Mehrabi, Z. and Jafarpour, A. (2010). Effect of different levels sin biotic on performance and carcass characteristics of juveniles finger length of rainbow trout, 4th congress of Animal science, university of Tehran.
8- Hoseinifar S.H., Mirvaghefi A., Mojazi Amiri B., Khoshbavar-Rostami, H. and Merrifield D. 2010 . The effects of oligofructose on growth performance, survival, intestinal microbiota and liver histology of endangered great sturgeon (Huso huso) juvenile. Aquaculture Nutrition. 5:498-504.
9- Irianto, A. and B. Austin. (2002) . Probiotics in aquaculture. Journal of FishDiseases. 25:633-642.
10- Jafarzadeh, A., Soudagar, M., Aslanparviz, h. and Heydari, M. (2010). The Effect of the Commercial Prebiotic Immunogen_ on Growth Performance, Survival, Blood Indices and Intestinal Bacterial Flora of Persian Sturgeon Acipenser persicus. MSc thesis, Gorgan University, Gorgan, Iran (in Persian).
11- Kim, J. D. and Kaushik, S. J. (1992). Contribution of digestible energy from carbohydrate and estimation of protein / energy requirements for growth of rainbow trout. Aquaculture. 106:161-169.
12- Mahious, A. S. and Ollevier, F. (2005). Probiotic and Prebiotics in Aquaculture: Review. P 17-26.1.st Regional Workshop on Techniques for Enrichment of Live Food for Use in Larviculture (Uremia, Iran).
13- Mahious, A. S., Gatesoupe, F. J., Hervi, M., Metailler, R. and Ollevier, F. (2005). Effect of dietary inulin and oligosaccharides as prebiotics for weaning turbot, Psetta maxima. Aquaculture International. 14:219-229.
14- Ojifar, A., Abedin-Kenari, A. M., Nafisi-Bahabadi, M. and Abbaszadeh, A. (2008). The effect of dietary inulin on body fatty acid composition of pacific white shrimp (Litopenaeus vannamei). The First National Conference on Fisheries Sciences & Aquatic Organisms, Lahijan, Iran. (InPersian). 13-15.
15- Panigrahi, A., Kiron, V., Satoh, S., Hirono, I., Kobayashi, T., Sugita, H., Puangkaew J. And Aoki, T. (2007). Immune modulation and expression of cytokine genes in rainbow trout Oncorhynchus mykiss upon probiotic feeding. Fish and Shellfish Immunology. 31:372-382.
16- Ringo, E. and Gatesoupe, F. J. (1998). Lactic acid bacteria in fish: a review. Aquaculture. 160:177-203.
17- Ringo, E. and Birkbeck,T. H. (1999). Intestinal micro flora of fish larvae and fry. Aquaculture. 30: 73-93.
18- Ringo, E., Sperstad, S., Myklebust, R., Mayhew, T. M. and Olsen, R. E. (2006). The effect of dietary inulin on aerobic bacteria associated with hindgut of Arctic charr (Salvelinus alpinus). Aquaculture Research. 37:891- 897.
19- Salze, G., McLean, E., Schwarz, M.H. and Craig, S.R. (2008). Dietary mannan oligosaccharide enhances salinity tolerance and gut development of larval cobia. Aquaculture. 274:148–152.
20- Schley, P. D. and Field, C. J. (2002). The immune-enhancing effects of dietary fibers and prebiotics. British Journal Nutrition. 87:221-230.
21- Sheikholeslami, M., Yusefian, M., Yavari, V., Mohamadian, T., Abhari, H. and Goran, H. (2007). Modulation of rainbow trout immune system and enhance resistance against streptococosis using dietary Inulin, The first national conference on Caspian Sea fisheries resources. Gorgan, Iran: Gorgan University.
22- Staykov, Y., Spring, P., Denev, S. and Sweetman, J. (2007). Effect of a mannan oligosaccharide on the growth performance and immune status of rainbow trout (Oncorhynchus mykiss). Aquaculture Int. 15:153-161.
23- Sudagar, M., Imanpoor, M., and Hoseinifar, S. H. (2004). Effect of optimum (Ascogen or Vannagen) growth stimulant supplementation on the growth and survival rate of grand beluga juvenile (Huso huso). Iranian Journal of Marine Science. 3:33-38.
24- Torrecillas, S., Makol, A., Caballero, M. J., Montero, D., Robaina, L., Real, F., Sweetman, J., Tort, L. and Izquierdo, M.S., 2007. Immune stimulation and improved infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides. Journal of Fish & Shellfish Immunology. 23:981-969.
25- Zaccorate, I. Gasco, L. Sicuro, B. Palmegiano, G. B. and Luzzana, U. (1996). Use of by-product frpm poultry slaughtering in rainbow trout (Oncorhynchus mykiss). Rivista Italiana diaquacoltura. 31:145-156.
26- Zhou, Z., Shi, P., Yao, B., He, S. and Su, Y. (2007). Comparison of the predominant bacterial community structure in the gastrointestinal wall between Lutjanus sebae and Ephippus orbis based on 16S rDNA PCR-DGGE fingerprint. Acta Hydrobiol. Sin. 31: 78-84.